Differential Stem Proteomics and Metabolomics Profiles for Four Wheat Cultivars in Response to the Insect Pest Wheat Stem Sawfly

Lavergne, Florent D; Broeckling, Corey D.; Brown, Kitty; Cockrell, Darren M; Haley, Scott D.; Peairs, Frank B.; Pearce, Stephen; Wolfe, Lisa M.; Jahn, Courtney E.; Heuberger, Adam L.

doi:10.1021/acs.jproteome.9b00561

Cited by 14 publications

(12 citation statements)

References 77 publications

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“…As a major adverse environmental factor, salt stress greatly affects crop yields worldwide [ 34 ]. Crops have evolved elaborate regulatory mechanisms to manage salt stress, including modifications to protein composition and regulatory activities at the overall level as well as in changes in metabolites [ 35 , 36 ]. In this study, the alteration of proteins and metabolites of two types of barley seeds with different salt tolerances was studied after germination for 24 h under salt stress.…”

Section: Discussionmentioning

confidence: 99%

Combined Proteomic and Metabolomic Analysis of the Molecular Mechanism Underlying the Response to Salt Stress during Seed Germination in Barley

Chen

Wang

Yao

et al. 2022

IJMS

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Salt stress is a major abiotic stress factor affecting crop production, and understanding of the response mechanisms of seed germination to salt stress can help to improve crop tolerance and yield. The differences in regulatory pathways during germination in different salt-tolerant barley seeds are not clear. Therefore, this study investigated the responses of different salt-tolerant barley seeds during germination to salt stress at the proteomic and metabolic levels. To do so, the proteomics and metabolomics of two barley seeds with different salt tolerances were comprehensively examined. Through comparative proteomic analysis, 778 differentially expressed proteins were identified, of which 335 were upregulated and 443 were downregulated. These proteins, were mainly involved in signal transduction, propanoate metabolism, phenylpropanoid biosynthesis, plant hormones and cell wall stress. In addition, a total of 187 salt-regulated metabolites were identified in this research, which were mainly related to ABC transporters, amino acid metabolism, carbohydrate metabolism and lipid metabolism; 72 were increased and 112 were decreased. Compared with salt-sensitive materials, salt-tolerant materials responded more positively to salt stress at the protein and metabolic levels. Taken together, these results suggest that salt-tolerant germplasm may enhance resilience by repairing intracellular structures, promoting lipid metabolism and increasing osmotic metabolites. These data not only provide new ideas for how seeds respond to salt stress but also provide new directions for studying the molecular mechanisms and the metabolic homeostasis of seeds in the early stages of germination under abiotic stresses.

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Section: Discussionmentioning

confidence: 99%

Combined Proteomic and Metabolomic Analysis of the Molecular Mechanism Underlying the Response to Salt Stress during Seed Germination in Barley

Chen

Wang

Yao

et al. 2022

IJMS

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“…Proteomics is still an essential tool for studying insect morphological development and pest management at this stage. Recent research in insect proteomics shows that multiple modifications are associated with disease transmission and host defense mechanisms [ 50 – 53 ]. PTMs are involved in the conversion of normally inactive precursor proteins to mature proteins, and they affect the structure and function of a wide variety of proteins [ 54 ], Numerous protein modifications are required for the morphological development and disease transmission processes of insects [ 46 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Proteome-wide analysis of lysine 2-hydroxyisobutyrylation in Frankliniella occidentalis

et al. 2022

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Background Lysine 2-hydroxyisobutyrylation (Khib) is a novel and conserved post-translational modification (PTM). Frankliniella occidentalis are economically important agricultural pests globally and also notorious for vectoring destructive plant viruses. To better study the disease transmission mechanism of F. occidentalis, it is necessary to conduct in-depth analysis of it. So far, no Khib modification of insects has been reported. Results In this study, a proteome-wide analysis of Khib modifications in F. occidentalis was analyzed for the first time through the combination of high performance liquid chromatography fractionation technology and 2-hydroxyisobutyrylated peptide enrichment and other advanced technologies, 4093 Khib sites were identified on 1125 modified proteins. Bioinformatics and functional enrichment analyses showed that Khib-modified proteins were significantly enriched in many cell compartments and pathways, especially related to various cellular components and biological processes, and were more concentrated in ribosomes and proteasome subunits, involved in energy metabolism, protein synthesis and degradation, compared to the other nine species including Japonica rice, Homo sapiens, P. patens, Botrytis, Ustilaginoidea virens, Saccharomyces cerevisiae, T. gondii, C. albicans, and F. oxysporum. And Khib sites on virus-interacting insect proteins were discovered for the first time, such as cyclophilin and endoCP-GN. Conclusions After three repeated experiments, we found a total of 4093 Khib sites on 1125 proteins. These modified proteins are mainly concentrated in ribosomes and proteasome subunits, and are widely involved in a variety of critical biological activities and metabolic processes of F. occidentalis. In addition, for the first time, Khib modification sites are found on the proteome of F. occidentalis, and these sites could be acted as for the virus interaction, including cyclophilin and endoCP-GN. The global map of 2-hydroxyisobutyrylation in thrips is an invaluable resource to better understand the biological processes of thrips and provide new means for disease control and mitigation of pest damage to crops.

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“…One compound was identified as a 1-hydroxy-2-unsubstituted benzenoid. Benzenoids have previously been shown to increase in response to herbivory ( Lavergne et al. 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Volatile benzenoids can attract natural enemies of the herbivores ( Liu et al. 2018 , Lavergne et al. 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Seasonal and elevational variability in the induction of specialized compounds from mountain birch (Betula pubescens var. pumila) by winter moth larvae (Operophtera brumata)

Ryde

Rieksta

et al. 2021

Tree Physiology

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The mountain birch (Betula pubescens var. pumila (L.)) forest in the Subarctic is periodically exposed to insect outbreaks, which are expected to intensify due to climate change. To mitigate abiotic and biotic stresses, plants have evolved chemical defenses, including volatile organic compounds (VOCs) and non-volatile specialized compounds (NVSCs). Constitutive and induced production of these compounds, however, are poorly studied in Subarctic populations of mountain birch. Here, we assessed the joint effects of insect herbivory, elevation, and season on foliar VOC emissions and NVSC contents of mountain birch. VOCs were sampled in situ by an enclosure technique and analyzed by gas chromatography–mass spectrometry. NVSCs were analyzed by liquid chromatography–mass spectrometry using an untargeted approach. At low elevation, experimental herbivory by winter moth larvae (Operophtera brumata) increased emissions of monoterpenes and homoterpenes over the three-week feeding period, and sesquiterpenes and green leaf volatile in the end of the feeding period. At high elevation, however, herbivory augmented only homoterpene emissions. The more pronounced herbivory effects at low elevation were likely due to higher herbivory intensity. Of the individual compounds, linalool, ocimene, 4,8-dimethylnona-1,3,7-triene, 2-methyl butanenitrile, and benzyl nitrile were among the most responsive compounds in herbivory treatments. Herbivory also altered foliar NVSC profiles at both low and high elevations, with the most responsive compounds likely belonging to fatty acyl glycosides and terpene glycosides. Additionally, VOC emissions from non-infested branches were higher at high than low elevation, particularly during the early season, which was mainly driven by phenological differences. VOC emissions varied substantially over the season, largely reflecting the seasonal variations in temperature and light levels. Our results suggest that if insect herbivory pressure continues to rise in the mountain birch forest with ongoing climate change, it will significantly increase VOC emissions with important consequences for local trophic interactions and climate.

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Differential Stem Proteomics and Metabolomics Profiles for Four Wheat Cultivars in Response to the Insect Pest Wheat Stem Sawfly

Cited by 14 publications

References 77 publications

Combined Proteomic and Metabolomic Analysis of the Molecular Mechanism Underlying the Response to Salt Stress during Seed Germination in Barley

Combined Proteomic and Metabolomic Analysis of the Molecular Mechanism Underlying the Response to Salt Stress during Seed Germination in Barley

Proteome-wide analysis of lysine 2-hydroxyisobutyrylation in Frankliniella occidentalis

Seasonal and elevational variability in the induction of specialized compounds from mountain birch (Betula pubescens var. pumila) by winter moth larvae (Operophtera brumata)

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