Differentially expressed genes in the fat body of Bombyx mori in response to phoxim insecticide

Gu, Zhiya; Li, F.C.; Wang, B.B.; Xu, Kai; Ni, Min; Zhang, Hao; Shen, Wei; Li, B.

doi:10.1016/j.pestbp.2014.10.007

Cited by 31 publications

(9 citation statements)

References 41 publications

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“…A set of miRNAs were found to target several families of important genes that are often involved in insecticide resistance, such as cytochrome P45025262728, esterase29, GSTs30, ABC transporter family protein3132, cuticle protein33, glutamate-gated chloride channel3435 and superoxide dismutase (SOD)36. The target genes of some selected miRNAs are listed in Table 5.…”

Section: Resultsmentioning

confidence: 99%

Global identification of microRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

Zhu

Liu

et al. 2017

Sci Rep

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The diamondback moth (DBM), Plutella xylostella (L.), is one of the most serious cruciferous pests and has developed high resistance to most insecticides, including chlorantraniliprole. Previous studies have reported several protein-coding genes that involved in chlorantraniliprole resistance, but research on resistance mechanisms at the post-transcription level is still limited. In this study, a global screen of microRNAs (miRNAs) associated with chlorantraniliprole resistance in P. xylostella was performed. The small RNA libraries for a susceptible (CHS) and two chlorantraniliprole resistant strains (CHR, ZZ) were constructed and sequenced, and a total of 199 known and 30 novel miRNAs were identified. Among them, 23 miRNAs were differentially expressed between CHR and CHS, and 90 miRNAs were differentially expressed between ZZ and CHS, of which 11 differentially expressed miRNAs were identified in both CHR and ZZ. Using miRanda and RNAhybrid, a total of 1,411 target mRNAs from 102 differentially expressed miRNAs were predicted, including mRNAs in several groups of detoxification enzymes. The expression of several differentially expressed miRNAs and their potential targets was validated by qRT-PCR. The results may provide important clues for further study of the mechanisms of miRNA-mediated chlorantraniliprole resistance in DBM and other target insects.

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Section: Resultsmentioning

confidence: 99%

Global identification of microRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

Zhu

Liu

et al. 2017

Sci Rep

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“…However, those mainly involved in detoxification of organophosphate insecticide CEs are mediated nonspecifically (Cui et al, 2011;. Feeding on phoxim (Gu et al, 2015) and chlorantraniliprole (Mao et al, 2019) Recent studies have demonstrated that lipase from intestinal bacterium, in particular the lipase-producing bacterial strain Bacillus pumilus SW41, is a potential antiviral factor for silkworm against BmNPV (Liu et al, 2018). CEs and other protease-like hemocyte proteases, antitrypsin, and aminopeptidase N are higher in resistant silkworms compared to susceptible individuals (Guo et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

A Comparative Studies of the Activities of Phenoloxidase, Carboxylesterase and Alkaline Phosphatase from Different Geographic Strains of Flacherie-Infected Bombyx mori

Li¹,

Zhang²,

Feng³

2021

Agriculture

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“…Mao et al (2019) found that the activity of detoxification enzymes in the silkworm increased under exposure to CAP (chlorantraniliprole), and the actions of CYPs, GSTs, and CarEs were all upregulated (Mao et al, 2019). It has been observed in the silkworm's fat body that phoxim induces the upregulation of BmCYP6b29, BmGST4, and BmGSTo1, and these proteins are involved in detoxification (Gu et al, 2015). When the silkworm is exposed to pyriproxyfen, it will cause damage to the silk gland.…”

Section: Gsts Work With Other Detoxification Enzymes To Implement Detoxification Functionsmentioning

confidence: 99%

Construction and analysis of the protein–protein interaction network for the detoxification enzymes of the silkworm, Bombyx mori

Xin

Zhang

2021

Arch Insect Biochem Physiol

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Detoxification enzymes are necessary for insects to metabolize toxic substances and maintain physiological activities. Cytochromes P450 (CYPs), glutathione S‐transferases (GSTs), and carboxylesterase (CarEs) are the main detoxification enzymes in insects. In addition, UDP‐glucosyltransferase and ATP‐binding cassette transporter also participate in the process of material metabolism. This study collected proteins related to detoxification in the silkworm, Bombyx mori (Lepidoptera: Bombycidae). And we performed Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis on these proteins to understand their biological function. We constructed the protein–protein interaction network for the silkworm's detoxification enzymes and analyzed the network's topological properties. We found that BGIBMGA014046‐TA, BGIBMGA003221‐TA, BGIBMGA011092‐TA, BGIBMGA000074‐TA, and LOC732976 are the essential proteins in the network. These proteins are primarily involved in the process of ribosome biogenesis and may be related to protein synthesis. We integrated GO, KEGG, and network analysis and found that ribosome‐associated protein and GSTs played a vital role in the detoxification process.

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Differentially expressed genes in the fat body of Bombyx mori in response to phoxim insecticide

Cited by 31 publications

References 41 publications

Global identification of microRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

Global identification of microRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

A Comparative Studies of the Activities of Phenoloxidase, Carboxylesterase and Alkaline Phosphatase from Different Geographic Strains of Flacherie-Infected Bombyx mori

Construction and analysis of the protein–protein interaction network for the detoxification enzymes of the silkworm, Bombyx mori

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