Differentially expressed genes in skeletal muscle tissues from castrated Qinchuan cattle males compared with those from intact males

Zhang, Y.-Y.; Liu, Zan; Wang, H.-B.; Qing, LU; Wu, Kaiyi; Quan, Song; Li, C.-Q.; Zhong, Xiaogang; Wang, C.-J.

doi:10.1016/j.livsci.2010.06.070

Cited by 13 publications

(8 citation statements)

References 47 publications

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“…COL16A1 is a gene suggested to have importance for cell adhesion 28 . COL4A3 was also identified as a structural component of collagen, which is known to affect meat tenderness.…”

Section: Discussionmentioning

confidence: 99%

“…COL4A3 was also identified as a structural component of collagen, which is known to affect meat tenderness. In turn, myosin is a major structural protein of the thick filament of the sarcomere 28 . A previous study demonstrated that MyHC isoform composition affects the quality of the meat originating from bovine muscles 29 and is a major determinant of the phenotypic properties of muscles 30 .…”

Section: Discussionmentioning

confidence: 99%

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Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing

Hao

Cui

2016

Sci Rep

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Heat stress affects muscle development and meat quality in food animals; however, little is known regarding its regulatory mechanisms at the epigenetic level, such as via DNA methylation. In this study, we aimed to compare the DNA methylation profiles between control and heat-stressed pigs to identify candidate genes for skeletal muscle development and meat quality. Whole-genome bisulfite sequencing was used to investigate the genome-wide DNA methylation patterns in the longissimus dorsi muscles of the pigs. Both groups showed similar proportions of methylation at CpG sites but exhibited different proportions at non-CpG sites. A total of 57,147 differentially methylated regions were identified between the two groups, which corresponded to 1,422 differentially methylated genes. Gene ontogeny and KEGG pathway analyses indicated that these were mainly involved in energy and lipid metabolism, cellular defense and stress responses, and calcium signaling pathways. This study revealed the global DNA methylation pattern of pig muscle between normal and heat stress conditions. The result of this study might contribute to a better understanding of epigenetic regulation in pig muscle development and meat quality.

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“…COL16A1 is a gene suggested to have importance for cell adhesion 28 . COL4A3 was also identified as a structural component of collagen, which is known to affect meat tenderness.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing

Hao

Cui

2016

Sci Rep

View full text Add to dashboard Cite

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“…All of these variances in the gene profile can provide explanation for the differences in the fiber types in skeletal muscle for different locations and breeds. A previous study identified MYH3 and MYH8 as differentially expressed genes between intact and castrated cattle [ 29 ], indicating that these two genes are major genes for muscle fiber properties. We believe that these genes (MYH7b, MYH15, MYL1, MYL3 and MYL10) partially contribute to the difference in skeletal muscle deposition between locations and duck breeds.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Profiling Identifies Location-Specific and Breed-Specific Differentially Expressed Genes in Embryonic Myogenesis in Anas Platyrhynchos

Zhang

Liu

et al. 2015

PLoS ONE

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Skeletal muscle growth and development are highly orchestrated processes involving significant changes in gene expressions. Differences in the location-specific and breed-specific genes and pathways involved have important implications for meat productions and meat quality. Here, RNA-Seq was performed to identify differences in the muscle deposition between two muscle locations and two duck breeds for functional genomics studies. To achieve those goals, skeletal muscle samples were collected from the leg muscle (LM) and the pectoral muscle (PM) of two genetically different duck breeds, Heiwu duck (H) and Peking duck (P), at embryonic 15 days. Functional genomics studies were performed in two experiments: Experiment 1 directly compared the location-specific genes between PM and LM, and Experiment 2 compared the two breeds (H and P) at the same developmental stage (embryonic 15 days). Almost 13 million clean reads were generated using Illumina technology (Novogene, Beijing, China) on each library, and more than 70% of the reads mapped to the Peking duck (Anas platyrhynchos) genome. A total of 168 genes were differentially expressed between the two locations analyzed in Experiment 1, whereas only 8 genes were differentially expressed when comparing the same location between two breeds in Experiment 2. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes pathways (KEGG) were used to functionally annotate DEGs (differentially expression genes). The DEGs identified in Experiment 1 were mainly involved in focal adhesion, the PI3K-Akt signaling pathway and ECM-receptor interaction pathways (corrected P-value<0.05). In Experiment 2, the DEGs were associated with only the ribosome signaling pathway (corrected P-value<0.05). In addition, quantitative real-time PCR was used to confirm 15 of the differentially expressed genes originally detected by RNA-Seq. A comparative transcript analysis of the leg and pectoral muscles of two duck breeds not only improves our understanding of the location-specific and breed-specific genes and pathways but also provides some candidate molecular targets for increasing muscle products and meat quality by genetic control.

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“…Beef quality may be assessed by intramuscular fat (IMF) deposition, as well as by marbling and fatty acid composition, which further determines meat palatability, including flavor, tenderness and juiciness [8][9][10]. Numerous trials regarding beef cattle breeds such as Bos indicus bulls, Qinchuan cattle, Chinese Simmental cattle and Korean cattle, have suggested that castration reduced meat yield, yet improved meat quality, including fatty acid composition and IMF content [11][12][13][14]. Two recent studies physicochemically characterized the beef quality of Holstein bulls, and the results suggested that the steer beef contained higher redness, intramuscular fat and soluble collagen, thus indicating a better eating quality than bull beef [15,16].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of longissimus lumborum in Holstein bulls and steers with different beef qualities

Wang

et al. 2020

PLoS ONE

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Previous research regarding Holstein cows has mainly focused on increasing milk yield. However, in order to maximize the economical profits of Holstein cattle farming, it is necessary to fully take advantage of Holstein bulls to produce high-grade beef. The present study aims to investigate different transcriptomic profiling of Holstein bulls and steers, via highthroughput RNA-sequencing (RNA-seq). The growth and beef quality traits of Holstein steers and bulls were characterized via assessment of weight, rib eye area, marbling score, shear force and intramuscular fat percentage of the longissimus lumborum (LL) muscle. The results indicated that castration improved the meat quality, yet reduced the meat yield. Subsequently, RNA-seq of the LL muscle from Holstein steers and bulls revealed a total of 56 differentially expressed genes (DEGs). We performed the functional enrichment analysis in Gene Ontology (GO) annotations of the DEGs using GOseq R package software and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis using KOBAS tool. Through the integrated analysis of DEGs with reported QTLs and SNPs, seven promising candidate genes potentially affecting the beef quality of LL muscle following castration were discovered, including muscle structural protein coding genes (MYH1, MYH4, MYH10) and functional protein coding genes (GADL1, CYP2R1, EEPD1, SHISA3). Among them, MYH10, GADL1, CYP2R1, EEPD1 and SHISA3 were novel candidate genes associated with beef quality traits. Notably, EEPD1 was associated with both meat quality and reproduction traits, thus indicating its overlapping role in responding to hormone change, and subsequently inducing beef quality improvement. Our findings provide a complete dataset of gene expression profile of LL in Holstein bulls and steers, and will aid in understanding how castration influence meat yield and quality.

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Differentially expressed genes in skeletal muscle tissues from castrated Qinchuan cattle males compared with those from intact males

Cited by 13 publications

References 47 publications

Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing

Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing

Transcriptional Profiling Identifies Location-Specific and Breed-Specific Differentially Expressed Genes in Embryonic Myogenesis in Anas Platyrhynchos

Transcriptome profiling of longissimus lumborum in Holstein bulls and steers with different beef qualities

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