Differential response of MCF7, MDA-MB-231, and MCF 10A cells to hyperthermia, silver nanoparticles and silver nanoparticle-induced photothermal therapy

Thompson, Edreca A; Graham, Elizabeth G.; MacNeill, Christopher M.; Young, Michelle; Donati, George L.; Wailes, Elizabeth M.; Jones, Bradley T.; Levi‐Polyachenko, Nicole

doi:10.3109/02656736.2014.936051

Cited by 64 publications

(48 citation statements)

References 21 publications

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“…5, 25, 50, and 75 nm in diameter spherical AgNPs stabilized with polyvinylpyrrolidone (PVP; 89.3, 85, 76.5, 74% by mass, respectively), PVP and silica shelled triangular silver nanoplates, and PVP‐stabilized 15 nm gold nanoparticles (AuNPs; 91.6% PVP by mass) were purchased as dried nanopowders from nanoComposix, Inc. Free (ionic) silver represented less than 1% of the total nanoparticle silver content according the manufacturer's specifications. Chitosan‐coated AgNPs were synthesized according to previously published methods . Nanoparticles were dispersed at a concentration of 20 mg/mL (total nanoparticle weight) in phosphate buffered saline (PBS) (Invitrogen) by bath sonication, and then were diluted in cell culture medium to the final concentration listed in the figures prior to addition to the wells containing cells.…”

Section: Methodsmentioning

confidence: 99%

“…Chitosan-coated AgNPs were synthesized according to previously published methods. 43 Nanoparticles were dispersed at a concentration of 20 mg/mL (total nanoparticle weight) in phosphate buffered saline (PBS) (Invitrogen) by bath sonication, and then were diluted in cell culture medium to the final concentration listed in the figures prior to addition to the wells containing cells.…”

Section: Introductionmentioning

confidence: 99%

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Silver nanoparticles selectively treat triple‐negative breast cancer cells without affecting non‐malignant breast epithelial cells in vitro and in vivo

et al. 2019

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Silver nanoparticles (AgNPs) show promise for treatment of aggressive cancers including triple‐negative breast cancer (TNBC) in preclinical cancer models. For clinical development of AgNP‐based therapeutics, it will be necessary to clearly define the specific physicochemical features of the nanoparticles that will be used, and to tie these properties to biological outcomes. To fill this knowledge gap, we performed thorough structure/function, mechanistic, safety, and efficacy studies to assess the potential for AgNPs to treat TNBC. We establish that AgNPs, regardless of size, shape, or stabilizing agent, are highly cytotoxic to TNBC cells at doses that are not cytotoxic to non‐malignant breast epithelial cells. In contrast, TNBC cells and non‐malignant breast epithelial cells are similarly sensitive to exposure to silver cation (Ag+), indicating that the nanoparticle formulation is essential for the TNBC‐specific cytotoxicity. Mechanistically, AgNPs are internalized by both TNBC and non‐malignant breast cells, but are rapidly degraded only in TNBC cells. Exposure to AgNPs depletes cellular antioxidants and causes endoplasmic reticulum stress in TNBC cells without causing similar damage in non‐malignant breast epithelial cells. AgNPs also cause extensive DNA damage in 3D TNBC tumor nodules in vitro, but do not disrupt the normal architecture of breast acini in 3D cell culture, nor cause DNA damage or induce apoptosis in these structures. Lastly, we show that systemically administered AgNPs are effective at non‐toxic doses for reducing the growth of TNBC tumor xenografts in mice. This work provides a rationale for development of AgNPs as a safe and specific TNBC treatment.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles selectively treat triple‐negative breast cancer cells without affecting non‐malignant breast epithelial cells in vitro and in vivo

et al. 2019

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“…Silver nanoparticles are inherently highly effective against microorganisms by inhibiting bacterial growth on medical devices and burns, especially dependent upon their surface area [119][120][121]. They can be made more anisotropic to facilitate the shift of their plasmon resonance, and hence optical absorption, into the NIR, so that they can be used as photothermal agents [122]. Many NP designed to couple photothermal heating with delivery utilise classic antibiotics.…”

Section: Silver Nanoparticlesmentioning

confidence: 99%

Progress on utilizing hyperthermia for mitigating bacterial infections

Ibelli

Templeton

Levi‐Polyachenko

2018

International Journal of Hyperthermia

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Recovery from systemic or local bacterial infections can be lengthy and costly, with the clinical challenges being further complicated when bacteria acquire resistance to current antibiotics. Hyperthermia offers new mechanisms for removing bacteria via ablation, or sensitising them to chemical agents. The first part of this review provides a background on the bacterial biofilms, their response to hyperthermia, and acquired resistance to antibiotics, followed by the clinical challenges they present in managing infections associated with soft tissues and biomedical implants. The second part of the review discusses the thermal modalities used to combat infections, including radiofrequency, ultrasound, highintensity focussed ultrasound, microwave thermotherapy, and photothermal and magnetic nanoparticles (NP). The overall aim of this review is to demonstrate the tremendous potential of hyperthermia for mitigating bacterial infections and foster new research ventures to help remedy these challenging occurrences.

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“…Despite the advantages of the use of AuNPs, some studies report the use of AgNPs in photothermal therapy against different types of cancer, including ovarian cancer [64]. AgNPs have photothermal and cytotoxic properties, which can be used to generate a synergistic effect [61,65,66], which leads to the death of cancer cells. The type of nanostructures that have frequently been used in the field of biomedicine includes nanospheres, nanowires, nanorods, nanoplates and nanocubes [2,22,25,50,56,74].…”

Section: Introductionmentioning

confidence: 99%

AOT direct and reverse micelles as a reaction media for anisotropic silver nanoparticles functionalized with folic acid as a photothermal agent on HeLa cells

et al. 2019

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Herein, we report the synthesis of silver nanoparticles (AgNPs) using direct micelles and reverse micelles of sodium bis (2-ethylhexyl) sulfosuccinate (AOT) as reaction media. We evaluated the effect of these self-organized aggregates in the nucleation and growth mechanism of AgNPs. The results show the effect of water when it is a confined medium, where their properties are different from those of net water. These factors strongly influence the size, polydispersity and shape of nanoparticles. Moreover, the presence of ions from the reducing agents and the surfactant, it is a key factor in the anisotropy of the AgNPs obtained. For our knowledge, this is the first report in which the effect of the reaction media is evaluated by using direct and reverse micelles to synthesize silver nanoparticles. Additionally, AgNPs synthesized through the two systems were functionalized with folic acid and evaluated their cytotoxicity and photothermal properties. The results show that AgNPs synthesized through the two systems are potential photothermal agents, reaching to reduce up to 47% of the viability of the HeLa cells, using a laser of 808 nm.

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Differential response of MCF7, MDA-MB-231, and MCF 10A cells to hyperthermia, silver nanoparticles and silver nanoparticle-induced photothermal therapy

Abstract: Ag NP are effective photothermal agents. A secondary benefit is the differential response of breast cancer cells to Ag NP-induced hyperthermia, due to increased intracellular silver content, compared to non-tumorigenic breast epithelial cells.

Cited by 64 publications

References 21 publications

Silver nanoparticles selectively treat triple‐negative breast cancer cells without affecting non‐malignant breast epithelial cells in vitro and in vivo

Silver nanoparticles selectively treat triple‐negative breast cancer cells without affecting non‐malignant breast epithelial cells in vitro and in vivo

Progress on utilizing hyperthermia for mitigating bacterial infections

AOT direct and reverse micelles as a reaction media for anisotropic silver nanoparticles functionalized with folic acid as a photothermal agent on HeLa cells

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