Differentially expressed angiogenic genes in diabetic erectile tissue — Results from a microarray screening

Castela, Ângela; Soares, Raquel; Rocha, Francisco Costa da; Medeiros, Rui; Ribeiro, Ricardo; Monteiro, Cátia; Gomes, Pedro; Vendeira, Pedro; Virag, R; Costa, Carla

doi:10.1016/j.ymgme.2011.11.002

Cited by 14 publications

(13 citation statements)

References 28 publications

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“…It regulates the proliferation and differentiation of a number of cell types by activating the type 1 insulin‐like growth factor receptor (IGF‐1R). The decreased expression of IGF‐1 in corporal tissues was associated with ED (Pu et al ., , ; Castela et al ., ). Bochinski et al ., () demonstrated that the intraperitoneal injection of IGF‐1 enhanced erectile function by facilitating the regeneration of nitric oxide synthase (NOS)‐containing fibers in a rat model of bilateral cavernous nerve cryoablation.…”

Section: Discussionmentioning

confidence: 97%

Adipose‐derived stem cells improve erectile function partially through the secretion of IGF‐1, bFGF, and VEGF in aged rats

Yang

Zhang

Zang³

et al. 2018

Andrology

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Adipose-derived stem cells (ADSCs) have recently been considered as a promising therapy for erectile dysfunction (ED). However, the mechanism of ADSC-based therapy is unclear. Insulin-like growth factor-1 (IGF-1), basic fibroblast growth factor (bFGF), and vascular endothelial growth factor (VEGF) secreted by ADSCs were assessed in vitro. Sixteen 24-month-old male Sprague-Dawley rats were used for comparative analysis of 2-week treatment with labeled ADSCs or PBS. Eight additional 5-month-old rats were used as a young rat group. At 2 weeks post-transplantation, all rats were analyzed for erectile function, cavernous IGF-1, bFGF and VEGF levels, and penile histology. Conditioned medium and co-culture systems were used in cell experiments to detect how growth factors act on corpus cavernosum smooth muscle cells (CCSMCs) under oxidative stress conditions via crystal violet staining and immunofluorescence staining. We found that ADSCs secreted significantly higher IGF-1, bFGF, and VEGF levels in culture medium compared with basal medium. Compared with young rats, untreated aged rats had significantly lower Max ICP/MAP and ADSC treatment significantly increased the ratio. Immunofluorescence staining demonstrated a small number of labeled ADSCs in the corpus cavernosum. The untreated aged rats showed significantly decreased cavernous IGF-1, bFGF, and VEGF levels and significantly decreased contents of cavernous smooth muscle and endothelium compared with young rats. ADSC treatment partially normalized these alterations. In cell experiments, the groups receiving growth factor neutralizing antibody separately or combined had significantly decreased numbers of CCSMCs compared with control groups. These results indicated that ADSC treatment may improve aging-related ED partially through the secretion of IGF-1, bFGF, and VEGF.

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Section: Discussionmentioning

confidence: 97%

Adipose‐derived stem cells improve erectile function partially through the secretion of IGF‐1, bFGF, and VEGF in aged rats

Yang

Zhang

Zang³

et al. 2018

Andrology

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“…Many researches have manifested various molecular mechanisms in the process of A-ED, such as apoptosis, fibrosis, and inflammation [6,27]. Also, many genes have been investigated to be involved in this process, including IGF1, TGFB2, END1, CAV1, SIRT1, BDNF, and so on [16][17][18][27][28][29]. Actually, these genes are regulated by many miRNAs at transcriptional level [10,30].…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of the MicroRNA Profile in Aging Rats with Erectile Dysfunction

Pan

Zhang

Qiu

et al. 2014

The Journal of Sexual Medicine

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“…In addition, the role of glycemic control on EPCs functions and its effects on EPCs modulators has never been assessed in diabetes‐associated ED. We aimed to study in an ED‐prone experimental model of diabetes [Jin et al, ; Castela et al, ], the role of insulin therapy on EPCs production/mobilization, on testosterone levels, and its impact in the systemic and penile expression of SDF‐1α.…”

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confidence: 99%

Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control?

Castela

Gomes

Silvestre

et al. 2016

J of Cellular Biochemistry

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Erectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endothelial dysfunction (EDys) and hampering repair mechanisms. However, scarce information is available linking vasculogenesis mediated by Endothelial Progenitor Cells (EPCs) and diabetes-associated ED. Furthermore, it remains to be elucidated if glycemic control plays a role on EPCs functions, EPCs modulators, and penile vascular health. We evaluated the effects of diabetes and insulin therapy on bone marrow (BM) and circulating EPCs, testosterone, and systemic/penile Stromal Derived Factor-1 alpha (SDF-1α) expression. Male Wistar rats were divided into groups: age-matched controls, 8-weeks streptozotocin-induced type 1 diabetics, and insulin-treated 8-weeks diabetics. EPCs were identified by flow cytometry for CD34/CD133/VEGFR2/CXCR4 antigens. Systemic SDF-1α and testosterone levels were evaluated by ELISA. Penile SDF-1α protein expression was assessed, in experimental and human diabetic cavernosal samples, by immunohistochemical techniques. Diabetic animals presented a reduction of BM-derived EPCs and an increase in putative circulating endothelial cells (CECs) sloughed from vessels wall. These alterations were rescued by insulin therapy. In addition, glycemic control promoted an increase in systemic testosterone and SDF-1α levels, which were significantly decreased in animals with diabetes. SDF-1α protein expression was reduced in experimental and human cavernosal diabetic samples, an effect prevented by insulin in treated animals. Insulin administration rescued the effects of diabetes on BM function, CECs levels, testosterone, and plasmatic/penile SDF-1α protein expression. This emphasizes the importance of glycemic control in the prevention of diabetes-induced systemic and penile EDys, by the amelioration of endothelial damage, and increase in protective pathways. J. Cell. Biochem. 118: 82-91, 2017. © 2016 Wiley Periodicals, Inc.

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Differentially expressed angiogenic genes in diabetic erectile tissue — Results from a microarray screening

Cited by 14 publications

References 28 publications

Adipose‐derived stem cells improve erectile function partially through the secretion of IGF‐1, bFGF, and VEGF in aged rats

Adipose‐derived stem cells improve erectile function partially through the secretion of IGF‐1, bFGF, and VEGF in aged rats

Identification and Characterization of the MicroRNA Profile in Aging Rats with Erectile Dysfunction

Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control?

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