Differential PDGF secretion by graft and aortic SMC in response to oxidized LDL

Abstract

Absood, Afaf, Akira Furutani, Tsutomu Kawamura, and Linda M. Graham. Differential PDGF secretion by graft and aortic SMC in response to oxidized LDL.

A comparison of oxidized LDL-induced collagen secretion by graft and aortic SMCs: role of PDGF

“…Cells were harvested from the proximal aorta (ascending aorta and aortic arch), the distal abdominal aorta below the graft anastomosis, and sections of the midgraft as previously described (1). Briefly, the luminal surface was incubated in collagenase A (630 U/ml, Boehringer Mannheim Biochemicals) for 10 min at 37°C, and endothelial cells were removed mechanically.…”

“…Purified LDL was filtered through a 0.45-m filter. LDL was stored in the dark at 4°C under nitrogen and oxidized with 1.5 M copper sulfate as previously described (1). The level of LDL oxidation was monitored by the formation of thiobarbituric acid-reacting substances (TBARS), measured by the method of Schuh and colleagues (40).…”

“…These phenotypic differences between graft and aortic SMCs are maintained for several passages in culture. Because oxLDL stimulates PDGF production by graft SMCs (1), and PDGF stimulates collagen production under some circumstances, we studied the ability of oxLDL to stimulate collagen production by graft and aortic SMCs and the role of PDGF in this stimulation. Graft SMCs not only produced more collagen than aortic SMCs under basal conditions but also the graft SMCs responded to oxLDL and a superoxide generator, LY-83583, with a larger increase in collagen production than aortic SMCs.…”

Established and Emerging Plasma Biomarkers in the Prediction of First Atherothrombotic Events

“…133 Ox-LDL also activates inflammatory cells and facilitates release of a number of growth factors from monocytes/macrophages. 134,135 Vascular smooth muscle cells exhibit intense proliferation when exposed to ox-LDL. 136 Platelet eNOS activity is diminished in the presence of ox-LDL, and these cells demonstrate intense activation when exposed to small amounts of thrombin.…”

Oxidized or Native Low-Density Lipoprotein Cholesterol⁎Editorials published in the Journal of American College of Cardiologyreflect the views of the authors and do not necessarily represent the views of JACCor the American College of Cardiology.

“…The ox-LDL decreases the gene expression of endothelial nitric oxide synthase (eNOS) and enhances generation of reactive oxygen species (17). The ox-LDL itself activates inflammatory cells and facilitates release of growth factors from monocytes/macrophages (18,19). Platelet eNOS activity is diminished in the presence of ox-LDL, and these cells demonstrate intense activation in response to small amounts of thrombin (20).…”