Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Fanalli, Simara Larissa; Silva, Bruna Pereira Martins da; Gomes, Julia Dezen; Almeida, Vivian Vezzoni de; Freitas, Felipe André Oliveira; Moreira, Gabriel Costa Monteiro; Silva-Vignato, Bárbara; Afonso, Juliana; Reecy, James M.; Koltes, James E.; Koltes, Dawn; Regitano, L. C. de A.; Garrick, Dorian J.; Balieiro, Júlio César de Carvalho; Meira, Ariana Nascimento; Freitas, Luciana; Coutinho, Luiz Lehmann; Lesbon, Jéssika Cristina Chagas; Mourão, Gerson Barreto; Alencar, Severino Matias de; Filho, Albino Luchiari; Cesar, Aline Silva Mello

doi:10.3390/ani12131632

Cited by 7 publications

(12 citation statements)

References 94 publications

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“…Most serum parameters showed no difference with the addition of soybean oil to the diet as shown in our previous study [32]. With the increase in oil, we observed lower values of albumin, triglycerides and VLDL.…”

Section: Different Levels Of Dietary Soybean Oil Modulates Fat Deposi...supporting

confidence: 76%

“…Each pen was equipped with a three-hole dry self-feeder and a nipple drinker, allowing pigs ad libitum access to feed and water throughout the experimental period. Immunocastration of the intact males was performed by the administration of two 2-mL dose of Vivax® (Pfizer Animal Health, Parkville, Australia) on day 56 (127 days of age) and day 70 (141 days of age) [31,32], in accordance with the manufacturer's recommendations.…”

Section: Animalsmentioning

confidence: 99%

“…Sample collection fatty acid profile was previously described in Almeida et al, [31], and Fanalli et al, [32]. In summary, liver and skeletal muscle (Longissimus lumborum) samples were collected, and then stored at -80 °C until fatty acid profile and RNA sequencing analyses.…”

Section: Fatty Acid Profilementioning

confidence: 99%

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Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Fanalli¹,

Silva²,

Gomes³

et al. 2022

Preprint

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The aim of this study was to identify the differentially expressed genes (DEG) from the skeletal muscle and liver samples of animal model for metabolic diseases in human. To perform the study, the fatty acid (FA) profile and RNA sequencing (RNA-Seq) data of 35 samples of liver tissue (SOY1.5, n=17 and SOY3.0, n=18) and 36 samples of skeletal muscle (SOY1.5, n=18 and SOY3.0, n=18) of Large White pigs were analyzed. The FA profile of the tissues was modified by the diet, mainly those related to monounsaturated (MUFA) and polyunsaturated (PUFA) FA. The skeletal muscle transcriptome analysis revealed 45 DEG (FDR 10%), and the functional enrichment analysis identified network maps related to inflammation, immune process, and pathways associated with the oxidative stress, type 2 diabetes and metabolic dysfunction. For the liver tissue, the transcriptome profile analysis revealed 281 DEG, which participate in network maps related to neurodegenerative diseases. With this nutrigenomics study, we verified that different levels of soybean oil in the pig diet, an animal model for metabolic diseases in humans, affected the transcriptome profile of skeletal muscle and liver tissue. These findings may help to better understand the biological mechanisms that can be modulated by the diet.

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Section: Different Levels Of Dietary Soybean Oil Modulates Fat Deposi...supporting

confidence: 76%

Section: Animalsmentioning

confidence: 99%

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Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Fanalli¹,

Silva²,

Gomes³

et al. 2022

Preprint

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show abstract

“…According to Stelzer et al [ 18 ], among the pathways associated with this gene there were “eicosanoid synthesis” and “arachidonic acid metabolism” and the related Gene Ontology (GO) annotations include “calcium ion binding” and “lipid binding”. Fanalli et al [ 19 ] demonstrated that the addition of soybean oil in the diet of pigs in different proportions acted on the modulation of genes, pathway maps and networks associated with inflammation, immune response, oxidative stress, and neurodegenerative diseases, in muscle and liver.…”

Section: Discussionmentioning

confidence: 99%

Brain fatty acid and transcriptome profiles of pig fed diets with different levels of soybean oil

et al. 2023

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Background The high similarity in anatomical and neurophysiological processes between pigs and humans make pigs an excellent model for metabolic diseases and neurological disorders. Lipids are essential for brain structure and function, and the polyunsaturated fatty acids (PUFA) have anti-inflammatory and positive effects against cognitive dysfunction in neurodegenerative diseases. Nutrigenomics studies involving pigs and fatty acids (FA) may help us in better understanding important biological processes. In this study, the main goal was to evaluate the effect of different levels of dietary soybean oil on the lipid profile and transcriptome in pigs’ brain tissue. Results Thirty-six male Large White pigs were used in a 98-day study using two experimental diets corn-soybean meal diet containing 1.5% soybean oil (SOY1.5) and corn-soybean meal diet containing 3.0% soybean oil (SOY3.0). No differences were found for the brain total lipid content and FA profile between the different levels of soybean oil. For differential expression analysis, using the DESeq2 statistical package, a total of 34 differentially expressed genes (DEG, FDR-corrected p-value < 0.05) were identified. Of these 34 DEG, 25 are known-genes, of which 11 were up-regulated (log2 fold change ranging from + 0.25 to + 2.93) and 14 were down-regulated (log2 fold change ranging from − 3.43 to -0.36) for the SOY1.5 group compared to SOY3.0. For the functional enrichment analysis performed using MetaCore with the 34 DEG, four pathway maps were identified (p-value < 0.05), related to the ALOX15B (log2 fold change − 1.489), CALB1 (log2 fold change − 3.431) and CAST (log2 fold change + 0.421) genes. A “calcium transport” network (p-value = 2.303e-2), related to the CAST and CALB1 genes, was also identified. Conclusion The results found in this study contribute to understanding the pathways and networks associated with processes involved in intracellular calcium, lipid metabolism, and oxidative processes in the brain tissue. Moreover, these results may help a better comprehension of the modulating effects of soybean oil and its FA composition on processes and diseases affecting the brain tissue.

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“…org/Sus_scrofa/Info/Index]. The abundance (read counts) of mRNAs for all annotated genes was calculated using STAR-2.7.6a (Dobin and Gingeras, 2015;Fanalli et al, 2022a;2022b). Gene expression levels were normalized using counts scaled by the total number of reads (CPM-counts per million) using the R package DESeq2 (Love et al, 2014).…”

Section: Quality Control and Alignmentmentioning

confidence: 99%

RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids

et al. 2023

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Pigs (Sus scrofa) are an animal model for metabolic diseases in humans. Pork is an important source of fatty acids (FAs) in the human diet, as it is one of the most consumed meats worldwide. The effects of dietary inclusion of oils such as canola, fish, and soybean oils on pig gene expression are mostly unknown. Our objective was to evaluate FA composition, identify changes in gene expression in the liver of male pigs fed diets enriched with different FA profiles, and identify impacted metabolic pathways and gene networks to enlighten the biological mechanisms’ variation. Large White male pigs were randomly allocated to one of three diets with 18 pigs in each; all diets comprised a base of corn and soybean meal to which either 3% of soybean oil (SOY), 3% canola oil (CO), or 3% fish oil (FO) was added for a 98-day trial during the growing and finishing phases. RNA sequencing was performed on the liver samples of each animal by Illumina technology for differential gene expression analyses, using the R package DESeq2. The diets modified the FA profile, mainly in relation to polyunsaturated and saturated FAs. Comparing SOY vs. FO, 143 differentially expressed genes (DEGs) were identified as being associated with metabolism, metabolic and neurodegenerative disease pathways, inflammatory processes, and immune response networks. Comparing CO vs. SOY, 148 DEGs were identified, with pathways related to FA oxidation, regulation of lipid metabolism, and metabolic and neurodegenerative diseases. Our results help explain the behavior of genes with differential expression in metabolic pathways resulting from feeding different types of oils in pig diets.

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Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Cited by 7 publications

References 94 publications

Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Brain fatty acid and transcriptome profiles of pig fed diets with different levels of soybean oil

RNA-seq transcriptome profiling of pigs’ liver in response to diet with different sources of fatty acids

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