Differential physiological and biochemical responses under variable culture conditions in micro-propagated Solenostemon scutellarioides: an important ornamental plant

Abstract

Abstract:Solenostemon scutellarioides is a commercially important ornamental plant. In present study, it was attempted to establish a protocol for high frequency in vitro regeneration of S. scutellarioides. Caulogenesis was found to be significant in solid MS medium supplemented with 0.5 mg dm -3 BAP. Combination of GA 3 (0.5 mg dm -3 ) and BAP (0.5 mg dm -3 ) induced high frequency regeneration coupled with higher plant height. The plantlets exposed to IBA (1 mg dm -3 ) exhibited significant root development in terms of first appearance of root and number of roots per shoot. To improve its commercial acceptability in terms of plant architecture and foliage colouration, two weeks old in vitro grown plantlets were exposed to different culture conditions namely MS strength, sucrose concentration, pH and light. A variable plant types with diverse ornamental traits were developed under different culture conditions.

Does the presence of leaves on coleus stem cuttings influence their rooting?

“…IBA is a plant growth regulator widely used to stimulate stem cuttings rhizogenesis when endogen auxin production is insufficient for rooting and roots development (FRAGOSO et al, 2017). In coleus micropropagation, similarly, nodal fragments treated with IBA presented early rooting and an increase in the number of roots per propagule (SAHU and DEWANJEE, 2012).…”

“…Br., whose scientific synonyms are Coleus blumei Benth. (SYTAR et al, 2015) and Solenostemon scutellarioides (L.) Codd (YOKO et al, 2015), is popularly known as coleus (DA-TILES and ACEVEDO-RODRÍGUEZ, 2014).…”

“…Coleus is characterized as perennial and vigorous foliage with upright, semi-upright, prostrate, semi-trailing, and trailing habits of growth (NGUYEN et al, 2008). It has been widely used as flowerbeds in landscaping gardening projects and has gained importance in the floriculture industry with the introduction of a large number of vegetatively propagated varieties with novel foliage colors and shapes (SAHU and DEWANJEE, 2012).…”

Bioproduction and Optimization of Rosmarinic Acid Production in Solenostemon Scutellarioides through Media Manipulation and Conservation of High Yielding Clone via Encapsulation

“…After each storage period, encapsulated and non-encapsulated nodal segments were placed onto semi-solid MS medium for regeneration. To test efficiency of regeneration potential, buds were taken out by rupturing the bead matrix and allowed to culture using the established regeneration protocol [4]. The plants were grown for 8 weeks to determine regeneration percentage, fresh biomass (g FW ) and RA content.…”

“…), a commercially important ornamental plant, contains a significant quantity of RA. In our previous report, a high frequency regeneration protocol of S. scutellarioides through a clonal propagation technique was established [4]. The level of RA accumulation has been found to be dependent on different media parameters, such as plant growth regulators and sucrose concentrations in callus and cell suspension culture of S. scutellarioides [5][6][7].…”