Differential requirement for Bub1 and Bub3 in regulation of meiotic versus mitotic chromosome segregation

Cairo, Gisela; MacKenzie, Anne; Lacefield, Soni

doi:10.1083/jcb.201909136

Cited by 19 publications

(54 citation statements)

References 81 publications

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“…There is yet no evidence this mechanism is important in budding yeast. However, consistent with this model is the recent demonstration that BUB1 and BUB3 mutants, like MPS1 mutants, both exhibit much higher levels of meiotic than mitotic segregation defects and mis-segregate homologous chromosomes to the older SPB in meiosis I, though not at the high levels seen in MPS1 mutants (Cairo et al, 2020).…”

Section: Discussionmentioning

confidence: 54%

Mps1 promotes poleward chromosome movements in meiotic pro-metaphase

Meyer

Tipton

Gorbsky

et al. 2020

Preprint

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ABSTRACTIn prophase of meiosis I, homologous partner chromosomes pair and become connected by crossovers. Chiasmata, the connections formed between the partners enable the chromosome pair, called a bivalent to attach as a single unit to the spindle. When the meiosis I spindle forms, most bivalents are associated with a single spindle pole and go through a series of oscillations on the spindle attaching and detaching from microtubules until the partners of the bivalent are bi-oriented, that is, attached to microtubules from opposite sides of the spindle, and prepared to be segregated at anaphase I. The conserved, kinetochore-associated kinase, Mps1, is essential for the bivalents to be pulled by microtubules across the spindle. Here we explore the role of Mps1 in this process. In MPS1 mutants, kinetochores can attach to microtubules and, when they are pulled by microtubule de-polymerization, can move at normal speeds across the spindle. In MPS1 mutants, kinetochores often become locked-in-place on the pro-metaphase spindle and pro-metaphase kinetochore microtubules reduced turnover. Together the results suggest that Mps1 is necessary to promote depolymerization of kinetochore-microtubules during the bi-orientation process.

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Section: Discussionmentioning

confidence: 54%

Mps1 promotes poleward chromosome movements in meiotic pro-metaphase

Meyer

Tipton

Gorbsky

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…There is as yet no evidence that this mechanism is important in budding yeast. However, consistent with this model is the recent demonstration that BUB1 and BUB3 mutants, like MPS1 mutants, both exhibit much higher levels of meiotic than mitotic segregation defects and missegregate homologous chromosomes to the older SPB in meiosis I, though not at the high levels seen in MPS1 mutants ( Cairo et al. , 2020 ).…”

Section: Discussionmentioning

confidence: 54%

Mps1 promotes poleward chromosome movements in meiotic prometaphase

Meyer

Tipton

LaVictoire

et al. 2021

MBoC

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In prophase of meiosis I, homologous chromosomes pair and become connected by crossovers. Chiasmata, the connections formed by crossovers, enable the chromosome pair, called a bivalent, to attach as a single unit to the spindle. When the meiotic spindle forms in prometaphase, most bivalents are associated with one spindle pole and then go through a series of oscillations on the spindle, attaching to and detaching from microtubules until the partners of the bivalent become bioriented - attached to microtubules from opposite sides of the spindle. The conserved kinase, Mps1, is essential for the bivalents to be pulled by microtubules across the spindle in prometaphase. Here we show that MPS1 is needed for efficient triggering of the migration of microtubule-attached kinetochores towards the poles and promotes microtubule depolymerization. Our data support the model Mps1 acts at the kinetochore to co-ordinate the successful attachment of a microtubule and the triggering of microtubule depolymerization to then move the chromosome.

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“…Depletion of Bub3 also results in shorter metaphase I and metaphase II due to premature localization of protein phosphatase 1 (PP1) to kinetochores, which antagonizes Ipl1mediated phosphorylation. they propose a new role for the Bub1-Bub3 pathway in maintaining the balance between kinetochore localization of Ipl1 and PP1, a balance that is essential for accurate meiotic chromosome segregation and timely anaphase onset 12 .…”

Section: Discussionmentioning

confidence: 99%

Aneuploid Abortion Positively Correlate With MAD1 Overexpression and mir125b Depression

Zhao¹,

Li²,

Li³

et al. 2020

Preprint

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Background: Aneuploid is the most frequent cause of early embryo abortion, and any defect in chromosome segregation would fail to satisfy spindle assembly checkpoint (SAC) during mitosis, which could lead to the halted metaphase and aneuploid occurrence. Mitotic checkpoint complex(MCC), a complex compound of MAD1、MAD2、Cdc20、BUBR1 and BUB3, plays an important role in SAC activation. Studies have confirmed that the overexpression of MAD2 and BUBR1 can facilitate the correct chromosome segregation and embryo stability. Research identifications also proved that miR-125b negatively regulated MAD1 expression by binding to its 3’UTR. However, the expression of mir125b, MAD1 and BUB3 genes in aneuploidy embryos of spontaneous abortion has not been reported.Methods: In this study, embryonic villi from miscarriage pregnant women were collected and divided into two groups (aneuploidy and euploidy) by HLPA and FISH analysis. The RNA levels of mir125b, MAD1 and BUB3 were detected through QRT-PCR, while Western blot was further used to analyze the protein levels of MAD1 and BUB3.Results: SPSS 17.0 statistical analysis(P<0.05) showed that mir125b and BUB3 were significantly down-regulated in aneuploidy group compared to the control group, MAD1 was significantly up-regulated in RNA level; Additionally, MAD1 protein level was also significantly higher while BUB3 was mildly increased in aneuploidy abortion villus. Correlation analysis revealed that the expression of MAD1 was negatively correlated with Mir125b.Conclusion: these results suggested that aneuploid abortion was positively correlated with MAD1 overexpression which might be caused by insufficient mir125b.

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Differential requirement for Bub1 and Bub3 in regulation of meiotic versus mitotic chromosome segregation

Cited by 19 publications

References 81 publications

Mps1 promotes poleward chromosome movements in meiotic pro-metaphase

Mps1 promotes poleward chromosome movements in meiotic pro-metaphase

Mps1 promotes poleward chromosome movements in meiotic prometaphase

Aneuploid Abortion Positively Correlate With MAD1 Overexpression and mir125b Depression

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