Differentially expressed galactinol synthase(s) in chickpea are implicated in seed
vigor and longevity by limiting the age induced ROS accumulation

Salvi, Prafull; Saxena, Saurabh C.; Petla, Bhanu Prakash; Kamble, Nitin Uttam; Kaur, Harmeet; Verma, Pooja; Rao, Venkateswara; Ghosh, Shraboni; Majee, Manoj

doi:10.1038/srep35088

Cited by 83 publications

(68 citation statements)

References 68 publications

(84 reference statements)

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“…3B ). RFOs accumulate during seeds development is thought to be important for desiccation tolerance during seed maturation and longevity in dehydrated state 18 , 39 . Seeds of the AtRS4 and AtRS5 double mutant showed a total loss of RFOs and five days delayed germination phenotype in darkness, suggesting that RFOs also act as a galactose store in seeds and are necessary for rapid germination in the dark 40 .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and expression analyses of genes involved in raffinose accumulation in sesame

You

Wang

Zhang

et al. 2018

Sci Rep

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Sesame (Sesamum indicum L.) is an important oilseed crop. However, multiple abiotic stresses severely affect sesame growth and production. Raffinose family oligosaccharides (RFOs), such as raffinose and stachyose, play an important role in desiccation tolerance of plants and developing seeds. In the present study, three types of key enzymes, galactinol synthase (GolS), raffinose synthase (RafS) and stachyose synthase (StaS), responsible for the biosynthesis of RFOs were identified at the genome-wide scale in sesame. A total of 7 SiGolS and 15 SiRS genes were identified in the sesame genome. Transcriptome analyses showed that SiGolS and SiRS genes exhibited distinct expression profiles in different tissues and seed developmental stages. Comparative expression analyses under various abiotic stresses indicated that most of SiGolS and SiRS genes were significantly regulated by drought, osmotic, salt, and waterlogging stresses, but slightly affected by cold stress. The up-regulation of several SiGolS and SiRS genes by multiple abiotic stresses suggested their active implication in sesame abiotic stress responses. Taken together, these results shed light on the RFOs-mediated abiotic stress resistance in sesame and provide a useful framework for improving abiotic stress resistance of sesame through genetic engineering.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification and expression analyses of genes involved in raffinose accumulation in sesame

You

Wang

Zhang

et al. 2018

Sci Rep

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“…There are some publications suggesting that galactinol positively regulates seed vigor in A. thaliana and some other plant species (Salvi et al, 2016;de Souza Vidigal et al, 2016). Galactinol was considered as a marker for seed storability in A. thaliana, cabbage, and tomato (de Souza Vidigal et al, 2016), and overexpression of the chickpea GALACTINOL SYNTHASE (CaGOLS) gene in A. thaliana increased the galactinol and raffinose content and enhanced seed vigor (Salvi et al, 2016). Since both galactinol and raffinose were increased by overexpression of the CaGOLS gene, it is not clear whether galactinol, raffinose, or both directly enhanced A. thaliana seed vigor.…”

Section: Total Rfo Amounts and The Ratio Of Total Rfos To Sucrose Bumentioning

confidence: 99%

“…In addition, galactinol and raffinose are substrates for synthesis of HDP RFOs, such as stachyose or verbascose. The change of stachyose or verbascose and their effects on A. thaliana seed vigor was not investigated in CaGOLS expressing A. thaliana (Salvi et al, 2016). This information is important because our evidence from overexpressing one or more genes whose products are involved in RFO biosynthesis suggest that the total amount of RFOs and the ratio of RFOs/ sucrose, but not galactinol, are responsible for A. thaliana seed vigor (Table 1 and Figure 6B).…”

Section: Total Rfo Amounts and The Ratio Of Total Rfos To Sucrose Bumentioning

confidence: 99%

“…Tetrazolium assay was performed following a published protocol with modification (Salvi et al, 2016). Seeds treated with or without artificial aging were initially sterilized with 10% hypochlorous acid (containing 0.03% Tween-20) for 5 min and then washed in sterilized distilled water five times.…”

Section: Tetrazolium Assay Of the Arabidopsis Thaliana Seed Viabilitymentioning

confidence: 99%

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Regulation of Seed Vigor by Manipulation of Raffinose Family Oligosaccharides in Maize and Arabidopsis thaliana

et al. 2017

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Raffinose family oligosaccharides (RFOs) accumulate in seeds during maturation desiccation in many plant species. However, it remains unclear whether RFOs have a role in establishing seed vigor. GALACTINOL SYNTHASE (GOLS), RAFFINOSE SYNTHASE (RS), and STACHYOSE SYNTHASE (STS) are the enzymes responsible for RFO biosynthesis in plants. Interestingly, only raffinose is detected in maize seeds, and a unique maize RS gene (ZmRS) was identified. In this study, we found that two independent mutator (Mu)-interrupted zmrs lines, containing no raffinose but hyperaccumulating galactinol, have significantly reduced seed vigor, compared with null segregant controls. Unlike maize, Arabidopsis thaliana seeds contain several RFOs (raffinose, stachyose, and verbascose). Manipulation of A. thaliana RFO content by overexpressing ZmGOLS2, ZmRS, or AtSTS demonstrated that co-overexpression of ZmGOLS2 and ZmRS, or overexpression of ZmGOLS2 alone, significantly increased the total content of RFOs and enhanced Arabidopsis seed vigor. Surprisingly, while overexpression of ZmRS increased seed raffinose content, its overexpression dramatically decreased seed vigor and reduced the seed amounts of galactinol, stachyose, and verbascose. In contrast, the atrs5 mutant seeds are similar to those of the wild type with regard to seed vigor and RFO content, except for stachyose, which accumulated in atrs5 seeds. Total RFOs, RFO/sucrose ratio, but not absolute individual RFO amounts, positively correlated with A. thaliana seed vigor, to which stachyose and verbascose contribute more than raffinose. Taken together, these results provide new insights into regulatory mechanisms of seed vigor and reveal distinct requirement for RFOs in modulating seed vigor in a monocot and a dicot.

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“…Likewise, the abundance of HSP17.6I was positively correlated with seed performance in this species [74] and changes in enzyme activities in four Brassica species were proposed as indicators of seed deterioration [75]. Similarly, several studies have proposed addressed the use of novel seed quality markers, such as the production of methanol, ethanol or galactinol in stored seeds [11,12,[76][77][78]. However, the development of novel, equally reliable methods is always desirable.…”

Section: Discussionmentioning

confidence: 99%

Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seeds

Torre

Kaschani

Kaiser

et al. 2019

Biochemical Journal

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Seed quality is affected by different constituents of the seed. In general, seed lots are considered to be of high quality when they exhibit fast and homogeneous germination. When seeds are stored, they undergo different degrees of damage that have detrimental effects on their quality. Therefore, accurate prediction of the seed quality and viability levels of a seed lot is of high importance in the seed-producing industry. Here, we describe the use of activity-based protein profiling of proteases to evaluate the quality of artificially and naturally aged seeds of Arabidopsis thaliana. Using this approach, we have identified two protease activities with opposite behaviours in aged seeds of Arabidopsis that correlate with the quality status of the seeds. We show that vacuolar processing enzymes (VPEs) become more active during the ageing process, in both artificial and natural ageing treatments. Secondly, we demonstrate that serine hydrolases are active at the beginning of our artificial ageing treatment, but their labelling decreases along with seed viability. We present a list of candidate hydrolases active during seed germination and propose that these protease activities can be used in combination with VPEs to develop novel markers of seed quality.

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Differentially expressed galactinol synthase(s) in chickpea are implicated in seed vigor and longevity by limiting the age induced ROS accumulation

Cited by 83 publications

References 68 publications

Genome-wide identification and expression analyses of genes involved in raffinose accumulation in sesame

Genome-wide identification and expression analyses of genes involved in raffinose accumulation in sesame

Regulation of Seed Vigor by Manipulation of Raffinose Family Oligosaccharides in Maize and Arabidopsis thaliana

Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seeds

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