Differential regional and cellular distribution of TFF3 peptide in the human brain

Bernstein, Hans‐Gert; Dobrowolny, Henrik; Trübner, Kurt; Steiner, Johann; Bogerts, Bernhard; Hoffmann, Werner

doi:10.1007/s00726-015-1938-9

Cited by 18 publications

(12 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TFF3 peptides are located to mucous epithelia as well as nervous tissue and are involved in apoptosis, cell migration and immune responses (Belovari et al, ). In disease, TFF3 has been associated with cell growth, metastasis and angiogenesis in cancer (Babyatsky et al, ; Kjellev, ) as well as with neurodegenerative disorders such as Alzheimer's disease (Bernstein et al, ). This protein has never been associated to retinal gliosis and its precise role in inflammatory processes affecting the retina is not known.…”

Section: Discussionmentioning

confidence: 99%

Müller glia as an important source of cytokines and inflammatory factors present in the gliotic retina during proliferative vitreoretinopathy

Eastlake¹,

Banerjee²,

Angbohang³

et al. 2015

Glia

112

View full text Add to dashboard Cite

Retinal gliosis is characterized by biochemical and physiological changes that often lead to Müller glia proliferation and hypertrophy and is a feature of many neuro‐degenerative and inflammatory diseases such as proliferative vitreoretinopathy (PVR). Although Müller glia are known to release inflammatory factors and cytokines, it is not clear whether cytokine production by these cells mirrors the pattern of factors present in the gliotic retina. Lysates from normal cadaveric retina and gliotic retinal specimens from patients undergoing retinectomy for treatment of PVR, the Müller cell line MIO‐M1 and four human Müller glial cell preparations isolated from normal retina were examined for their expression of cytokines and inflammatory factors using semi‐quantitative dot blot antibody arrays and quantitative arrays. Comparative analysis of the expression of inflammatory factors showed that in comparison with normal retina, gliotic retina exhibited greater than twofold increase in 24/102 factors examined by semiquantitative arrays, and a significant increase in 19 out of 27 factors assessed by quantitative methods (P < 0.05 to P < 0.001). It was observed that with the exception of some chemotactic factors, the majority of cytokines and inflammatory factors were produced by Müller glia in vitro and included G‐CSF, MCP‐1, PDGF‐bb, RANTES, VEGF, and TGFβ2. These results showed that a large number of inflammatory factors expressed by Müller glia in vitro are upregulated in the gliotic retina, suggesting that targeting the production of inflammatory factors by Müller glia may constitute a valid approach to prevent neural damage during retinal gliosis and this merits further investigations. GLIA 2016;64:495–506

show abstract

Section: Discussionmentioning

confidence: 99%

Müller glia as an important source of cytokines and inflammatory factors present in the gliotic retina during proliferative vitreoretinopathy

Eastlake¹,

Banerjee²,

Angbohang³

et al. 2015

Glia

112

View full text Add to dashboard Cite

show abstract

“…TFF proteins are secreted-type proteins mostly existing in the mucous of the GI tract as well as in serum and urine [63] with minute amounts within the brain [64,65].…”

Section: Distribution and Expressionmentioning

confidence: 99%

Trefoil factors: Gastrointestinal-specific proteins associated with gastric cancer

Xiao

Ling

Lan

et al. 2015

Clinica Chimica Acta

View full text Add to dashboard Cite

“…Besides their expression in mucous epithelia, TFF peptides are synthesized in the central nervous system and ocular tissues of rodents and humans [ 8 – 10 ]. Our group was the first to investigate retinal expression of TFF peptides.…”

Section: Introductionmentioning

confidence: 99%

Forced Trefoil Factor Family Peptide 3 (TFF3) Expression Reduces Growth, Viability, and Tumorigenicity of Human Retinoblastoma Cell Lines

et al. 2016

View full text Add to dashboard Cite

Trefoil factor family (TFF) peptides have been shown to effect cell proliferation, apoptosis, migration and invasion of normal cells and various cancer cell lines. In the literature TFF peptides are controversially discussed as tumor suppressors and potential tumor progression factors. In the study presented, we investigated the effect of TFF3 overexpression on growth, viability, migration and tumorigenicity of the human retinoblastoma cell lines Y-79, WERI-Rb1, RBL-13 and RBL-15. As revealed by WST-1 and TUNEL assays as well as DAPI and BrdU cell counts, recombinant human TFF3 significantly lowers retinoblastoma cell viability and increases apoptosis levels. Transient TFF3 overexpression likewise significantly increases RB cell apoptosis. Stable, lentiviral TFF3 overexpression lowers retinoblastoma cell viability, proliferation and growth and significantly increases cell death in retinoblastoma cells. Blockage experiments using a broad-spectrum caspase inhibitor and capase-3 immunocytochemistry revealed the involvement of caspases in general and of caspase-3 in particular in TFF3 induced apoptosis in retinoblastoma cell lines. Soft agarose and in ovo chicken chorioallantoic membrane (CAM) assays revealed that TFF3 overexpression influences anchorage independent growth and significantly decreases the size of tumors forming from retinoblastoma cells. Our study demonstrates that forced TFF3 expression exerts a significant pro-apoptotic, anti-proliferative, and tumor suppressive effect in retinoblastoma cells, setting a starting point for new additive chemotherapeutic approaches in the treatment of retinoblastoma.

show abstract

Differential regional and cellular distribution of TFF3 peptide in the human brain

Cited by 18 publications

References 39 publications

Müller glia as an important source of cytokines and inflammatory factors present in the gliotic retina during proliferative vitreoretinopathy

Müller glia as an important source of cytokines and inflammatory factors present in the gliotic retina during proliferative vitreoretinopathy

Trefoil factors: Gastrointestinal-specific proteins associated with gastric cancer

Forced Trefoil Factor Family Peptide 3 (TFF3) Expression Reduces Growth, Viability, and Tumorigenicity of Human Retinoblastoma Cell Lines

Contact Info

Product

Resources

About