Differential miRNA Expression Profiling Reveals Correlation of miR125b-5p with Persistent Infection of Japanese Encephalitis Virus

Huang, Chih-Wei; Tsai, Kuen-Nan; Chen, Yi-Shiuan; Chang, Ruey-Yi

doi:10.3390/ijms22084218

Cited by 15 publications

(9 citation statements)

References 66 publications

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“…This may be because the dysregulated miRNAs are involved in the regulation of inflammation and viral response in patients. It was shown that miR-125b-5p targets STAT3 in Japanese encephalitis virus infection, thereby coordinating viral replication and host antiviral response and that when the miR-125b-5p expression was upregulated, the expression levels of target genes in persistently infected cells were both decreased, 40 it is consistent with the expression of miR-125b-5p and STAT3 in this study. STAT3 regulates a variety of biological functions and plays a key role in many cellular processes including cell growth and apoptosis, STAT3 can be up- or down-regulated in viral infections, depending on the type of virus involved.…”

Section: Discussionsupporting

confidence: 86%

Potential Predictive Value of miR-125b-5p, miR-155-5p and Their Target Genes in the Course of COVID-19

Li¹,

Wang²,

Zhou³

et al. 2022

IDR

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Purpose This study aimed to provide new biomarkers for predicting the disease course of COVID-19 by analyzing the dynamic changes of microRNA (miRNA) and its target gene expression in the serum of COVID-19 patients at different stages. Methods Serum samples were collected from all COVID-19 patients at three time points: the acute stage, the turn-negative stage, and the recovery stage. The expression level of miRNA and the target mRNA was measured by Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR). The classification tree model was established to predict the disease course, and the prediction efficiency of independent variables in the model was analyzed using the receiver operating characteristic (ROC) curve. Results The expression of miR-125b-5p and miR-155-5p was significantly up-regulated in the acute stage and gradually decreased in the turn-negative and recovery stages. The expression of the target genes CDH5, STAT3, and TRIM32 gradually down-regulated in the acute, turn-negative, and recovery stages. MiR-125b-5p, miR-155-5p, STAT3, and TRIM32 constituted a classification tree model with 100% accuracy of prediction and AUC >0.7 for identification and prediction in all stages. Conclusion MiR-125b-5p, miR-155-5p, STAT3, and TRIM32 could be useful biomarkers to predict the time nodes of the acute, turn-negative, and recovery stages of COVID-19.

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Section: Discussionsupporting

confidence: 86%

Potential Predictive Value of miR-125b-5p, miR-155-5p and Their Target Genes in the Course of COVID-19

Li¹,

Wang²,

Zhou³

et al. 2022

IDR

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“…Persistently JEV infection increases miR-125b-5p expression and limits viral replication by dampening STAT3, MAP2K7, TRIAP1 production, which genes are associated with apoptosis and cell growth ( Huang et al, 2021 ). Overexpression of miR-124 in PK-15 cells is found to decrease the expression level of DNM2, a GTPase responsible for vesicle scission, further impairing JEV proliferation ( Yang et al, 2016 ).…”

Section: Regulation Of Mirnas In Japanese Encephalitis Virus Infectionmentioning

confidence: 99%

Regulatory Role of Host MicroRNAs in Flaviviruses Infection

et al. 2022

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MicroRNAs (miRNAs) are small non-coding RNA that affect mRNA abundance or translation efficiency by binding to the 3′UTR of the mRNA of the target gene, thereby participating in multiple biological processes, including viral infection. Flavivirus genus consists of small, positive-stranded, single-stranded RNA viruses transmitted by arthropods, especially mosquitoes and ticks. The genus contains several globally significant human/animal pathogens, such as Dengue virus, Japanese encephalitis virus, West Nile virus, Zika virus, Yellow fever virus, Tick-borne encephalitis virus, and Tembusu virus. After flavivirus invades, the expression of host miRNA changes, exerting the immune escape mechanism to create an environment conducive to its survival, and the altered miRNA in turn affects the life cycle of the virus. Accumulated evidence suggests that host miRNAs influence flavivirus replication and host–virus interactions through direct binding of viral genomes or through virus-mediated host transcriptome changes. Furthermore, miRNA can also interweave with other non-coding RNAs, such as long non-coding RNA and circular RNA, to form an interaction network to regulate viral replication. A variety of non-coding RNAs produced by the virus itself exert similar function by interacting with cellular RNA and viral RNA. Understanding the interaction sites between non-coding RNA, especially miRNA, and virus/host genes will help us to find targets for antiviral drugs and viral therapy.

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“…However, little is known about the effects miRNA expression may have on flavivirus-persistent infection. A miRNA array analysis of Japanese encephalitis virus (JEV)-infected cells was performed to search for persistent infection-associated miRNAs in comparison to acute infection [ 178 ]. Among all differentially expressed miRNAs, the miR-125b-5p is most significantly increased [ 178 ].…”

Section: Mechanisms Of Flavivirus Persistencementioning

confidence: 99%

Flavivirus Persistence in Wildlife Populations

Blahove

Carter

2021

Viruses

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A substantial number of humans are at risk for infection by vector-borne flaviviruses, resulting in considerable morbidity and mortality worldwide. These viruses also infect wildlife at a considerable rate, persistently cycling between ticks/mosquitoes and small mammals and reptiles and non-human primates and humans. Substantially increasing evidence of viral persistence in wildlife continues to be reported. In addition to in humans, viral persistence has been shown to establish in mammalian, reptile, arachnid, and mosquito systems, as well as insect cell lines. Although a considerable amount of research has centered on the potential roles of defective virus particles, autophagy and/or apoptosis-induced evasion of the immune response, and the precise mechanism of these features in flavivirus persistence have yet to be elucidated. In this review, we present findings that aid in understanding how vector-borne flavivirus persistence is established in wildlife. Research studies to be discussed include determining the critical roles universal flavivirus non-structural proteins played in flaviviral persistence, the advancement of animal models of viral persistence, and studying host factors that allow vector-borne flavivirus replication without destructive effects on infected cells. These findings underscore the viral–host relationships in wildlife animals and could be used to elucidate the underlying mechanisms responsible for the establishment of viral persistence in these animals.

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Differential miRNA Expression Profiling Reveals Correlation of miR125b-5p with Persistent Infection of Japanese Encephalitis Virus

Cited by 15 publications

References 66 publications

Potential Predictive Value of miR-125b-5p, miR-155-5p and Their Target Genes in the Course of COVID-19

Potential Predictive Value of miR-125b-5p, miR-155-5p and Their Target Genes in the Course of COVID-19

Regulatory Role of Host MicroRNAs in Flaviviruses Infection

Flavivirus Persistence in Wildlife Populations

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