Differential Staining of Collagens Type I, II and III by Sirius Red and Polarization Microscopy

Junqueira, L. C.; Cossermelli, W; Brentani, Ricardo R.

doi:10.1679/aohc1950.41.267

Cited by 507 publications

(375 citation statements)

References 10 publications

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“…29) within decellularized lung sections, and examined collagen fibre microarchitecture using birefringence microscopy 29,30 . In control lungs, collagen fibres aligned with the alveolar septae, whereas they appeared to be more randomly distributed in BAPN-treated lungs (Fig.…”

Section: Resultsmentioning

confidence: 99%

Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics

et al. 2013

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Increased vascular permeability contributes to many diseases, including acute respiratory distress syndrome, cancer and inflammation. Most past work on vascular barrier function has focused on soluble regulators, such as tumour-necrosis factor-a. Here we show that lung vascular permeability is controlled mechanically by changes in extracellular matrix structure. Our studies reveal that pulmonary vascular leakage can be increased by altering extracellular matrix compliance in vitro and by manipulating lysyl oxidase-mediated collagen crosslinking in vivo. Either decreasing or increasing extracellular matrix stiffness relative to normal levels disrupts junctional integrity and increases vascular leakage. Importantly, endotoxin-induced increases of vascular permeability are accompanied by concomitant increases in extracellular matrix rigidity and lysyl oxidase activity, which can be prevented by inhibiting lysyl oxidase activity. The identification of lysyl oxidase and the extracellular matrix as critical regulators of lung vascular leakage might lead to the development of new therapeutic approaches for the treatment of pulmonary oedema and other diseases caused by abnormal vascular permeability.

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Section: Resultsmentioning

confidence: 99%

Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics

et al. 2013

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“…O método de Picrosirius, juntamente com a microscopia de polarização, demonstra com mais nitidez as fibras colágenas. [21][22][23][24][25] No trabalho observou-se que a quantidade de colágeno das pregas vocais submetidas aos diferentes tipos de cirurgia, tanto com laser de CO 2 quanto com bisturi com lâmina a frio, apresentava diferença significantemente estatística, como observado nas Tabelas 1 e 2. A presença de colágeno foi significantemente maior nas pregas vocais submetidas a incisões com bisturi com lâmina a frio do que com laser de CO 2 , o que permite concluir que o impacto causado na produção da voz é menor em pacientes submetidos a cirurgia com laser quando utilizados baixa potência e um microspot pequeno.…”

Section: Discussionunclassified

Estudo comparativo histológico na prega vocal após incisão com instrumental a frio e com laser de CO2 em modelo animal

Santos¹,

Grellet

Aguilar

et al. 2003

Rev. Bras. Otorrinolaringol.

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Dent re os vários tipos de laser usados em Medicina, o laser de CO 2 é o mais usado na Otorrinolaringologia e Cirurgia de Cabeça e Pescoço. As vantagens de seu uso são a diminuição do sangramento, a diminuição do edema no pós-operatório e a facilidade de acesso ao campo operatório, entre outras. Desde os trabalhos de Jako e Strong em 1972 1,2 , quando o laser de CO 2 passou a ser usado no tratamento de papilomatose laríngea e de lesões malignas glóticas iniciais, suas indicações têm aumetado, principalmente em lesões benignas, a partir da alta tecnologia desenvolvida dos últimos anos como, por exemplo, a diminuição do microspot e o uso do superpulso, reduzindo conseqüentemente seu efeito térmico sobre os tecidos. Métodos: Neste trabalho foram realizadas incisões com instrumental a frio e com laser de CO 2 1 watt de modo contínuo e superpulso, em pregas vocais caninas e observado, através de cortes histológicos corados pelo método de Sirius Red, a quantidade de colágeno depositada sobre as mesmas. Resultados: A quantidade de colágeno das pregas vocais foi maior do que no grupo controle, e estatisticamente maior no grupo de animais submetidos a procedimentos com instrumental a frio do que com laser de CO 2 . Não houve diferença estatística entre o grupo controle e o grupo submetido a incisões com instrumentos a frio. Conclusão: A microcirurgia de laringe com o laser de CO 2 , quando este é usado em baixa potência, com pequeno "microspot" e com superpulso, é um método seguro em relação à deposição de colágeno, quando comparado com instrumentos com lâmina a frio, obedecendo os princípios da fonomicrocirurgia. Among the several types of lasers employed in medicine today, the CO 2 is the most widely used in otorhinolaryngology and head and neck surgeries. The advantages of its use are: the reduction in bleeding, reduction in post-operative edema and the easy access to the operation site, among others. Ever since Jako and Strong's works in 1972 1,2 , when CO 2 was first applied in the treatment of papilomatosis and early glottic malign lesions, its indications have risen, most especially in benign lesions, due to new technology developed over the last years as, for example, the reduction of microspot and the superpulse, reducing the thermal effects on the tissues. Methods: In this work, incisions with cold steel instruments and 1watt continuous mode and super-pulse CO 2 laser were conducted on canine vocal folds. The amount of collagen deposited over the vocal folds was observed through histological exams using the Sirius Red method. Results: The amount of collagen on the vocal folds was greater than the control group and statistically greater in the group of animals submitted to surgical procedures using cold instruments than procedures involving CO 2 . There was no statistical difference between the control group and the group submitted to incisions with cold steel instruments. Conclusions:The larynx surgery with CO 2 laser, when employed in low potency, with small microspot and superpulse, is a safe method in relati...

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“…Previously, histochemical techniques have been described which were thought to enable a differentiation between collagen types I and III following the staining of paraffin sections with Picro-Sirius-Red Supra [4,5] or with Solophenyl-Red 3 BL [13]. The effect of these histochemical reactions was based on the assumption that collagen type I builds "thick" bundles which fluoresce red-orange in polarization microscopy, while collagen type III represents reticular fibers flourescing green under polarized light.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, in addition to the specific immunohistochemical method, a histochemical polarization technique has been described which seemed to allow differentiation between collagen types I and III in paraffin sections [4,5,13]. In our present study we compared the Solophenyl-Red 3 BL method described by Ogbuihi et al [13] with the immunohistochemical localization of collagen type III in formalin fixed and paraffin embedded tissue to analyze the specificity of this histochemical polarization technique for visualizing collagen subtypes.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the solophenyl-red polarization method and the immunohistochemical analysis for collagen type III

et al. 1992

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Summary.In the present study, we have compared the staining pattern of the Solophenyl-Red 3 BL-method for the visualization of collagen type III with the immunohistochemical staining in serial sections from 7 skin wounds (wound age 3 days up to 4 weeks) to elucidate the specifity of the histochemical staining method. Large amounts of collagen type III were clearly detectable in the investigated wounds using the immunohistochemical technique. In the sections stained with Solophenyl-Red, however, only 3 out of 7 skin lesions showed a significant positive red staining at the wound margin or in the granulation tissue, while the adjacent normal connective tissue revealed a typical intensive staining. Using polarization microscopy no characteristic bright green fibrils, as reported for collagen type III, could be seen in the wound areas without positive Solophenyl-Red staining. Since the localization of collagen type III detected by immunohistochemistry and the presumed distribution of this collagen type by the Solophenyl-Red method was not identical, the histochemical polarization method has to be regarded as non-specific for visualization of this collagen type.

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Differential Staining of Collagens Type I, II and III by Sirius Red and Polarization Microscopy

Cited by 507 publications

References 10 publications

Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics

Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics

Estudo comparativo histológico na prega vocal após incisão com instrumental a frio e com laser de CO2 em modelo animal

Comparison of the solophenyl-red polarization method and the immunohistochemical analysis for collagen type III

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