Differential Gene Expression Pattern of Drought Responsive Transcription Factors in Chickpea: An Expressional Analysis

Borhani, Sara; Vessal, Saeedreza; Bagheri, Abdolreza; Shokouhifar, Farhad

doi:10.1007/s00344-019-10056-5

Cited by 3 publications

(1 citation statement)

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“…However, for functional analysis, it is important to show how gene expression is changed in response to stress treatment or during plant development. RT-qPCR or RNA-seq technology thus becomes a reasonable requirement for gene studies in plants (Upton et al, 2023), including chickpea (Nguyen et al, 2015;Borhani et al, 2020). Based on genotyping and gene expression analysis, the final step for "proof of a hypothesis" is to show how selected genotypes are more tolerant to stresses and how seed yield and quality are improved.…”

Section: Introductionmentioning

confidence: 99%

Zinc finger knuckle genes are associated with tolerance to drought and dehydration in chickpea (Cicer arietinum L.)

Khassanova,

Oshergina,

Ten

et al. 2024

Front. Plant Sci.

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Chickpea (Cicer arietinum L.) is a very important food legume and needs improved drought tolerance for higher seed production in dry environments. The aim of this study was to determine diversity and genetic polymorphism in zinc finger knuckle genes with CCHC domains and their functional analysis for practical improvement of chickpea breeding. Two CaZF-CCHC genes, Ca04468 and Ca07571, were identified as potentially important candidates associated with plant responses to drought and dehydration. To study these genes, various methods were used including Sanger sequencing, DArT (Diversity array technology) and molecular markers for plant genotyping, gene expression analysis using RT-qPCR, and associations with seed-related traits in chickpea plants grown in field trials. These genes were studied for genetic polymorphism among a set of chickpea accessions, and one SNP was selected for further study from four identified SNPs between the promoter regions of each of the two genes. Molecular markers were developed for the SNP and verified using the ASQ and CAPS methods. Genotyping of parents and selected breeding lines from two hybrid populations, and SNP positions on chromosomes with haplotype identification, were confirmed using DArT microarray analysis. Differential expression profiles were identified in the parents and the hybrid populations under gradual drought and rapid dehydration. The SNP-based genotypes were differentially associated with seed weight per plant but not with 100 seed weight. The two developed and verified SNP molecular markers for both genes, Ca04468 and Ca07571, respectively, could be used for marker-assisted selection in novel chickpea cultivars with improved tolerance to drought and dehydration.

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Section: Introductionmentioning

confidence: 99%