Differential proteomics of Aedes albopictus salivary gland, midgut and C6/36 cell induced by dengue virus infection

Zhang, Meichun; Zheng, Xiaoying; Yu, Weiwu; Ming, Gan; He, Ai; Li, Zhuoya; Zhang, Dongjing; Wu, Xiansheng; Zhan, Xi

doi:10.1016/j.virol.2013.06.001

Cited by 26 publications

(21 citation statements)

References 63 publications

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“…Consistent with enzymes that are linked to protection, energy production, and protein metabolism, proteins that participate in signaling and transport (14-3-3 and WD40) [31,32] were also abundant in the NE. Interestingly, in the A. aegypti larvae midguts infected with CHIKV, WD-repeat proteins were highly expressed [5], while expression of 14-3-3 proteins was low in the midgut of mosquitoes infected with DENV-2 [30].…”

Section: Resultsmentioning

confidence: 99%

“…Four other proteins related to protein metabolism were abundant in the NE and BF: glutamate dehydrogenase, aspartate aminotransferase, PDI and calreticulin [20,[27][28][29]. Calreticulin and PDI are highly expressed during DENV-2 and CHIKV infection of the midgut, respectively, may be involved in the replication, translation, and/or viral encapsidation [5,30]. Three proteins involved in cell signaling -14-3-3 protein (NE72), 14-3-3 protein epsilon (YWHAE; NE71), and WD40-repeat (NE38)were identified, as well as proteins involved in transport: RabGEF (NE48), proteins that form voltage dependent ion channels (VDAC; NE41) and V-ATPase (BF18).…”

Section: Resultsmentioning

confidence: 99%

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Proteomic analysis of Aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets

Fernandes

Magalhães-Júnior

Baracat‐Pereira

et al. 2016

Parasitology International

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In this work we analyzed protein expression in the Aedes aegypti midgut during the larval (fourth instar, L4), pupal, and adult stages [including newly emerged (NE), sugar-fed (SF) and blood-fed (BF) females]. Two-dimensional electrophoresis showed 13 spots in the midgut of larvae, 95 in the midgut of pupae, 90 in the midgut of NE, and 76 in the midgut of SF or BF females. In the larval midguts, high serpin expression was noted, while in the pupae, protein abundance was lower than in the NE, SF, and BF females. The spots related to proteins linked to energy production, protein metabolism, signaling, and transport were highly expressed in the NE stage, while spots related proteins involved in translation were abundant in SF and BF females. The differential abundance of proteins in the midgut of A. aegypti at different developmental stages supports the necessity for midgut development during immature stage followed by the necessity of proteins related to digestion in adults.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of Aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets

Fernandes

Magalhães-Júnior

Baracat‐Pereira

et al. 2016

Parasitology International

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“…This effect was more significant in the depolymerization of microtubules that in actin microfilaments. It has been reported that viral infections alter the global host proteomic in response to these, including cytoskeletal proteins [87][88][89]. However, although a role for the involvement of these proteins in viral infection is hypothesized, a route through which this occurs has yet to be identified.…”

Section: Discussionmentioning

confidence: 99%

Cell Biology of Virus Infection. The Role of Cytoskeletal Dynamics Integrity in the Effectiveness of Dengue Virus Infection

Orozco-García¹,

Trujillo-Correa²,

CarlosGallego-Gómez³

2016

Cell Biology - New Insights

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The cell biology of viral infections is the focus of this research, in which the role of the cytoskeleton in dengue virus (DENV) replication in cell cultures was evaluated by means of Nocodazole and Cytochalasin D treatments before and after of DENV infection. The potential contribution of cytoskeleton elements with/without the treatment of depolymerizing agents was evidenced and quantified by the subcellular distribution of viral proteins, virions produced, and viral protein quantification. The cytoskeleton is involved in DENV replication because treatments with actin microfilaments and microtubule depolymerizing agents in non-cytotoxic concentrations, affected DENV2 replication in Vero cells and decreased both the viral protein expression and infectious virion production, when compared with non-treated cells. The actin and microtubules are partly involved in DENV2 replication, since the treatment does not completely blocked viral replication, suggesting that these components are necessary but not sufficient alone for DENV2 replication in Vero cells. The structural and functional role of actin and the microtubules in replication are postulated here, opening new perspectives for understanding the architecture of the replicative complex and viral morphogenesis processes, due to the role of the cytoskeleton in the organization, recruitment, and function of the cellular elements necessary for the assembly of viral factories.

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“…aegypti and Ae. albopictus [25,27,28], analysis of the actual saliva reveals reduced amounts of adenosine deaminase, aegyptin, and serpin [24]. Infection of Ae.…”

Section: Effects Of Environmental Factors and Pathogens In The Expresmentioning

confidence: 99%

Impact of insect salivary proteins in blood feeding, host immunity, disease, and in the development of biomarkers for vector exposure

Coutinho-Abreu

Guimarães-Costa

Valenzuela

2015

Current Opinion in Insect Science

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Functional genomic approaches based on expression of recombinant proteins linked to biochemical and disease model approaches resulted in the discovery of novel biological activities and the role some of these proteins play in disease transmission. Importantly, the expression of salivary proteins was recently shown to be affected by environmental factors and by the presence of the pathogen in the salivary gland. A practical application resulting from insect saliva research is the use of insect antigenic salivary protein as biomarkers of vector exposure in humans and animal reservoirs, an approach that is yielding interesting results in the field.

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Differential proteomics of Aedes albopictus salivary gland, midgut and C6/36 cell induced by dengue virus infection

Cited by 26 publications

References 63 publications

Proteomic analysis of Aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets

Proteomic analysis of Aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets

Cell Biology of Virus Infection. The Role of Cytoskeletal Dynamics Integrity in the Effectiveness of Dengue Virus Infection

Impact of insect salivary proteins in blood feeding, host immunity, disease, and in the development of biomarkers for vector exposure

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