Differential gene expression in oyster exposed to sewage

Abstract

In order to investigate the influence of domestic sewage on the gene expression of Pacific oysters Crassostrea gigas, suppression subtractive hybridization (SSH) method was employed. Oysters were sampled at a farming area and, after 10 days of acclimation in the laboratory, were exposed to untreated domestic sewage diluted 33% for 48 h. Gills of male oysters were excised for total RNA extraction. mRNA was purified and the differential gene expression was analyzed by SSH. We obtained 61 cDNA sequences but only 15 were identified, which includes fatty acid binding protein, multidrug resistance protein, omega glutathione S-transferase, cytochrome P450 isoform CYP356A1, among others. The identified genes are associated with different metabolic functions like biotransformation, membrane transport, aerobic metabolism and translational machinery, evidencing the potential toxic effects elicited by these effluents.

Effect of linear alkylbenzene mixtures and sanitary sewage in biochemical and molecular responses in pacific oyster Crassostrea gigas

“…Previous studies have shown that CYP356A1 mRNA is highly induced in oyster exposed to sanitary sewage under laboratory conditions (Medeiros et al 2008a). However, in the present study, lower levels of CYP356A1 mRNA were observed in digestive gland of oysters exposed for 12 h to LAB (Fig.…”

“…The raw sanitary sewage was collected at the entrance of the Insular Wastewater Treatment Plant (CASAN, Florianópolis, SC, Brazil) and used according to the methodology described by Medeiros et al (2008a). The LAB nominal concentration used was chosen based on the levels of these mixtures accumulated in oyster C. gigas kept in a site contaminated by sanitary sewage discharges (Souza et al 2012).…”

“…Recent studies using biomarkers have shown the impact of sanitary sewage on marine organisms (Andrew-Priestley et al 2012;Comoglio et al 2011;Marigómez et al 2013;Medeiros et al 2008a;Zanette et al 2008). Analysis of transcriptional level of genes that encode proteins with important roles in cellular metabolism and biotransformation (e.g., SOD, CAT, G6PDH, GSTs, HSP70) can help to elucidate the actual impact caused by contaminants in sentinel organisms (Medeiros et al 2008b).…”

Alternative oxidase in animals: unique characteristics and taxonomic distribution

“…Prior work in C. gigas detected AOX transcripts in the gill, heart, adductor muscle, hemolymph and mantle tissues (McDonald and Vanlerberghe, 2004). EST data indicate that AOX is detected in C. gigas exposed to sewage or bacterial challenge (Medeiros et al, 2008;Roberts et al, 2009) (see Table S1 in supplementary material). RT-PCR using degenerate animal AOX primers detected AOX transcript in C. virginica adductor muscle (see Fig.…”

Understanding the role of ecological indicator use in assessing the effects of desalination plants

“…These results suggest that biochemical studies of sea grass ecosystem damage by a desalination project as a result of ecological indicators can be used in same way as a coral reef ecosystem in the future. To investigate the influence of sewage on the differential gene expression of Pacific oysters, Crassostrea gigas, suppression subtractive hybridization method was used [145]. This research examined a desalination indicator gene; also, the genes identified were associated with different metabolic functions, like biotransformation, membrane transport, and aerobic metabolism.…”