Differentially Expressed Proteins in Rat Hippocampus after Chronic Immobilization Stress and Intervention Using Xiao Yao San Decoction

Kou, Mei-Jing; Xue, Zhe; Liu, Yanyun; Liu, Yueyun; Liu, Yan; Chen, Jiaxu

doi:10.1016/s2589-3777(19)30029-1

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…Quantitative proteomics, an accurate method to reveal DEPs in TBI, can simultaneously quantify multiple proteins and predict multiple targets and drug’s mechanism of action ( Kou et al, 2018 ; Parker and Pratt, 2020 ; Suhre et al, 2021 ). Tandem mass tag-based proteomics (TMT) is a relatively quantitative proteomics technique that labels and analyzes multiple biological samples with high accuracy, sensitivity, and quality ( Hughes et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics Reveals the Dynamic Pathophysiology Across Different Stages in a Rat Model of Severe Traumatic Brain Injury

Luo

Yang

Zhang

et al. 2022

Front. Mol. Neurosci.

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BackgroundSevere traumatic brain injury (TBI) has become a global health problem and causes a vast worldwide societal burden. However, distinct mechanisms between acute and subacute stages have not been systemically revealed. The present study aimed to identify differentially expressed proteins in severe TBI from the acute to subacute phase.MethodsSixty Sprague Dawley (SD) rats were randomly divided into sham surgery and model groups. The severe TBI models were induced by the controlled cortical impact (CCI) method. We evaluated the neurological deficits through the modified neurological severity score (NSS). Meanwhile, H&E staining and immunofluorescence were performed to assess the injured brain tissues. The protein expressions of the hippocampus on the wounded side of CCI groups and the same side of Sham groups were analyzed by the tandem mass tag-based (TMT) quantitative proteomics on the third and fourteenth days. Then, using the gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), and protein–protein interaction (PPI), the shared and stage-specific differentially expressed proteins (DEPs) were screened, analyzed, and visualized. Eventually, target proteins were further verified by Western blotting (WB).ResultsIn the severe TBI, the neurological deficits always exist from the acute stage to the subacute stage, and brain parenchyma was dramatically impaired in either period. Of the significant DEPs identified, 312 were unique to the acute phase, 76 were specific to the subacute phase, and 63 were shared in both. Of the 375 DEPs between Sham-a and CCI-a, 240 and 135 proteins were up-regulated and down-regulated, respectively. Of 139 DEPs, 84 proteins were upregulated, and 55 were downregulated in the Sham-s and CCI-s. Bioinformatics analysis revealed that the differential pathophysiology across both stages. One of the most critical shared pathways is the complement and coagulation cascades. Notably, three pathways associated with gastric acid secretion, insulin secretion, and thyroid hormone synthesis were only enriched in the acute phase. Amyotrophic lateral sclerosis (ALS) was significantly enriched in the subacute stage. WB experiments confirmed the reliability of the TMT quantitative proteomics results.ConclusionOur findings highlight the same and different pathological processes in the acute and subacute phases of severe TBI at the proteomic level. The results of potential protein biomarkers might facilitate the design of novel strategies to treat TBI.

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Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics Reveals the Dynamic Pathophysiology Across Different Stages in a Rat Model of Severe Traumatic Brain Injury

Luo

Yang

Zhang

et al. 2022

Front. Mol. Neurosci.

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“…The method of Bradford was used to measure the protein levels of brain samples [19]. Bovine serum albumin (BSA; Sigma, Germany) was used as a standard.…”

Section: Determination Of Protein Levels In the Brainmentioning

confidence: 99%

Analgesic effects of Terminalia Chebula is mediated by suppression of brain protein expression of NGF and NF-κB and oxidative markers following neuropathic pain in rats

Haghani

Jaafari

Meftahi

et al. 2022

Preprint

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Background: Due to the complications related to the use of the current pharmacological approach for relief of neuropathic pain, searching for effective compound with less complication is need of present era. Since, the pathophysiology mechanism of neuropathic pain is related to excessive inflammation in nervous system. Hence, the present study focuses on whether the potential analgesic effects of Terminalia Chebula (TC) extract is mediated by the changes of brain protein expression of nerve growth factor (NGF) and nuclear factor-kappa B (NF-κB) in a rat model of sciatic nerve chronic constriction injury (CCI).Method and Results: Neuropathic pain induced by left sciatic nerve CCI. Male wistar rats assigned into sham, CCI, and CCI+TC (40mg/kg) groups. Animals received normal saline (1 ml) or aqueous-alcoholic extract of TC (40 mg/kg) for 30 days via gavage needles once a day. Cold allodynia and anxiety-like behaviors examined on one day before CCI surgery (day -1) and also on days 2, 7, 14 and 30 following CCI. We also assessed TC extract effects on oxidative stress markers on day 30 following CCI. Furthermore, western blot study performed on day 30 following CCI for evaluation of TC extract effects on protein expression of NGF and NF-κB of the brain. Orally gavage of TC extract significantly decreased cold allodynia on days 2 and 14 following CCI. Additionally, CCI model of chronic pain significantly increased protein expression of NGF and NF-κB of the brain on day 30 following CCI. Furthermore, TC extract significantly decreased protein expression of NGF and NF-κB of the brain. The TC extract significantly increased brain glutathione (GSH) content and decreased malondialdehyde (MDA) content. Conclusions: It is suggested that analgesic effects of TC extract is mediated by suppression of brain NGF, NF-κB, and also its antioxidant activity in the brain following neuropathic pain in rats.

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The therapeutic mechanism of Shenyuan Gan in lipopolysaccharide-induced neuroinflammation in BV2 microglial cells

Peng

Zhuang

et al. 2022

Digital Chinese Medicine

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Differentially Expressed Proteins in Rat Hippocampus after Chronic Immobilization Stress and Intervention Using Xiao Yao San Decoction

Cited by 5 publications

References 15 publications

Quantitative Proteomics Reveals the Dynamic Pathophysiology Across Different Stages in a Rat Model of Severe Traumatic Brain Injury

Quantitative Proteomics Reveals the Dynamic Pathophysiology Across Different Stages in a Rat Model of Severe Traumatic Brain Injury

Analgesic effects of Terminalia Chebula is mediated by suppression of brain protein expression of NGF and NF-κB and oxidative markers following neuropathic pain in rats

The therapeutic mechanism of Shenyuan Gan in lipopolysaccharide-induced neuroinflammation in BV2 microglial cells

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