Differential Spleen miRNA Expression Profile of Beagle Dogs Infected with Toxocara canis

Xu, Yue; Li, Haoyu; Cai, Lang; Xie, Shi-Chen; Zou, Yang; Zhu, Xing‐Quan; Zheng, Wen-Bin

doi:10.3390/ani12192638

Cited by 2 publications

(1 citation statement)

References 56 publications

(59 reference statements)

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“…In the 2 of 13 study of parasites, miRNAs interact with the host and the parasite to influence processes such as immune regulation and gene regulation in vivo [8]. Many studies have shown that parasitic infections cause alterations in the host miRNA expression profile [9]. A cryptosporidium infection causes changes in the miRNA expression profile in host epithelial cells [10].…”

Section: Introductionmentioning

confidence: 99%

Identification of miRNA Associated with Trichomonas gallinae Resistance in Pigeon (Columba livia)

Li,

Ni,

Zhang

et al. 2023

IJMS

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Trichomonas gallinae (T. gallinae) has a great influence on the pigeon industry. Pigeons display different resistance abilities to T. gallinae, so the study of the molecular mechanism of resistance is necessary in breeding disease resistant lines. MiRNA plays important roles in the immune response, but there are still no reports of miRNA regulating trichomonosis resistance. We used small RNA sequencing technology to characterize miRNA profiles in different groups. T. gallinae was nasally inoculated in one day old squabs, and according to the infection status, the groups were divided into control (C), susceptible (S) and tolerant (T) groups. We identified 2429 miRNAs in total, including 1162 known miRNAs and 1267 new miRNAs. In a comparison among the C, S and T groups, the target genes of differentially expressed miRNAs were analyzed via GO and KEGG annotation. The results showed that the target genes were enriched in immune-response-related pathways. This indicated that the differentially expressed miRNAs had a critical influence on T. gallinae infection. Novel_miR_741, which could inhibit the expression of PRKCQ, was down-regulated in the T group compared to the C group. It was proven that a decreased novel_miR_741 expression would increase the expression of PRKCQ and increase the immune response. This study brings new insights into understanding the mechanism of trichomonosis resistance.

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Section: Introductionmentioning

confidence: 99%

Identification of miRNA Associated with Trichomonas gallinae Resistance in Pigeon (Columba livia)

Li,

Ni,

Zhang

et al. 2023

IJMS

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Alterations of plasma circulating microRNAs in BALB/c mice with Toxocara canis visceral and cerebral larva migrans

Yang,

Chen,

Zheng

et al. 2024

Parasites Vectors

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Background Human toxocariasis is a neglected parasitic disease characterised by the syndromes visceral, cerebral, and ocular larva migrans. This disease is caused by the migrating larvae of Toxocara roundworms from dogs and cats, affecting 1.4 billion people globally. Via extracellular vesicles (EVs), microRNAs have been demonstrated to play roles in host–parasite interactions and proposed as circulating biomarkers for the diagnosis and follow-up of parasitic diseases. Methods Small RNA-seq was conducted to identify miRNAs in the infective larvae of T. canis and plasma EV-containing preparations of infected BALB/c mice. Differential expression analysis and target prediction were performed to indicate miRNAs involved in host–parasite interactions and miRNAs associated with visceral and/or cerebral larva migrans in the infected mice. Quantitative real-time polymerase chain reaction (PCR) was used to amplify circulating miRNAs from the infected mice. Results This study reports host and parasite miRNAs in the plasma of BALB/c mice with visceral and cerebral larva migrans and demonstrates the alterations of these miRNAs during the migration of larvae from the livers through the lungs and to the brains of infected mice. After filtering unspecific changes in an irrelevant control, T. canis-derived miRNAs and T. canis infection-induced differential miRNAs are predicted to modulate genes consistently involved in mitogen-activated protein kinase (MAPK) signalling and pathways regulating axon guidance and pluripotency of stem in the infected mice with visceral and cerebral larva migrans. For these plasma circulating miRNAs predicted to be involved in host-parasite crosstalk, two murine miRNAs (miR-26b-5p and miR-122-5p) are experimentally verified to be responsive to larva migrans and represent circulating biomarker candidates for visceral and cerebral toxocariasis in BALB/c mice. Conclusions Our findings provide novel insights into the crosstalk of T. canis and the mammalian host via plasma circulating miRNAs, and prime agents and indicators for visceral and cerebral larva migrans. A deep understanding of these aspects will underpin the diagnosis and control of toxocariasis in humans and animals. Graphical Abstract

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Differential Spleen miRNA Expression Profile of Beagle Dogs Infected with Toxocara canis

Cited by 2 publications

References 56 publications

Identification of miRNA Associated with Trichomonas gallinae Resistance in Pigeon (Columba livia)

Identification of miRNA Associated with Trichomonas gallinae Resistance in Pigeon (Columba livia)

Alterations of plasma circulating microRNAs in BALB/c mice with Toxocara canis visceral and cerebral larva migrans

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