Differentially expressed genes induced by β-caryophyllene in a rat model of cerebral ischemia-reperfusion injury

Liu, Shengwei; Liu, Jingdong; Deng, Liangliang; Chen, Sha; Wang, Xuan; Zuo, Tianrui; Hu, Qingwen; Rao, Jiangyan; Wang, Qian; Dong, Zhi

doi:10.1016/j.lfs.2021.119293

Cited by 16 publications

(14 citation statements)

References 36 publications

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“…The potential inhibitory effects of our candidate compounds as MMP‐3 or MMP‐13 inhibitors are not yet clear. However, studies suggest that they could reduce MMP‐2 and MMP‐9 levels [26–28] . Regarding the interactions of PDMs with the TNF‐α pocket, AA and BC showed the most favourable interactions, followed by α‐copaene and α‐selinene.…”

Section: Resultsmentioning

confidence: 99%

In‐Silico Selection of Wound‐Healing Plant Secondary Molecules and Their Pro‐Healing Activities on Experimental Models

Casadiego,

Macias,

García

et al. 2023

Chemistry & Biodiversity

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This study explored the potential of plant‐derived molecules (PDMs) as a medicinal treatment for skin wounds. To assess their healing properties, 34 potential drug molecules (PDMs) and ten therapeutic targets were subjected to molecular docking and dynamics analysis, with allantoin used as a standard compound. Although aristolochic acid had the most potent inhibitory effect, its toxicity made it unsuitable for testing on cells and mice. Therefore, β‐caryophyllene (BC) and caryophyllene oxide (BCoxide) were chosen for further testing. The results showed that BC‐treated HaCat cells had significantly improved scratch area closure, and both BC and BCoxide treatment produced positive effects such as reduced dermal cellularity and mast cells, decreased levels of inflammation markers IL‐6 and TNF‐α, and an increase in collagen deposition in mice tissues. However, these treatments did not accelerate wound healing. This study suggests that the PDMs selected based on in‐silico results have significant potential for pro‐healing abilities. It is essential to conduct further research to confirm our findings.

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Section: Resultsmentioning

confidence: 99%

In‐Silico Selection of Wound‐Healing Plant Secondary Molecules and Their Pro‐Healing Activities on Experimental Models

Casadiego,

Macias,

García

et al. 2023

Chemistry & Biodiversity

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“…The results obtained by Meng et al [ 36 ] showed that 817 differentially expressed mRNAs were related to the neuroprotective effect of Panax notoginseng on stroke recovery by sequencing. Liu et al [ 37 ] investigated the protective effect of β-cyanide on CIRI in rats by using mRNA sequencing and found 411 differentially expressed mRNAs—among which, the Pax1, Cxcl3 and Ccl20 genes were significantly altered. In our study, 415 mRNAs were found to be associated with the effect of 20( R )-Rg3 on stroke recovery, while the expression of these genes, such as Tagln, Vim, Ptges, Pax1, Cxcl3 and Ccl20, was also significantly altered in our sequencing results.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of the Effect of 20(R)-Ginsenoside Rg3 on Stroke Recovery in Rats via the Integrative miRNA–mRNA Regulatory Network

et al. 2022

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MicroRNAs (miRNAs) are a class of small, endogenous, noncoding RNAs. Recent research has proven that miRNAs play an essential role in the occurrence and development of ischemic stroke. Our previous studies confirmed that 20(R)-ginsenosideRg3 [20(R)-Rg3] exerts beneficial effects on cerebral ischemia–reperfusion injury (CIRI), but its molecular mechanism has not been elucidated. In this study, we used high-throughput sequencing to investigate the differentially expressed miRNA and mRNA expression profiles of 20(R)-Rg3 preconditioning to ameliorate CIRI injury in rats and to reveal its potential neuroprotective molecular mechanism. The results show that 20(R)-Rg3 alleviated neurobehavioral dysfunction in MCAO/R-treated rats. Among these mRNAs, 953 mRNAs were significantly upregulated and 2602 mRNAs were downregulated in the model group versus the sham group, whereas 437 mRNAs were significantly upregulated and 35 mRNAs were downregulated in the 20(R)-Rg3 group in contrast with those in the model group. Meanwhile, the expression profile of the miRNAs showed that a total of 283 differentially expressed miRNAs were identified, of which 142 miRNAs were significantly upregulated and 141 miRNAs were downregulated in the model group compared with the sham group, whereas 34 miRNAs were differentially expressed in the 20(R)-Rg3 treatment group compared with the model group, with 28 miRNAs being significantly upregulated and six miRNAs being significantly downregulated. Furthermore, 415 (391 upregulated and 24 downregulated) differentially expressed mRNAs and 22 (17 upregulated and 5 downregulated) differentially expressed miRNAs were identified to be related to 20(R)-Rg3′s neuroprotective effect on stroke recovery. The Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that 20(R)-Rg3 could modulate multiple signaling pathways related to these differential miRNAs, such as the cGMP-PKG, cAMP and MAPK signaling pathways. This study provides new insights into the protective mechanism of 20(R)-Rg3 against CIRI, and the mechanism may be partly associated with the regulation of brain miRNA expression and its target signaling pathways.

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“…MMPs are enzymes synthesized by fibroblasts, keratinocytes, and endothelial and inflammatory cells that normally degrade extracellular and cellsurface proteins including cytokines, chemokines, ECM proteins, cell receptors, and peptides [26]. Elevated protease activity (EPA) by MPPs of cysteine and serine proteases has been demonstrated in the tissue and fluids of human CW in contrast to acute wounds [27,28]. Some MMPs, e.g., gelatinases A and B (MMP-2 and MMP-9), collagenases 1, 2, and 3 (MMP-1, MMP-8, and MMP-13), stromelysin-1 (MMP-3), matrilysin-2 (MMP-26) and a decrease of their endogenous inhibitors (TIMP-1, and TIMP-2) was associated with non-healing CW [25].…”

Section: Molecular Dynamics Between Pdms Inhibitors and Mmp-3 Mmp-13 ...mentioning

confidence: 99%

Wound Healing Potential of Plant Secondary Metabolites: In Silico, In Vitro, and In Vivo Experiments

Casadiego¹,

Macias²,

García³

et al. 2023

Preprint

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Dysregulation of the arsenal of cells, growth factors, cytokines, proteases, and proteins involved in wound healing (WH), could delay the healing process. Plants and plant-derived molecules (PDMs) have been used as medicines for skin wounds. This study aimed to select and evaluate the pro-healing capacity of PMDs. Thirty-three PDMs and 10 therapeutic targets (TTs) were chosen for molecular docking and PDMs: aristolochic acid (AA), α-copaene, selinenes, β-caryophyllene (BC), and BCoxide, and TTs: metalloproteinase (MMP) 3, MMP13, and tumor necrosis factor (TNF α) for molecular dynamics. The bests inhibitor was AA, but because of its toxicity, BC and BCoxide were chosen for evaluation in HaCat cells and BALB/c mice excisional model. Both compounds were not toxic, and higher percentages of lesion area closure (LAC) were induced by BC treatment (p<0.05). All mice healed; however, an initial delay of LAC was induced by 0.05, 0.1% BCoxide, 0.1% BC, and allantoin. At the end of treatment, lower numbers of mast cells, level of cellular infiltrates (except 1% BCoxide and 0.05, 0.5%BC), epidermal thickness tendency (except 0.1, 0.5% BCoxide), and higher values of SC-thickness, and collagen fibers only by 0.5% BC treatment compared with untreated control (p<0.05) were observed. Our results show that BC is a promising multitarget candidate for wound healing treatment and optimization campaigns.

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Differentially expressed genes induced by β-caryophyllene in a rat model of cerebral ischemia-reperfusion injury

Cited by 16 publications

References 36 publications

In‐Silico Selection of Wound‐Healing Plant Secondary Molecules and Their Pro‐Healing Activities on Experimental Models

In‐Silico Selection of Wound‐Healing Plant Secondary Molecules and Their Pro‐Healing Activities on Experimental Models

Comprehensive Analysis of the Effect of 20(R)-Ginsenoside Rg3 on Stroke Recovery in Rats via the Integrative miRNA–mRNA Regulatory Network

Wound Healing Potential of Plant Secondary Metabolites: <em>In Silico</em>, <em>In Vitro,</em> and <em>In Vivo</em> Experiments

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