Differential microRNA expression profile in the plasma of preeclampsia and normal pregnancies

Abstract

Preeclampsia is a common disease in pregnant women that can only be diagnosed from 20 weeks after fertilization. Developing early diagnosis markers is urgent and would be helpful in selecting appropriate treatment strategies. The present study aimed to identify the differential expression profiles of microRNAs in the plasma between patients with preeclampsia and normal pregnancies using microarray methods. Using quantitative polymerase chain reaction (qPCR), the differentially expressed microRNAs (miRNAs or miRs) identified from the microarray analysis were validated. A total of 3 miRNAs, including hsa-miR-1304-5p, hsa-miR-320a and hsa-miR-5002-5p, were upregulated in the plasma of patients with preeclampsia pregnancies. Examination of the functions of these miRNAs demonstrated that they were involved in cell proliferation, indicating that preeclampsia affected this pathway. In addition, 26 downregulated miRNAs were identified by microarray methods. The functions of these miRNAs included immune regulation, vascular development, cancer pathology and pathology of other disease (tuberculosis, oligozoospermia, psoriasis and Alzheimer's disease). Using qPCR, the most differentially expressed miRNAs were confirmed to be hsa-miR-1304-5p, hsa-miR-320a and hsa-miR-5002-5p, which were upregulated, as well as hsa-miR-188-3p, hsa-miR-211-5p, hiv1-miR-TAR-3p, hsa-miR-4432 and hsa-miR-4498 that were significantly downregulated in the plasma of preeclampsia patients. The present findings may be useful in the development of early diagnosis markers and treatment targets for preeclampsia.

Bioinformatics Analysis of Evolution and Human Disease Related Transposable Element-Derived microRNAs

“…miRNA recognizes the complementary binding sites of target gene for its seed regions which is approximately 6 nucleotides long in miRNA. Previously, numerous studies have provided the evidence of identification on miRNA derived from TE [30,31,33,34,36] and some of the miRNAs derived from TE had a strong correlation with human disease [46][47][48][49][50][51][52] as well as evolution [53][54][55][56][57]. Considering the many substantial aspects of TE, miRNAs derived from TE mimic the functions of TE.…”

Clinical significance of microRNA-320a and insulin-like growth factor-1 receptor in early-onset preeclampsia patients

“…We also identi ed that miR-320a had potent clinical diagnostic value for EOPE using ROC curve, as con rmed by an area under ROC curve of 0.790, a speci city of 71.40% and a sensitivity of 70.00%. In support of these, a previous study has provided evidence that miR-320a is abnormally enhanced in PE patients, promising to be a therapeutic target for PE therapy [22]. It is well established that increased urinary protein excretion, MAP, UA and SCR levels during pregnancy are potent indexes for EOPE prediction [23][24][25][26].…”

Insight into the Key Points of Preeclampsia Pathophysiology: Uterine Artery Remodeling and the Role of MicroRNAs

“…The five most downregulated miRNAs were hsa-miRNA-188-3p, hsa-miRNA-211-5p, hiv1-miRNA-TAR-3p, hsa-miRNA-4432, and hsa-miRNA-4498. Functional analysis of differentially expressed miRNA in this study revealed that upregulated miRNAs were involved in the cell proliferation and migration, whereas downregulated miRNA in immune regulation, vascular development, and cancer pathology (cell cycle progression) [ 88 ]. Similar analysis was performed by Lip et al, but it concerned early-onset PE.…”