Differential Surface Properties and Iron Distribution of <i>Acidianus manzaensis</i> YN25 Grown on Four Different Energy Substrates

Abstract

The surface properties and iron distribution of Acidianus manzaensis YN25 grown on four different energy substrates (chalcopyrite, pyrite, S0, and Fe2+) were comparatively studied. The results showed different growth and absorption features of A. manzaensis grown on different energy substrates. Results also showed that Zeta potentials, adsorption forces and cell surface acid-base properties were significantly influenced by the growth condition. Studies based on FT-IR and UV-vis spectroscopy indicated that the differences in cell surface properties may result from the different amounts of proteins and iron distribution on the cell surface of A. manzaensis grown on different growth conditions. The SR-μ-STXM images showed that the cell surface densities of iron spread on the cells grown on chalcopyrite, pyrite, S0 or Fe2+ were 4.31×10-5 - 23.25×10-5 g/cm2, 4.43×10-5 - 20.24×10-5 g/cm2, 0.10×10-5 - 0.29×10-5 g/cm2, and 6.45×10-5 - 24.06×10-5 g/cm2, respectively, further indicating that the surface of A. manzaensis cells grown on chalcopyrite, pyrite and Fe2+ had an iron-contained compounds, which might be the reason why the surface of A. manzaensis cells grown on chalcopyrite, pyrite and Fe2+ carried weak positive charges at pH 2 while negative for the cells grown on S0.