Differentially expressed circular RNAs in maternal and neonatal umbilical cord plasma from SGA compared with AGA

Wang, Yan; Li, Shufang; Dang, Yujiao; Shi, Xiaoming; Chen, Lian; Wang, Nan; Cai, Yuanheng; Zhao, Yangyu

doi:10.1002/jcb.29317

Cited by 6 publications

(5 citation statements)

References 37 publications

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“…Unfortunately, the biological roles of hsa_circ_0001360 are currently very little studied. Only a recent study [33] reported that hsa_circ_0001360 was markedly down‐regulated in the placental tissue and plasma of small‐for‐gestational‐age fetuses compared with appropriate‐for‐gestational‐age fetuses and had two target miRNAs (hsa_miR_4725_3p and hsa_miR_671_5p) that were significantly elevated in placental tissue of small‐for‐gestational‐age fetuses. Interestingly, hsa_miR_4725_3p was observed to be up‐regulated in patients with glaucoma [34] and was involved in xanthohumol‐attenuated glioma cell invasion [35] and cotreatment of melatonin‐ and pterostilbene‐induced apoptosis of colorectal cancer cells [36].…”

Section: Discussionmentioning

confidence: 99%

“…In additional, Verduci et al [32] reported that the Unfortunately, the biological roles of hsa_circ_0001360 are currently very little studied. Only a recent study [33] reported that hsa_circ_0001360 were markedly downregulated in the placental tissue and plasma of small for gestational age (SGA) fetuses compared to appropriate for gestational age fetuses, and had two target miRNAs (hsa_miR_4725_3p and hsa_miR_671_5p) that were significantly elevated in placental tissue of SGA fetuses. Interestingly, hsa_miR_4725_3p was observed to be upregulated in patients with glaucoma [34], and was involved in…”

Section: Prediction Of Circrna-mirna-mrna Interaction Network Of Hsa_mentioning

confidence: 99%

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Circular RNA profiles and the potential involvement of down‐expression of hsa_circ_0001360 in cutaneous squamous cell carcinogenesis

Chen

Huang

et al. 2021

FEBS Open Bio

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Circular RNAs (circRNAs) act as sponges of non-coding RNAs (ncRNAs) and have been implicated in many pathophysiological processes, including tumor development and progression. However, their roles in cutaneous squamous cell carcinoma (cSCC) are not yet well understood. The present study aimed to identify differentially expressed circRNAs and their potential functions in cutaneous squamous cell carcinogenesis. The expression profiles of circRNAs in 3 paired cSCC and adjacent This article is protected by copyright. All rights reserved Accepted Article 4 non-tumorous tissues were detected with RNA sequencing and bioinformatics analysis. The candidate circRNAs were validated by PCR, Sanger sequencing, and qRT-PCR in another 5 matched samples. The biological functions of circRNAs in SCL-1 cells were assessed using circRNA silencing and overexpression, MTS, flow cytometry, transwell and colony formation assays. In addition, the circRNA-miRNA-mRNA interaction networks were predicted by bioinformatics. In summary, 1115 circRNAs, including 457 up-and 658 down-regulated circRNAs (fold change ≥ 2 and p < 0.05), were differentially expressed in cSCC compared with adjacent non-tumorous tissues. Of 4 selected circRNAs, 2 circRNAs (hsa_circ_0000932 and hsa_circ_0001360) were confirmed to be significantly decreased in cSCC using PCR, Sanger sequencing, and qRT-PCR. Furthermore, hsa_circ_0001360 silencing was found to result in a significant increase of the proliferation, migration and invasion but a significant decrease of apoptosis in SCL-1 cells in vitro, whereas hsa_circ_0001360 overexpression showed the opposite regulatory effects. Hsa_circ_0001360 was predicted to interact with 5 miRNAs and their corresponding genes. In conclusion, circRNA dysregulation may play a critical role in carcinogenesis of cSCC, and hsa_circ_0001360 may have potential as a biomarker for cSCC.

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Section: Discussionmentioning

confidence: 99%

Section: Prediction Of Circrna-mirna-mrna Interaction Network Of Hsa_mentioning

confidence: 99%

Circular RNA profiles and the potential involvement of down‐expression of hsa_circ_0001360 in cutaneous squamous cell carcinogenesis

Chen

Huang

et al. 2021

FEBS Open Bio

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“…Upregulated hsa_circ_0089172 expression was confirmed using RT-qPCR, whilst in vitro experiments revealed that hsa_circ_0089172 could be a potential diagnostic biomarker, as it plays a crucial role in the pathogenesis of Hashimoto's thyroiditis by sponging miR-125a-3p. Zhao et al ( 37 ), Qian et al ( 38 ) and Wang et al ( 39 ) assessed differences in the circRNA expression level of peripheral blood samples from patients with community-acquired pneumonia, active pulmonary tuberculosis, and in patients who were small for their gestational age, respectively, with their results suggesting that circRNAs could be reliable biomarkers for diagnosing these conditions. Notably, to the best of our knowledge, the effects of aberrant circRNA expression on ISS has not been investigated, even though an increasing number of studies have investigated the functions of circRNAs in diseases ( 34 - 39 ).…”

Section: Discussionmentioning

confidence: 99%

Hsa_circularRNA_0079201 suppresses chondrocyte proliferation and endochondral ossification by regulating the microRNA‑140‑3p/SMAD2 signaling pathway in idiopathic short stature

et al. 2020

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Circular (circ)RNAs are an important group of non-coding RNAs involved in different pathological and physiological functions, such as longitudinal bone growth. However, the effects of an increase or decrease in circRNA expression on idiopathic short stature (ISS) remain largely unknown. The present study compared the circRNA expression patterns of patients with ISS and healthy individuals to identify differentially expressed circRNAs involved in the regulation of ISS pathogenesis and their target microRNAs (miR). Microarray analysis revealed that 145 circRNAs were differentially expressed in patients with ISS, including 83 up- and 62 downregulated circRNAs. Reverse transcription-quantitative PCR confirmed that hsa_circRNA_0079201 was increased in patients with ISS compared with that in the normal individuals, whilst hsa_circRNA_0079201 overexpression in human chondrocytes was shown to significantly suppress their proliferation, hypertrophy and endochondral ossification abilities. Luciferase reporter assays identified that circRNA_0079201 acted as an miR-140-3p sponge. In situ hybridization confirmed the co-localization of circRNA_0079201 and miR-140-3p in the human chondrocyte and neonatal femur growth plate of C57 mice, while rescue experiments demonstrated that miR-140-3p overexpression reversed the inhibition of human chondrocyte proliferation, hypertrophy and endochondral ossification, caused by circRNA_0079201 overexpression. Bioinformatics analysis and luciferase reporter assays revealed that SMAD2 was a potential target gene of miR-140-3p. Furthermore, overexpressing circRNA_0079201 in human chondrocytes suppressed miR-140-3p and increased SMAD2 protein expression level. Taken together, chondrocyte proliferation, hypertrophy and endochondral ossification in ISS was suppressed by a novel regulatory axis consisting of the hsa_circRNA_0079201/miR-140-3p/SMAD2 pathway. The present study provided evidence that hsa_circRNA_0079201 may be a potential target for ISS therapy.

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“…CircRNAs have a clear function of acting as miRNA sponges [23]. The cCDR1as/miR-7 signal axis has been investigated in many diseases [24,25,26].…”

Section: Discussionmentioning

confidence: 99%

Circular RNAs are abundant and dynamically expressed during the embryonic lung development of C57BL/6 mice

Zhang

Yin

et al. 2020

Heliyon

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Circular RNAs (circRNAs), a novel type of endogenous RNAs, can function as microRNA (miRNA) sponges capable of regulating gene transcription, binding to RNA-associated proteins, and even encoding proteins. CircRNAs are involved in various cell behaviors, such as proliferation and apoptosis. The mouse model has also been demonstrated to be similar to that of humans in many studies. To explore the profile of circRNAs during embryonic lung development and their potential functions in lung development-related diseases, mouse embryos at the pseudoglandular phase, canalicular phase, saccular phase, and alveolar phase were collected. High-throughput sequencing was then used to identify a total of 1,735 circRNAs (junction reads !5 and p < 0.05). It is well known that the functions of circRNAs are related to host genes. In our study, bioinformatics analysis indicated that the screened host genes were closely associated with lung development and included the Hippo signaling pathway, PI3K-Akt signaling pathways, and TGF-β signaling pathways. Moreover, miRNA sponges are another mechanism involved in lung development. Therefore, we predicted many miRNAs binding to circRNAs, such as miR-17 and miR-20, using the TargetScan and miRanda databases. Previously, miRNAs were proven to be necessary for lung development. The peak expression of circRNAs is distributed at different time points, suggesting their involvement in different stages of embryonic mouse lung development.

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Differentially expressed circular RNAs in maternal and neonatal umbilical cord plasma from SGA compared with AGA

Cited by 6 publications

References 37 publications

Circular RNA profiles and the potential involvement of down‐expression of hsa_circ_0001360 in cutaneous squamous cell carcinogenesis

Circular RNA profiles and the potential involvement of down‐expression of hsa_circ_0001360 in cutaneous squamous cell carcinogenesis

Hsa_circularRNA_0079201 suppresses chondrocyte proliferation and endochondral ossification by regulating the microRNA‑140‑3p/SMAD2 signaling pathway in idiopathic short stature

Circular RNAs are abundant and dynamically expressed during the embryonic lung development of C57BL/6 mice

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