Differential expression profile of MAGE family in non-small-cell lung cancer

Abstract. The aim of this study was to detect differences in the expression levels of melanoma-associated antigen D4 (MAGED4) mRNA between non-small cell lung cancer (NSCLC) tissues and normal tissues, and to compare differences in the expression levels of MAGED4 in tumor patients. Patients were grouped according to age, gender, smoking history, tumor size, pathological classification, degree of lung cancer cell differentiation and presence of lymph node metastasis. The expression levels of MAGED4 were detected using real-time fluorescence quantitative PCR. MAGED4 expression was higher in squamous cell carcinomas compared to adenocarcinomas (P<0.05), in poorly differentiated tissues compared to well-differentiated tissues (P<0.05), and in patients with lymph node metastasis compared to patients without lymph node metastasis (P<0.05). MAGED4 may be used as a specific antigen for NSCLC to influence the improvement of diagnosis, prognosis and immunological therapy outcomes in lung cancer patients.

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“…These results were in accordance with the properties of other members of the MAGE gene family discovered in previous studies (15,17,18).…”

Section: Discussionsupporting

confidence: 93%

“…However, findings obtained by other studies vary widely. One study revealed that MAGE-A3 expression levels in lung cancer were only 13% (18). Identification of a consistent lung cancer-specific antigen remains an aim for researchers in the field.…”

Section: Discussionmentioning

confidence: 99%

The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCR

Pang

Chen

et al. 2012

Oncology Letters

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“…13,14 Clinical Study Co-expression patterns of tumor-associated antigen genes by non-small cell lung carcinomas: Implications for immunotherapy Published expression patterns, on the other hand, refer only to TAAs of particular families. 15,16 To this end, we draw a caveat in TAA expression frequencies in cancer, including NSCLC, since for their majority a wide variability has been reported, mainly due to either the different techniques utilized for their assessment or racial differences that could exist. This study was performed in an attempt to investigate whether such a TAA co-expression pattern exists in NSCLC.…”

Section: Introductionmentioning

confidence: 99%

Co-expression patterns of tumor-associated antigen genes by non-small cell lung carcinomas: Implications for immunotherapy

Karanikas

Tsochas

Boukas

et al. 2008

Cancer Biology & Therapy

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“…Over 32 MAGE proteins have been identified in humans, but the normal physiological function of this family remains unclear (7). Members of the hMAGE-A, hMAGE-B and hMAGE-C subfamilies are only expressed in male germ cells or placenta, but completely silent in most normal tissues (5,8).…”

Section: Introductionmentioning

confidence: 99%

“…Members of the hMAGE-A, hMAGE-B and hMAGE-C subfamilies are only expressed in male germ cells or placenta, but completely silent in most normal tissues (5,8). However, many members of the MAGE family are expressed in various tumor cells and thus are of particular interest for antitumor immunotherapy (7). Members of the hMAGE-D, hMAGE-E, hMAGE-F, hMAGE-G and hMAGE-H subfamilies are expressed in many normal tissues at various levels (5,9,10).…”

Section: Introductionmentioning

confidence: 99%

MAGE-D1 inhibits proliferation, migration and invasion of human breast cancer cells

Tian¹

2009

Oncol Rep

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Abstract. MAGE-D1, also known as NRAGE or Dlxin-1, is a member of the MAGE family of proteins. It interacts with multiple adaptors and mediates various cellular functions such as regulation of apoptosis, transcription, cell cycle, cell adhesion and angiogenesis. In this study, we evaluated the effect of MAGE-D1 plasmid transfection on the growth, migration and invasion of breast cancer cells. MTT assay and cell counting consistently showed that MAGE-D1 transfection could effectively inhibit the proliferation of breast cancer cells. However, further FACS analyses failed to demonstrate any alterations in cell cycle distribution and apoptosis after MAGE-D1 transfection. In vitro scratch wound healing assay exhibited that MAGE-D1 suppressed cell migration. In addition, Boyden chamber invasion assay showed that MAGE-D1 significantly inhibited cell invasion. Furthermore, in an attempt to elucidate the mechanism of MAGE-D1 in suppressing cellular growth and invasion, the protein expressions of p53, p21, E-cadherin, ß-catenin, MMP-2 and MMP-9 were assessed. Western blotting showed that MAGE-D1 upregulated the expression of p53, p21 and E-cadherin, whereas down-regulated ß-catenin expression. Taken together, this study suggests that MAGE-D1 play important roles in the regulation of cell proliferation, migration and invasion of breast cancer cells. Enhanced expression of MAGE-D1 by gene transfer could reverse the malignant phenotypes of breast cancer cells. MAGE-D1 may be a potential therapeutic target for breast cancer.

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Differential expression profile of MAGE family in non-small-cell lung cancer

Cited by 54 publications

References 35 publications

The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCR

The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCR

Co-expression patterns of tumor-associated antigen genes by non-small cell lung carcinomas: Implications for immunotherapy

MAGE-D1 inhibits proliferation, migration and invasion of human breast cancer cells

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