Differential expression of VEGF isoforms in mouse during development and in the adult

Ng, Y. Jack; Rohan, Richard M.; Sunday, Mary E.; deMello, Daphne E.; D’Amore, Patricia A.

doi:10.1002/1097-0177(2000)9999:9999<::aid-dvdy1093>3.0.co;2-d

Cited by 344 publications

(291 citation statements)

References 23 publications

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“…It is during this stage of development that alveolar-capillary contacts are established. Our data along with other reports (Healy et al, 2000, Bhatt et al, 2000Ng et al, 2001), suggest that focal expression of VEGF-A is critical to this process.…”

Section: Discussionsupporting

confidence: 88%

“…Figure 3 shows low but constant VEGF-A mRNA expression in the mesenchyme throughout lung development. As reported by Gebb and Shannon (2000) and Ng et al (2001), epithelial expression of VEGF-A was detected during the late pseudoglandular stage of lung development, E14.5 to E16.5. At E14.5, there were a few focal areas of distal epithelium with high expres- Fig.…”

Section: Vegf-d and Vegf-a Localize To Different Compartments During supporting

confidence: 76%

“…The three predominant isoforms in mouse are VEGF-A120, VEGF-A164, and VEGF-A188, with increasing affinity for heparan sulfates, respectively. The relative abundance of VEGF splice variants changes during lung development (Watkins et al, 1999;Ng et al, 2001). The differences in mRNA size among the splice variants are too small to be detected by Northern analysis; therefore, to refine the pattern of each VEGF-A splice variant in developing mouse lung, we used reverse transcriptase-polymerase chain reaction (RT-PCR) analysis.…”

Section: Heparin Bound Vegf-a Variant Is First Expressed In Developinmentioning

confidence: 99%

“…4). Ng et al (2001) used an isoform-specific probe to demonstrate that pulmonary epithelial cells express V188. The induction of the V188 splice variant that we observed shortly after E16.5 ( Fig.…”

Section: Vegf-d and Vegf-a Localize To Different Compartments During mentioning

confidence: 99%

“…These properties suggest each isoform may have a unique role in vascular development. Ratios of mRNA for the three isoforms change during lung development, with expression of the diffusable isoforms V120 and V164 high early and the expression of heparan sulfate binding isoform V188 high just before birth (Ng et al, 2001). All three isoforms are found in the adult lung (Miquerol et al, 1999;Healy et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Mesenchymal expression of vascular endothelial growth factors D and A defines vascular patterning in developing lung

Greenberg¹,

Thompson²,

Brooks³

et al. 2002

Developmental Dynamics

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The lung has specific vascular patterning requirements for effective gas exchange at birth, including alignment of airways and blood vessels and lymphatic vessels. Vascular endothelial growth factors (VEGF) are potent effectors of vascular development. We examined the temporal and spatial expression of VEGF-D and specific VEGF-A isoforms at each stage of lung development. VEGF-D, expressed only by cadherin-11-positive cells of the mesenchyme, is first detected at embryonic day (E) 13.5, a period of active vasculogenesis. VEGFR-3, its cognate receptor, is detected earlier on days E11.5 to E14.5, in both blood vessels and lymphatic vessels and later, on day E17.5, in only lymphatic vessels. VEGF-A is expressed in the mesenchyme throughout lung development and also by the epithelium midway through organogenesis. Before E14, the predominant forms of VEGF-A are the soluble isoforms, VEGF-A120 and 164. Not until E14.5 do epithelial cells at the tips of expanding airways express VEGF-A, including VEGF-A188, an isoform with high affinity for extracellular matrix. Our results demonstrate unique temporal and spatial expression of VEGF-D and specific VEGF-A isoforms during lung development and suggest these related factors have distinct functions in vascular and lymphatic patterning of the lung.

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Section: Discussionsupporting

confidence: 88%

Section: Vegf-d and Vegf-a Localize To Different Compartments During supporting

confidence: 76%

Section: Heparin Bound Vegf-a Variant Is First Expressed In Developinmentioning

confidence: 99%

Section: Vegf-d and Vegf-a Localize To Different Compartments During mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mesenchymal expression of vascular endothelial growth factors D and A defines vascular patterning in developing lung

Greenberg¹,

Thompson²,

Brooks³

et al. 2002

Developmental Dynamics

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Lung Development

Havrilak

Shannon

2015

Encyclopedia of Life Sciences

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Early respiratory development begins with the specification of the lung cell fate, followed by the emergence of lung buds from the ventral foregut endoderm. The respiratory tree is then elaborated through branching morphogenesis, and proliferation and differentiation of specialised cell types along the proximal–distal axis produces airways and alveoli, respectively. Lung development is orchestrated through crosstalk between the epithelium and the mesenchyme; morphogenesis and differentiation of the lung relies on diffusible signalling molecules that cross tissue layers to activate a complex network of transcription factors to drive development. Significant progress has been made in understanding the molecular regulation of lung morphogenesis through paracrine signalling pathways and transcription factors essential for lung development. Key Concepts Following specification of respiratory progenitor cells that are committed to the respiratory lineage, the lung buds emerge from the ventral foregut endoderm and subsequently elaborate the pulmonary tree through branching morphogenesis. Reciprocal interactions between the epithelium and the mesenchyme govern lung organogenesis and are mediated by diffusible factors. Critical signalling pathways known to be involved in lung development include Fgfs, Shh, Wnts, RA and TGF‐β superfamily. These factors are diffusible and act as means of communication and instruction between the tissue layers to drive development. The lung comprises specialised cell types that function to protect the lung, secrete surfactant or allow for gas exchange.

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Development of the Pulmonary Endothelium in Development of the Pulmonary Circulation: Vasculogenesis and Angiogenesis

Schwarz

Cleaver²

2009

The Pulmonary Endothelium

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Differential expression of VEGF isoforms in mouse during development and in the adult

Cited by 344 publications

References 23 publications

Mesenchymal expression of vascular endothelial growth factors D and A defines vascular patterning in developing lung

Mesenchymal expression of vascular endothelial growth factors D and A defines vascular patterning in developing lung

Lung Development

Development of the Pulmonary Endothelium in Development of the Pulmonary Circulation: Vasculogenesis and Angiogenesis

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