Differential Gene Expression Identified by Suppression Subtractive Hybridization During Late Ripening of Fruit in Oil Palm (Elaeis guineensis Jacq.)

Al-Shanfari, Ahmed Bakhit; Abdullah, Siti Nor Akmar; Saud, Halimi Mohd; Omidvar, Vahid; Napis, Suhami

doi:10.1007/s11105-011-0383-5

Cited by 4 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, different scales of the EST sequencing project identifying 10, 50, 37.6, and 6 % as sequences encoding MT from the banana, pineapple, oil palm, and citrus fruit cDNA library, respectively (Liu et al 2002, Moyle et al 2005, Low et al 2008, Ye et al 2010. In our previous study, also two different partial cDNA sequences encoding type 2 MT proteins designated as MET2a and MET2b occurred at a relatively high abundance (16.05 %) at the late ripening stage of the oil palm fruit (Al-Shanfari et al 2012). Sequence analysis revealed that these cDNAs encode small polypeptides with Cys-rich N-and C-terminal domains which are the common features of plant MT and MT-like proteins (Robinson et al 1993).…”

Section: Discussionmentioning

confidence: 88%

“…In the present study, the expression profiles of four MT genes whose transcripts have been found in oil palm fruits were compared. The genes were MT3-A and MT3-B, previously reported by Abdullah et al 2002, and MET2a and MET2b which were reported in our previous study (Al-Shanfari et al 2012). The objectives of this study were to obtain and characterize the full-length cDNA clones of MET2a and MET2b from oil palm and to compare the expression profiles of the four different MT genes at different stages of development in the fruit mesocarp and in vegetative tissues.…”

Section: Introductionmentioning

confidence: 75%

See 1 more Smart Citation

Isolation and expression analysis of transcripts encoding metallothioneins in oil palm

Al-Shanfari¹,

Abdullah²

2014

Biologia plant.

View full text Add to dashboard Cite

Two of the abundant transcripts encoding type 2 metallothionein (MT) proteins designated as MET2a and MET2b were selected in our previous study due to their high abundance (16.05 %) in the suppression subtractive hybridization library and their involvement in fruit development and maturation. The present study involves the isolation of the full-length cDNA encoding MET2a and MET2b from the ripening oil palm fruit mesocarp, examining their expression pattern compared to the other two previously reported type-3 MT members (MT3-A and MT3-B) in various oil palm organs including different vegetative and reproductive tissues. The full-length cDNA sequences of MET2a and MET2b were 571 and 553 bp and they were designated as EgMT2a and EgMT2b, respectively. The sequences of the EgMT2a and EgMT2b were then compared for sequence similarities in the database using both BLASTN and BLASTX programs. Their sequences were homologous (67 -77 %) with several type-2 MTs in plants. All four MT encoding genes were differentially expressed in the ripening oil palm mesocarp tissues, but undetectable in the vegetative tissues examined. All MT genes examined were significantly up-regulated in the mature developmental stages of oil palm fruit mesocarp, except for EgMT2b which was expressed only at 17 weeks after anthesis. The type 2 MT proteins are related to a greater degree to the late fruit-ripening stage than the type 3 MT proteins consistent with their reported functions in homeostasis or detoxification. The findings in the present study contribute to better understanding the molecular mechanisms involved in fruit ripening in oil palm.

show abstract