Differential Proteomic Analysis of Human Glioblastoma and Neural Stem Cells Reveals HDGF as a Novel Angiogenic Secreted Factor

Thirant, Cécile; Moya, Eva M; Dubois, Luiz Gustavo; Pinte, Sébastien; Chafey, Philippe; Broussard, Cédric; Varlet, Pascale; Devaux, Bertrand; Soncin, Fabrice; Gavard, Julie; Junier, Marie‐Pierre; Chneiweiss, Hervé

doi:10.1002/stem.1062

Cited by 71 publications

(74 citation statements)

References 81 publications

(99 reference statements)

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“…Stem cells secrete neurotrophins and growth factors, some of which have been recently described to play a major role in stem cell-immune cell interactions (Ranganath et al, 2012;Thirant et al, 2012).…”

Section: Paracrine Signalingmentioning

confidence: 99%

How stem cells speak with host immune cells in inflammatory brain diseases

Pluchino

Cossetti

2013

Glia

111

109

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Advances in stem cell biology have raised great expectations that diseases and injuries of the central nervous system (CNS) may be ameliorated by the development of non-hematopoietic stem cell medicines. Yet, the application of adult stem cells as CNS therapeutics is challenging and the interpretation of some of the outcomes ambiguous. In fact, the initial idea that stem cell transplants work only via structural cell replacement has been challenged by the observation of consistent cellular signaling between the graft and the host. Cellular signaling is the foundation of coordinated actions and flexible responses, and arises via networks of exchanging and interacting molecules that transmit patterns of information between cells. Sustained stem cell graft-to-host communication leads to remarkable trophic effects on endogenous brain cells and beneficial modulatory actions on innate and adaptive immune responses in vivo, ultimately promoting the healing of the injured CNS. Among a number of adult stem cell types, mesenchymal stem cells (MSCs) and neural stem/precursor cells (NPCs) are being extensively investigated for their ability to signal to the immune system upon transplantation in experimental CNS diseases. Here, we focus on the main cellular signaling pathways that grafted MSCs and NPCs use to establish a therapeutically relevant cross talk with host immune cells, while examining the role of inflammation in regulating some of the bidirectionality of these communications. We propose that the identification of the players involved in stem cell signaling might contribute to the development of innovative, high clinical impact therapeutics for inflammatory CNS diseases.

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Section: Paracrine Signalingmentioning

confidence: 99%

How stem cells speak with host immune cells in inflammatory brain diseases

Pluchino

Cossetti

2013

Glia

111

109

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“…One candidate is hepatocyte growth factor (HGF), an angiogenic factor (Okada et al 1999) that induces HDGF expression in cancer cells (Mao et al 2008, Lee et al 2010. In fact, the downstream effects of HGF may be mediated in part by HDGF induction of vascular endothelial growth factor (Mao et al 2008, Lee et al 2010) and angiogenesis via paracrine mechanisms (Sasaki et al 2011, Thirant et al 2012, Tsai et al 2013.…”

Section: Discussionmentioning

confidence: 99%

“…vascular smooth muscle and endothelial and fibroblast cells; Klagsbrun et al 1986, including cancer cell lines (Bernard et al 2003, Hu et al 2003, Chang et al 2007, Lee et al 2010, Liao et al 2010, Yang et al 2013. In tumor cells, overexpressed HDGF increases proliferation, migration, and neoangiogenesis (Sasaki et al 2011, Thirant et al 2012, Tsai et al 2013, and it inhibits apoptosis (Lee et al 2010, Liao et al 2010.…”

Section: Introductionmentioning

confidence: 99%

Hepatoma-derived growth factor: from the bovine uterus to the in vitro embryo culture

Gómez¹,

Correia-Álvarez²,

Caamaño³

et al. 2014

Reproduction

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Early in cow embryo development, hepatoma-derived growth factor (HDGF) is detectable in uterine fluid. The origin of HDGF in maternal tissues is unknown, as is the effect of the induction on developing embryos. Herein, we analyze HDGF expression in day 8 endometrium exposed to embryos, as well as the effects of recombinant HDGF (rHDGF) on embryo growth. Exposure to embryos did not alter endometrial levels of HDGF mRNA or protein. HDGF protein localized to cell nuclei in the luminal epithelium and superficial glands and to the apical cytoplasm in deep glands. After uterine passage, levels of embryonic HDGF mRNA decreased and HDGF protein was detected only in the trophectoderm. In fetal fibroblast cultures, addition of rHDGF promoted cell proliferation. In experiments with group cultures of morulae in protein-free medium containing polyvinyl alcohol, adding rHDGF inhibited blastocyst development and did not affect cell counts when the morulae were early (day 5), whereas it enhanced blastocyst development and increased cell counts when the morulae were compact (day 6). In cultures of individual day 6 morulae, adding rHDGF promoted blastocyst development and increased cell counts. Our experiments with rHDGF indicate that the growth factor stimulates embryonic development and cell proliferation. HDGF is synthesized similarly by the endometrium and embryo, and it may exert embryotropic effects by autocrine and/or paracrine mechanisms.

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“…Cependant, le VEGF est loin d'être le seul facteur angiogénique sécrété par les CSC. Celles-ci expriment également des cytokines et divers facteurs de croissance dont le HDGF (hepatoma-derived growth factor) impliqué dans la migration des cellules endothéliales cérébrales [17]. Par ailleurs, les CSC sécrètent de la sémaphorine 3A (Sema3A), qui augmente la perméabilité endothéliale en perturbant la stabilité de la VE-cadhérine et l'organisation des jonctions cellule-cellule [18].…”

Section: Les Cellules Tumorales à Caractère Soucheunclassified

Vaisseaux sanguins et tumeurs ou l’art du dialogue

Azzi

Gavard

2014

Med Sci (Paris)

Self Cite

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> Le réseau vasculaire joue un rôle essentiel dans le développement des cancers. Au cours de la dernière décennie, la découverte de cellules tumorales à caractère souche logées à proximité des vaisseaux sanguins a permis de mettre en lumière l'existence d'interactions fonctionnelles entre l'endothélium et les cellules tumorales. Dans cette revue, nous présentons les mécanismes d'angiogenèse, ainsi que les éléments de langage dans le dialogue établi entre la tumeur et son microenvironnement. Nous y détaillons, d'une part, les effets de la masse tumorale et son implication dans la modulation de la plasticité endothéliale et, d'autre part, la contribution de la niche vasculaire à la tumorigenèse. < précurseurs des cellules endothéliales dérivés du mésoderme et leur association en un réseau vasculaire primitif. Ce processus est principalement observé au cours des étapes précoces du dévelop-pement embryonnaire, puis lors de la vascularisation des ébauches d'organes. Par la suite, le réseau vasculaire se développe à partir de vaisseaux sanguins préexistants, principalement par prolifération et bourgeonnement des cellules endothéliales, ce qu'on appelle l'angiogenèse. Le système vasculaire forme un important réseau impliqué dans la distribution d'oxygène et de nutriments aux organes. Les cellules endothéliales qui tapissent la face interne des vaisseaux sont organisées en une fine monocouche et sont étroitement reliées entre elles par des jonctions cellulaires spécialisées. C'est cette barrière endothéliale, en interaction avec les péricytes et une matrice basale différenciée, qui définit les propriétés de la paroi vasculaire ( Figure 1A L'angiogenèse tumoraleOutre leur fonction d'échange, les vaisseaux sanguins contribuent activement aux processus de régénération et de croissance des tissus. Ces fonctions physiologiques peuvent être détournées en conditions pathologiques, notamment dans les cancers. En effet, la croissance de tumeurs solides au-delà de 1 à 2 mm 3 est dépendante du dévelop-pement d'un réseau vasculaire qui leur est consacré. L'angiogenèse

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Differential Proteomic Analysis of Human Glioblastoma and Neural Stem Cells Reveals HDGF as a Novel Angiogenic Secreted Factor

Cited by 71 publications

References 81 publications

How stem cells speak with host immune cells in inflammatory brain diseases

How stem cells speak with host immune cells in inflammatory brain diseases

Hepatoma-derived growth factor: from the bovine uterus to the in vitro embryo culture

Vaisseaux sanguins et tumeurs ou l’art du dialogue

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