Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock☆

Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection

“…During screening of a subtracted cDNA library that was constructed with mRNA made from UV-inactivated SMRV-infected and mock-infected FEC cells [35], one EST homologous to mammalian PRMT1 gene was retrieved. A pair of primers were designed (Table 1) and used to amplify both 5 0 and 3 0 ends of the gene corresponding to the EST from a SMART cDNA library [36].…”

“…Nucleic acid concentrations were measured at 260 nm. First-strand cDNA was synthesised according to the RevertAid H Minus First Strand cDNA Synthesis Kit (Fermentas) [35]. Real-time PCR was performed by DNA Engine Chromo 4 Real-time System (MJ Research) with SYBR Green I Dye according to a previous report [35].…”

“…First-strand cDNA was synthesised according to the RevertAid H Minus First Strand cDNA Synthesis Kit (Fermentas) [35]. Real-time PCR was performed by DNA Engine Chromo 4 Real-time System (MJ Research) with SYBR Green I Dye according to a previous report [35]. PCR conditions were as follows: 94 C for 3 min, 94 C for 20 s and 56 C for 20 s, and 72 C for 20 s for 40 cycles, followed by 72 C for 10 min.…”

Genome-wide identification of Hsp70 genes in channel catfish and their regulated expression after bacterial infection

“…In teleost, hsp70 genes have been found to be involved in bacterial kidney disease in coho salmon [28] and vibriosis in rainbow trout [29]. In olive flounder, Hsp40 proteins were found to be upregulated in flounder embryonic cells (FEC) after viral infection and a flounder hsp70 gene was also expressed in heat-shocked and virus treated FEC cells [30], indicating hsp40 and hsp70 functioned as co-chaperone in antiviral immune responses. In the kidney of olive flounder, dnaja4, dnajb6 and dnajb11 were found to be expressed after being infected by Streptococcus parauberis [31].…”

Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection

“…Using flounder as a experimental model to study stress-induced immune response, we have cloned several anti-viral and immune related genes such as Mx, Hsp40 and PEDF [22,23]. In this study, we characterized a fish VDAC homolog, Paralichthys olivaceus PoVDAC, analyzed its differential expression in healthy flounder tissues and virusinfected FEC cells, the subcellular distribution was displayed by immunofluorescence staining using fish antiserum.…”