Differential roles of STIM1, STIM2 and Orai1 in the control of cell proliferation and SOCE amplitude in HEK293 cells

Integrated Analysis Reveals Critical Genomic Regions in Prostate Tumor Microenvironment Associated with Clinicopathologic Phenotypes

“…STIM1 was originally proposed as a candidate tumor suppressor and regulates intracellular calcium (40,41). STIM2 is also a transmembrane protein that, in limited studies, has been shown to mediate cellular proliferation (42). In view of our observations, further studies on the role of STIM2 in different cancers, especially in different compartments, are required.…”

Store-Operated Ca2+ Entry

“…reported that STIM2 might mediate SOCE via two store-dependent and store-independent modes in HEK293 cells [ 124 ] . Other studies observed a minor decrease of SOCE in the absence of STIM2 function in murine embryonic fi broblasts (MEF), B and T cells, rat pulmonary arterial smooth muscle cells and some cell lines such as breast cancer, HeLa or HEK293 cells [ 81,106,107,109,125,127,133 ] , while recent reports suggested a prominent function of STIM2 in SOCE in murine neurons, HEK293 and dendritic cells [ 103,134,135 ] . Thus, STIM2 showed an involvement in SOCE in different degrees depending of the cell type, which might be explained by partial compensation of STIM1, probably depending of the endogenous level of both STIMs in these cells.…”

“…Finally, recent reports suggested a role of STIM2 in HEK293 cell proliferation in vitro [ 135 ] , in vivo secretion of cytokines in murine T and B cells [ 106,107,109 ] , neuronal cell death during hypoxic conditions [ 103 ] and development of autoimmune responses [ 106,109 ] , thus indicating the importance of STIM2 function in basic cellular functions and in development of pathologic conditions.…”

Immunodéficiences et pathologies associées aux mutations dans STIM/ORAI

“…Cependant, les récentes données portant sur le rôle de STIM1 dans la prolifération cellulaire conduisent à des conclusions opposées. Un ARN interférent (siARN) dirigé contre STIM1 soit est sans effet, soit inhibe complète-ment la prolifération en fonction du type cellulaire [30,31]. En revanche, il y a un consensus à l'heure actuelle pour admettre qu'il existe une inhibition de la proliféra-tion cellulaire en présence des siARN contre STIM2 [31], ORAI1 [31] et ORAI3 [32].…”

“…Un ARN interférent (siARN) dirigé contre STIM1 soit est sans effet, soit inhibe complète-ment la prolifération en fonction du type cellulaire [30,31]. En revanche, il y a un consensus à l'heure actuelle pour admettre qu'il existe une inhibition de la proliféra-tion cellulaire en présence des siARN contre STIM2 [31], ORAI1 [31] et ORAI3 [32]. Certains de ces effets des STIM et ORAI passent probablement par une activation des complexes cyclines-cdk (cyclin-dependent kinase) contrôlant la progression dans le cycle cellulaire [31][32][33].…”