Differential Gene Expression Changes in Human Primary Dental Pulp Cells Treated with Biodentine and TheraCal LC Compared to MTA

Abstract

This study aimed to analyze the effects of pulp capping materials on gene expression changes in primary tooth-derived dental pulp cells using next-generation sequencing. Dental pulp cells were extracted and treated with mineral trioxide aggregate (MTA), Biodentine (BD), or TheraCal LC (TC). Cell viability assays were performed. Total RNA was extracted and analyzed through mRNA sequencing. Bioinformatic analysis of differential gene expression in dental pulp cells exposed to BD or TC versus MTA was performed. MTA, BD, and TC exposure had no significant effect on pulp cell viability (p > 0.05). Gene sets associated with inflammatory response (p = 2.94 × 10−5) and tumor necrosis factor alpha (TNF-α) signaling via the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway (p = 2.94 × 10−5) were enriched in all materials. In BD-treated cells, Wnt/β-catenin signaling (p = 3.15 × 10−4) gene sets were enriched, whereas enrichment of interferon gamma (IFN-γ) response (p = 3 × 10−3) was observed in TC-treated cells. In gene plot analysis, marked increases in receptor activator of nuclear factor kappa-Β ligand (RANKL) expression were seen in TC-treated cells over time. Despite the similar cell viabilities exhibited among MTA-, BD-, and TC-treated cells, patterns of gene networks differed, suggesting that diverse functional gene differences may be associated with treatment using these materials.

Evaluation of Pulp Repair after BiodentineTM Full Pulpotomy in a Rat Molar Model of Pulpitis

“…On the one hand, hydroxide ions create an alkaline environment which has an antibacterial and antiinflammatory effect, and on the other, Ca 2+ ions play a major role as intracellular second messengers that regulate a wide range of cellular processes, including gene transcription; protein expression; cell proliferation, differentiation and apoptosis; and excitatory cell activation [16,17]. Recently, Biodentine TM has been shown to induce the release of large numbers of Ca 2+ ions, this being reflected in altered intracellular Ca 2+ dynamics, and, in turn, differential gene expression, cellular differentiation and mineralization potential of human dental pulp stem cells (hDPSCs) stimulated with TCS-based cements [18,19].…”

Models for Oral Biology Research

“…Experiments with ferritin, a biomarker for iron storage, demonstrated the applicability of the selected model for transport studies, which is of great importance since cell line-based models of the epithelium of the submandibular salivary gland are still missing for this purpose. The aim of the study by Nam et al [12] was to analyze changes in gene expression in dental pulp cells of deciduous teeth, derived from the use of pulp capping materials. Dental pulp cells were extracted and treated with mineral trioxide aggregate (MTA), Biodentine (BD) or TheraCal LC (TC).…”