Differential gene expression profile from haematopoietic tissue stem cells of red claw crayfish, Cherax quadricarinatus, in response to WSSV infection

Liu, Haipeng; Chen, Rongyuan; Zhang, Qiuxia; Peng, Hui; Wang, Kejian

doi:10.1016/j.dci.2011.02.015

Cited by 68 publications

(21 citation statements)

References 61 publications

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“…The transcriptional profile of WSSV genes in shrimp was detected by DNA microarray and some early genes were discovered [2]. Many host genes and proteins responding to WSSV infection were also identified through large scale approaches [3–7]. From these studies, a lot of host genes and proteins were found upregulated or downregulated after WSSV infection.…”

Section: Introductionmentioning

confidence: 99%

Bioinformatic Prediction of WSSV-Host Protein-Protein Interaction

Sun

et al. 2014

BioMed Research International

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WSSV is one of the most dangerous pathogens in shrimp aquaculture. However, the molecular mechanism of how WSSV interacts with shrimp is still not very clear. In the present study, bioinformatic approaches were used to predict interactions between proteins from WSSV and shrimp. The genome data of WSSV (NC_003225.1) and the constructed transcriptome data of F. chinensis were used to screen potentially interacting proteins by searching in protein interaction databases, including STRING, Reactome, and DIP. Forty-four pairs of proteins were suggested to have interactions between WSSV and the shrimp. Gene ontology analysis revealed that 6 pairs of these interacting proteins were classified into “extracellular region” or “receptor complex” GO-terms. KEGG pathway analysis showed that they were involved in the “ECM-receptor interaction pathway.” In the 6 pairs of interacting proteins, an envelope protein called “collagen-like protein” (WSSV-CLP) encoded by an early virus gene “wsv001” in WSSV interacted with 6 deduced proteins from the shrimp, including three integrin alpha (ITGA), two integrin beta (ITGB), and one syndecan (SDC). Sequence analysis on WSSV-CLP, ITGA, ITGB, and SDC revealed that they possessed the sequence features for protein-protein interactions. This study might provide new insights into the interaction mechanisms between WSSV and shrimp.

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Section: Introductionmentioning

confidence: 99%

Bioinformatic Prediction of WSSV-Host Protein-Protein Interaction

Sun

et al. 2014

BioMed Research International

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“…The Unclassified identification and characterization of genes involved in immune response have facilitated the study of expression of genes during the disease processes, which help in better understanding of the mechanisms underlying disease resistance/susceptibility. The altered expression of different cytokine and immune-related genes has been observed following infection (Liu et al, 2011;Mohanty and Sahoo, 2010;Saurabh et al, 2011), vaccination (Gioacchini et al, 2008;Jorgensen et al, 2008) and immuno-stimulation (Watanuki et al, 2006).…”

Section: Resultsmentioning

confidence: 95%

EST-based identification of immune-relevant genes from spleen of Indian catfish, Clarias batrachus (Linnaeus, 1758)

Singh

Sood

Chauhan

et al. 2012

Gene

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“…To get insight into the proteins differentially expressed at both early stage (1 hpi) and late stage (12 hpi) of WSSV infection as recent publications reported that these two time points have been clearly defined accordingly of WSSV replication in crayfish hpt cells [12,13], iTRAQ analysis was employed for quantification followed by protein profiling, which resulted in 594 protein hits in Mascot. The functional enrichment analysis of GO annotations for biological processes showed that the identified proteins were classified into 23 categories ( Fig.…”

Section: Protein Profiling and Itraq Quantificationmentioning

confidence: 99%

“…Previously, we have investigated the transcript alteration of Hpt cells from red claw crayfish, Cherax quadricarinatus, towards WSSV challenge using subtractive library screening. We noticed that numerous genes, with differential expression between WSSV infection and mocked infection, were present with annotated functions in immune defense, cytoskeletal organization, signal transduction, stress, metabolism and homeostasis [13]. To further identify proteins or pathways altered during viral infection, here we report proteomic responses of crayfish Hpt cells by iTRAQ at both early (1 hpi) and late (12 hpi) stages post WSSV infection accordingly.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis by iTRAQ in red claw crayfish, Cherax quadricarinatus, hematopoietic tissue cells post white spot syndrome virus infection

Jeswin

Xie

et al. 2016

Fish & Shellfish Immunology

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To elucidate proteomic changes of Hpt cells from red claw crayfish, Cherax quadricarinatus, we have carried out isobaric tags for relative and absolute quantitation (iTRAQ) of cellular proteins at both early (1 hpi) and late stage (12 hpi) post white spot syndrome virus (WSSV) infection. Protein database search revealed 594 protein hits by Mascot, in which 17 and 30 proteins were present as differentially expressed proteins at early and late viral infection, respectively. Generally, these differentially expressed proteins include: 1) the metabolic process related proteins in glycolysis and glucogenesis, DNA replication, nucleotide/amino acid/fatty acid metabolism and protein biosynthesis; 2) the signal transduction related proteins like small GTPases, G-protein-alpha stimulatory subunit, proteins bearing PDZ- or 14-3-3-domains that help holding together and organize signaling complexes, casein kinase I and proteins of the MAP-kinase signal transduction pathway; 3) the immune defense related proteins such as α-2 macroglobulin, transglutaminase and trans-activation response RNA-binding protein 1. Taken together, these protein information shed new light on the host cellular response against WSSV infection in a crustacean cell culture.

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Differential gene expression profile from haematopoietic tissue stem cells of red claw crayfish, Cherax quadricarinatus, in response to WSSV infection

Cited by 68 publications

References 61 publications

Bioinformatic Prediction of WSSV-Host Protein-Protein Interaction

Bioinformatic Prediction of WSSV-Host Protein-Protein Interaction

EST-based identification of immune-relevant genes from spleen of Indian catfish, Clarias batrachus (Linnaeus, 1758)

Proteomic analysis by iTRAQ in red claw crayfish, Cherax quadricarinatus, hematopoietic tissue cells post white spot syndrome virus infection

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