Differential Sperm Proteomics Reveals the Significance of Fatty Acid Synthase and Clusterin in Idiopathic Recurrent Pregnancy Loss

Thapliyal, Ayushi; Tomar, Amit; Chandra, Kumari Binita; Naglot, Sarla; Dhiman, Soniya; Singh, Neeta; Sharma, Jai Bhagwan; Yadav, Savita

doi:10.1007/s43032-023-01288-8

Cited by 4 publications

(5 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On searching miRWalk, it was found that GPX4 is a gene target of hsa-miR-1290 (score = 0.846). Interestingly, reduced GPX4 expression in spermatozoa samples from male partners of iRPL patients was also observed in one of our previous studies [ 16 ]. This reduction likely impairs the spermatozoa’s ability to defend against the detrimental effects of lipid peroxidation, significantly impacting sperm quality.…”

Section: Discussionsupporting

confidence: 82%

“…In recent years, paternal factors such as DNA alterations, sperm proteomic changes, and reactive oxygen species (ROS) levels leading to poor sperm quality have been extensively studied [ 15 , 28 , 29 , 30 , 31 ]. Furthermore, differential sperm proteomics studies have revealed the altered expression of proteins associated with various biological processes such as oxidative stress, stress response, and protein folding/refolding, in iRPL [ 14 , 15 , 16 , 32 ]. However, the role of male contributory factors and the associated biological processes leading to iRPL remain inadequately understood, underscoring the necessity for integrative molecular studies to achieve a comprehensive understanding of iRPL.…”

Section: Discussionmentioning

confidence: 99%

“…This finding aligns with proteomics studies that indicate increased oxidative stress in iRPL spermatozoa, which may contribute to reduced sperm quality. This reduction is attributed to the downregulated expression of proteins associated with the oxidative stress response and DNA damage repair [ 14 , 15 , 16 ]. Heat shock proteins (HSPs) are abundantly present in spermatozoa and are crucial for various sperm functions, including sperm vitality, motility, and capacitation [ 58 , 59 , 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, the significance of these factors in RPL remains inconclusive due to contradictory studies [ 12 , 13 ]. Recent proteomics studies on male partners of iRPL patients have unveiled differentially expressed sperm proteins associated with oxidative stress and sperm quality [ 14 , 15 , 16 ]. These findings suggest a significant molecular association between male fertility and pregnancy outcomes.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Exploring Differentially Expressed Sperm miRNAs in Idiopathic Recurrent Pregnancy Loss and Their Association with Early Embryonic Development

Thapliyal,

Tomar,

Naglot

et al. 2024

ncRNA

Self Cite

View full text Add to dashboard Cite

The high incidence of idiopathic recurrent pregnancy loss (iRPL) may stem from the limited research on male contributory factors. Many studies suggest that sperm DNA fragmentation and oxidative stress contribute to iRPL, but their roles are still debated. MicroRNAs (miRNAs) are short non-coding RNAs that regulate various biological processes by modulating gene expression. While differential expression of specific miRNAs has been observed in women suffering from recurrent miscarriages, paternal miRNAs remain unexplored. We hypothesize that analyzing sperm miRNAs can provide crucial insights into the pathophysiology of iRPL. Therefore, this study aims to identify dysregulated miRNAs in the spermatozoa of male partners of iRPL patients. Total mRNA was extracted from sperm samples of iRPL and control groups, followed by miRNA library preparation and high-output miRNA sequencing. Subsequently, raw sequence reads were processed for differential expression analysis, target prediction, and bioinformatics analysis. Twelve differentially expressed miRNAs were identified in the iRPL group, with eight miRNAs upregulated (hsa-miR-4454, hsa-miR-142-3p, hsa-miR-145-5p, hsa-miR-1290, hsa-miR-1246, hsa-miR-7977, hsa-miR-449c-5p, and hsa-miR-92b-3p) and four downregulated (hsa-miR-29c-3p, hsa-miR-30b-5p, hsa-miR-519a-2-5p, and hsa-miR-520b-5p). Functional enrichment analysis revealed that gene targets of the upregulated miRNAs are involved in various biological processes closely associated with sperm quality and embryonic development.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exploring Differentially Expressed Sperm miRNAs in Idiopathic Recurrent Pregnancy Loss and Their Association with Early Embryonic Development

Thapliyal,

Tomar,

Naglot

et al. 2024

ncRNA

Self Cite

View full text Add to dashboard Cite

show abstract

“…FASN and CLU were identified as participants in oxidoreductase activity and metabolic pathways, being more abundant in individuals from the wild. Both FASN and CLU have been recognized as potential sperm markers for recurrent pregnancy loss [70][71][72]. Lipids constitute a major component of teleost fish spermatozoa [73].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Insights into Seminal Plasma and Spermatozoa Proteins of Small-Spotted Catsharks, Scyliorhinus canicula: Implications for Reproductive Conservation in Aquariums

Muñoz-Baquero,

Lorenzo-Rebenaque,

García-Domínguez

et al. 2024

Animals

View full text Add to dashboard Cite

In the ex situ conservation of chondrichthyan species, successful reproduction in aquaria is essential. However, these species often exhibit reduced reproductive success under human care. A key aspect is that conventional sperm analyses do not provide insights into the functional competence of sperm. However, proteomics analysis enables a better understanding of male physiology, gaining relevance as a powerful tool for discovering protein biomarkers related to fertility. The present work aims to build the first proteome database for shark semen and to investigate the proteomic profiles of seminal plasma and spermatozoa from small-spotted catsharks (Scyliorhinus canicula) related to the underlying adaptations to both natural and aquarium environments, thereby identifying the reproductive impact in aquarium specimens. A total of 305 seminal plasma and 535 spermatozoa proteins were identified. Among these, 89 proteins (29.2% of the seminal plasma set) were common to both spermatozoa and seminal plasma. In the seminal plasma, only adenosylhomocysteinase protein showed differential abundance (DAP) between wild and aquarium animals. With respect to the spermatozoa proteins, a total of 107 DAPs were found between groups. Gene Ontology enrichment analysis highlighted the primary functional roles of these DAPs involved in oxidoreductase activity. Additionally, KEGG analysis indicated that these DAPs were primarily associated with metabolic pathways and carbon metabolism. In conclusion, we have successfully generated an initial proteome database for S. canicula seminal plasma and spermatozoa. Furthermore, we have identified protein variations, predominantly within spermatozoa, between aquarium and wild populations of S. canicula. These findings provide a foundation for future biomarker discovery in shark reproduction studies. However, additional research is required to determine whether these protein variations correlate with reproductive declines in captive sharks.

show abstract

Quantitative Proteomics of COVID-19 Recovered Patients Identifies Long-Term Changes in Sperm Proteins Leading to Cellular Stress in Spermatozoa

Chopra,

Tomar,

Thapliyal

et al. 2024

Reprod. Sci.

View full text Add to dashboard Cite

Differential Sperm Proteomics Reveals the Significance of Fatty Acid Synthase and Clusterin in Idiopathic Recurrent Pregnancy Loss

Cited by 4 publications

References 55 publications

Exploring Differentially Expressed Sperm miRNAs in Idiopathic Recurrent Pregnancy Loss and Their Association with Early Embryonic Development

Exploring Differentially Expressed Sperm miRNAs in Idiopathic Recurrent Pregnancy Loss and Their Association with Early Embryonic Development

Proteomic Insights into Seminal Plasma and Spermatozoa Proteins of Small-Spotted Catsharks, Scyliorhinus canicula: Implications for Reproductive Conservation in Aquariums

Quantitative Proteomics of COVID-19 Recovered Patients Identifies Long-Term Changes in Sperm Proteins Leading to Cellular Stress in Spermatozoa

Contact Info

Product

Resources

About