Differential quantification of SCCA1 and SCCA2 cancer antigens using a hydrogel biochip

Tikhonov, Aleksei; Tsybulskaya, M. V.; Butvilovskaya, V. I.; Savvateeva, Elena; Belousov, Pavel V.; Kuprash, Dmitry V.; Солопова, О. Н.; Черниченко, М. А.; Filushin, M.; Rubina, A. Yu.

doi:10.1039/c6ay02216b

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…As an alternative to the binding of molecules through Coulombic interactions, proteins can also be entrapped in hydrogel matrixes for microarray analysis. These gel-entrapped proteins remain structurally intact and maintain their biological activity due to the relatively inert, aqueous environment. − Sol–gel-based immobilization matrixes have also been reported for protein analysis . Gel-immobilization of functionalized nanoparticles has been reported for metal-enhanced fluorescence …”

Section: Immobilization Chemistries For Microarray Surfacesmentioning

confidence: 99%

The Surface Science of Microarray Generation–A Critical Inventory

Brittain

Brandsetter

Prucker

et al. 2019

ACS Appl. Mater. Interfaces

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Microarrays are powerful tools in biomedical research and have become indispensable for high-throughput multiplex analysis, especially for DNA and protein analysis. The basis for all microarray processing and fabrication is surface modification of a chip substrate and many different strategies to couple probe molecules to such substrates have been developed. We present here a critical assessment of typical biochip generation processes from a surface science point of view. While great progress has been made from a molecular biology point of view on the development of qualitative assays and impressive results have been obtained on the detection of rather low concentrations of DNA or proteins, quantitative chip-based assays are still comparably rare. We argue that lack of stable and reliable deposition chemistries has led in many cases to suboptimal quantitative reproducibility, impeded further progress in microarray development and prevented a more significant penetration of microarray technology into the diagnostic market. We suggest that surface-attached hydrogel networks might be a promising strategy to achieve highly sensitive and quantitatively reproducible microarrays.

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Section: Immobilization Chemistries For Microarray Surfacesmentioning

confidence: 99%

The Surface Science of Microarray Generation–A Critical Inventory

Brittain

Brandsetter

Prucker

et al. 2019

ACS Appl. Mater. Interfaces

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“…6 Squamous cell carcinoma antigen (SCCA) is isolated from squamous cell carcinoma of the uterine cervix and is characterized as a glycoprotein with a molecular mass of 48 kDa. 7,8 SCCA is one of the auxiliary indicators for cervical cancer diagnosis, condition detection and prognosis judgment. 9 Cancer antigen 125 (CA125) is a common marker in the detection of female reproductive system tumors, where the level of CA125 is elevated signicantly in the serum of patients with cervical cancer.…”

Section: Introductionmentioning

confidence: 99%

The simultaneous detection of the squamous cell carcinoma antigen and cancer antigen 125 in the cervical cancer serum using nano-Ag polydopamine nanospheres in an SERS-based lateral flow immunoassay

Liu

Ran

et al. 2020

RSC Adv.

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“…Следовательно, необходимы полимерные или металлические метки при условии достаточной адгезии используемого материала к используемой поверхности. Формирование полимерных меток связано с процессами полимеризации (в частности, многоступенчатыми) и/или травления [14][15]. Более того, образующиеся в результате олигомеры и остаточные количества непрореагировавших мономерных компонентов могут вызвать загрязнения поверхности АСМ-чипа при его дальнейшем использовании для фишинга белка.…”

Section: Introductionunclassified