Differential Expression of TXNIP Isoforms in the Peripheral Leukocytes of Patients with Acute Myocardial Infarction

Abstract

Background Acute myocardial infarction (AMI) is the most serious type of coronary atherosclerotic heart disease (CAD). The pathological changes are characterized by atherosclerosis. Oxidative stress plays an important role in atherosclerosis. Thioredoxin-interacting protein (TXNIP), an endogenous inhibitor and regulator of thioredoxin, could bind thioredoxin to regulate its expression and antioxidant activity negatively. The NCBI data show that there are two isoforms in TXNIP gene, namely, TXNIP1 and TXNIP2. Our previous studies have shown that TXNIP expression levels in patients with unstable angina pectoris (UAP) were increased compared with controls (CTR). However, no upregulation of TXNIP was detected in AMI patients. Methods The leucocytes were isolated from peripheral venous blood, and total RNA of the leucocytes was extracted. Then, real-time quantitative PCR was performed. Results mRNA levels of TXNIP2 in AMI were significantly increased compared with CTR (P < 0.05). However, the expression of TXNIP1 was downregulated in AMI, but the difference was not statistically significant (P > 0.05). Logistic regression analysis showed that TXNIP2 mRNA levels were significantly associated with AMI (OR = 2.207, P < 0.05). Conclusions The expression of TXNIP2, not TXNIP1, is upregulated in leukocytes of AMI patients, indicating that only TXNIP2 in circulating leucocytes may be involved in the pathogenesis of AMI.

Extracellular vesicles derived from microRNA-150-5p-overexpressing mesenchymal stem cells protect rat hearts against ischemia/reperfusion

“…Overexpression of TXNIP increases the sensitivity of cardiomyocytes to oxidative stresses [ 19 ]. TXNIP was also upregulated in the peripheral leukocytes of patients with myocardial infarction or patients with coronary atherosclerotic heart disease, which suggests that circulating leucocytes also responded to the oxidative stress in the blood and participated in the pathogenesis of heart disease [ 20 , 21 ]. Therefore, TXNIP might be considered as a biomarker in myocardial I/R injury considering its role on oxidative stresses.…”

The Emerging Role of TXNIP in Ischemic and Cardiovascular Diseases; A Novel Marker and Therapeutic Target

“…At-risk Takayasu arteritis, atherosclerosis, coronary artery disease, leukostasis [80,94,110,[200][201][202][203] Unstable angina pectoris, acute myocardial infarction [29,204] Diabetes associated macrovascular endothelial dysfunction [17] Diabetes associated vascular complications [19,205] TXNIP in cardiac tissue Heart attack [55] Even though a recent genetic study in a family with homozygous nonsense mutations shows that the suppression of TXNIP expression is non-lethal in humans, functional variants are reportedly associated with disease in the literature. Two different genetic variants of TXNIP can be described as genetic markers for cardiovascular risk.…”

“…Interestingly, this TXNIP expression in peripheral blood mononuclear cells is associated with higher TXNIP expression in the aortic walls of these patients [ 200 ]. More specifically, TXNIP mRNA levels in leucocytes have also been investigated and are increased in patients with unstable angina pectoris [ 29 ] or with acute myocardial infarction [ 204 ], suggesting the role of TXNIP in atherosclerosis and the pathogenesis of cardiovascular diseases. Even if the endothelial levels of TXNIP are correlated with the adhesion of leucocytes, TXNIP levels in leucocytes also have an impact on the physiopathology of atherosclerosis [ 94 , 201 ].…”

Thioredoxin‐interacting protein promotes activation and inflammation of monocytes with DNA demethylation in coronary artery disease

“…Various studies showed that TXNIP was overexpressed in peripheral blood leucocytes (PBLs) of CAD patients . However, the specific mechanism of it remained unclear.…”

“…20 Various studies showed that TXNIP was overexpressed in peripheral blood leucocytes (PBLs) of CAD patients. [21][22][23] However, the specific mechanism of it remained unclear. Recent researches revealed that cg19693031, a methylated position at the 3′ UTR of TXNIP, was associated with type 2 diabetes (T2DM).…”