Differential expression of vasoactive intestinal peptide receptors 1 and 2 (VIP-R1 and VIP-R2) mRNA in murine lymphocytes

Delgado, Mario; Martı́nez, Carmen; Johnson, Mary C.; Gomariz, Rosa P.; Ganea, Doina

doi:10.1016/0165-5728(96)00063-x

Cited by 106 publications

(84 citation statements)

References 57 publications

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“…Vpr2 levels were higher than Vpr1 in CD4 ϩ CD8 ϩ thymocytes, the largest population in the thymus. In agreement with previous reports regarding Vpr1 expression in rat (Gomariz et al, 1994a) and mouse (Delgado et al, 1996b), splenic CD4 ϩ and CD8 ϩ T cells express Vpr1 (Pankhaniya et al, 1998). The dis-VIP IN IMMUNOMODULATION crepancies observed in Vpr1 and Vpr2 expression patterns could be attributed to different primers, especially when dealing with negative results.…”

Section: B Biochemical Pharmacological and Signaling Key Features supporting

confidence: 79%

“…ϩ T and CD8 ϩ T cells, Vpr2 mRNA is induced only after stimulation with anti-CD3 and PMA (Delgado et al, 1996b). B cells isolated from murine splenocytes did not express either Vpr1 or Vpr2 in resting or after LPS challenge, suggesting species differences as Vpr1 mRNA were detected in rat B cells from spleen, thymus, and lymph nodes (Gomariz et al, 1994a).…”

Section: B Biochemical Pharmacological and Signaling Key Features mentioning

confidence: 99%

“…Vpr2 transcripts are detected in bulk murine thymocytes and murine splenic CD4 ϩ and CD8 ϩ T cells only after TCR engagement and PMA treatment, whereas Vpr1 mRNA is constitutively expressed (Delgado et al, 1996b). VIP has an antiproliferative effect on T cells through cAMP, and VPAC 1 induces higher cAMP levels than VPAC 2 .…”

Section: B Biochemical Pharmacological and Signaling Key Features mentioning

confidence: 99%

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The Significance of Vasoactive Intestinal Peptide in Immunomodulation

Delgado

Pozo²,

Ganea³

2004

Pharmacol Rev

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372

319

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Section: B Biochemical Pharmacological and Signaling Key Features supporting

confidence: 79%

Section: B Biochemical Pharmacological and Signaling Key Features mentioning

confidence: 99%

Section: B Biochemical Pharmacological and Signaling Key Features mentioning

confidence: 99%

See 1 more Smart Citation

The Significance of Vasoactive Intestinal Peptide in Immunomodulation

Delgado

Pozo²,

Ganea³

2004

Pharmacol Rev

Self Cite

372

319

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“…Previous data in rodent and human myeloid and lymphoid cells have also shown a different distribution and regulation of VPAC 1 and VPAC 2 (33,34). Monocytes and T cells also constitutively express VPAC 1 , whereas VPAC 2 is inducible by specific cell activation (35)(36)(37). VPAC 1 receptor has also been preferentially detected in CD34ϩ,CD38Ϫ primitive hematopoietic stem cells (38).…”

Section: Discussionmentioning

confidence: 92%

Differential expression of vasoactive intestinal peptide and its functional receptors in human osteoarthritic and rheumatoid synovial fibroblasts

Juarranz

Gutiérrez‐Cañas

Santiago

et al. 2008

Arthritis & Rheumatism

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Objective. Vasoactive intestinal peptide (VIP) hasshown potent antiinflammatory effects in murine arthritis and ex vivo in human rheumatoid arthritis (RA) synovial cells. To investigate the potential endogenous participation of this system in the pathogenesis of RA, we analyzed the expression and regulation of VIP and its functional receptors in human fibroblast-like synoviocytes (FLS) from patients with osteoarthritis (OA) and patients with RA.Methods. The expression of VIP was studied by reverse transcription-polymerase chain reaction (RT-PCR), enzyme immunoassay, and immunofluorescence in cultured FLS, and by immunohistochemical analysis in synovial tissue. The expression and function of the potential VIP receptors in FLS were studied by RT-PCR, determination of intracellular cAMP production, cell membrane adenylate cyclase (AC) activity, and interleukin-6, CCL2, and CXCL8 production in response to VIP or specific agonists and antagonists.Results. VIP expression was detected in human FLS at the messenger RNA and protein levels, and it was significantly decreased in RA FLS compared with OA FLS. VIP receptor type 1 (VPAC 1 ) was the dominant AC-coupled receptor in OA FLS, in contrast with RA FLS, in which VPAC 2 was dominant. Tumor necrosis factor ␣-treated OA FLS reproduced the VIP and VPAC receptor expression pattern of RA FLS. The antagonistic effects of VIP on FLS proinflammatory factor production were reproduced by VPAC 1 -and VPAC 2 -specific agonists in OA FLS and RA FLS, respectively.Conclusion. VIP expression is down-regulated in RA and in tumor necrosis factor ␣-treated FLS, suggesting that down-regulation of this endogenous antiinflammatory factor may contribute to the pathogenesis of RA. In RA FLS, VPAC 2 mediates the antiinflammatory effects of VIP, suggesting that VPAC 2 agonists may be an alternative to VIP as antiinflammatory agents.The vasoactive intestinal peptide (VIP)/pituitary adenylate cyclase-activating peptide (PACAP) system consists of 2 peptides and 3 receptors: VIP receptor type 1 (VPAC 1 ), VPAC 2 , and PACAP type 1 (PAC 1 ) receptor. These receptors belong to family 2 of the G proteincoupled receptors, which in recent years have shown remarkable antiinflammatory and immunomodulatory properties (1-3). VPAC 1 is constitutively expressed in macrophages and lymphocytes and binds VIP and PACAP with equal affinity (1,3). VPAC 2 has also been described in lymphocytes and macrophages as an inducible receptor after T cell receptor triggering or lipopolysaccharide (LPS) stimulation (1,3). The bestcharacterized effects of VIP/PACAP on immune cells are mediated by the adenylate cyclase (AC) pathway coupled to these receptors. Finally, the PAC 1 receptor is the PACAP-specific receptor, although at high concentrations VIP is a heterologous ligand (4). Endogenous VIP and PACAP can be produced by both neural and immune cells, but their regulation and participation in the pathogenesis of human inflammation are not known.

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“…neurotransmitters as well as neuropeptides/neuropeptide receptors have been applied in immunohistochemical studies in rats (Gomariz et al 1990;Kendall and al-Shawaf 1991;Bellinger et al 1997), mice (Delgado et al 1996;Mitchell et al 1997), and humans (Lara-Marquez et al 2000;Mignini et al 2011). Experimental procedures like chemical sympathectomy with 6-hydroxydopamine (6-OHDA) or guanethidine , surgical denervation of the sympathetic chain, ablation of the vagus or recurrent laryngeal nerve (Mignini et al 2010) allowed to determine preliminary sources of sympathetic and parasympathetic innervation to the rat thymus.…”

mentioning

confidence: 99%

The localization of primary efferent sympathetic neurons innervating the porcine thymus – a retrograde tracing study

2017

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The autonomic nervous system is a sophisticated and independent structure composed of two antagonistic (opposing) divisions (sympathetic and parasympathetic) that control many vital functions including: homeostasis maintenance, heart rate, blood circulation, secretion, etc. Thymus is one of the most important primary lymphoid organs playing a role in the developing of a juvenile's immune system mainly by maturation, development, and migration of T-cells (T lymphocytes). In the last decades, several studies identifying sources of the thymic autonomic supply have been undertaken in humans and several laboratory rodents but not in higher mammals such as the pig. Therefore, in the present work, retrograde tracing technique of Fast Blue and DiI was used to investigate the sources of sympathetic efferent supply to the porcine thymus. After Fast Blue injection into the right lobe of the thymus, the presence of Fast Bluepositive neurons was found in the unilateral cranial cervical ganglion (82.8 ± 3.0% of total Fast Blue-positive neurons) as well as in the middle cervical ganglion (17.2 ± 3.0%). Injection of DiI resulted in the presence of retrograde tracer in neurons of the cranial cervical ganglion (80.4 ± 2.3% of total amount of DiI-labelled neurons), the middle cervical ganglion (18.4 ± 1.9%), and the cervicothoracic ganglion (1.2 ± 0.8%). The present report provides the first data describing in details the localization of primary efferent sympathetic neurons innervating the porcine thymus.

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Differential expression of vasoactive intestinal peptide receptors 1 and 2 (VIP-R1 and VIP-R2) mRNA in murine lymphocytes

Cited by 106 publications

References 57 publications

The Significance of Vasoactive Intestinal Peptide in Immunomodulation

The Significance of Vasoactive Intestinal Peptide in Immunomodulation

Differential expression of vasoactive intestinal peptide and its functional receptors in human osteoarthritic and rheumatoid synovial fibroblasts

The localization of primary efferent sympathetic neurons innervating the porcine thymus – a retrograde tracing study

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