Differential Migration and Proliferation Potential of the Hydrogel Aided 3D Tumoroid

Abstract

For substantial in vitro cancer biology research, the 3D cell culture method has now been regarded as more suitable model expected to be recapitulating maximum in vivo tumor mass relevance. Despite of available techniques to develop in vitro 3D models, a system availing a physiologically relevant in vitro 3D model of primary lung adenocarcinoma with extracellular matrix (ECM) mimicry and similar tumorigenic properties still remains a quest. Thus, in the present study, chemically modified Dextran-Chitosan (MDC) hydrogel has been developed as a 3D tumoroid aiding scaffold. The 3D A549 tumoroids aided by the MDC scaffold have physiologically relevant proliferation, migration, invasive potential, and Gefitinib [targeting epidermal growth factor receptor (EGFR)] efficacy as compared to the 2D cultured cells. The surface topography and wettability of hydrogel availed in vivo micro tumor mass mimicking Lung adenocarcinoma 3D in vitro model. Thus, opening an innovative avenue for elucidating the disease mechanism and drug efficacy on relevant 3D cancer models in vitro.

Site-selective post-modification of short α/γ hybrid foldamers: a powerful approach for molecular diversification towards biomedical applications

“…64 The biocompatibility of the peptides, PNs , and PNDs was determined in vitro in triple-negative breast cancer cells (MDA-MB-231) by MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) assay. 65 Peptide 1 showed a gradual decrease in cell viability with increasing peptide concentration (IC 50 = 80 μM), whereas peptides 2 and 4 showed significant cell death even at low concentrations (IC 50 = 1 μM). Peptide 1 with its better solubility in comparison with O -alkylated peptides 2 and 4 might have enhanced its bioavailability which could be a possible reason for its improved biocompatibility.…”

Folate conjugated albumin as a targeted nanocarrier for the delivery of fisetin: in silico and in vitro biological studies

“…Cell migration plays a crucial role in tumor progression and metastasis. 52 The impact of FST, FNPs, and FFANPs on cell migration was assessed through a scratch assay. In this assay, cells were seeded in 12-well plates and allowed to form a confluent monolayer.…”

Folate Functionalized and Evodiamine‐Loaded Pluronic Nanomicelles for Augmented Cervical Cancer Cell Killing

“…In Vitro Wound Healing Assay: Wound healing assay was performed to examine cell migratory response after treatment with ENM as per the previously reported study. [71] HeLa cells were grown in 6-well culture plate with 1.0 × 10 5 cells per well density and cells were incubated till full confluency. HeLa cell monolayer was scratched with a micropipette tip (200 μL) once they had attained full confluency, and cells were then washed in PBS.…”