Differentiating RNA from DNA by a molecular fluorescent probe based on the “door-bolt” mechanism biomaterials

Yao, Qichao; Li, Haidong; Xian, Liman; Xu, Feng; Xia, Jing; Fan, Jiangli; Du, Jianjun; Wang, Jingyun; Peng, Xiaojun

doi:10.1016/j.biomaterials.2018.05.050

Cited by 54 publications

(45 citation statements)

References 45 publications

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“…It is worth to mention that the use of excess 1‐bromo‐3‐chloropropane led to less production of the side product formed by double substitution in 1‐bromo‐3‐chloropropane . More recently, Yao et al (2018) reported the synthesis of 5 with 52.1 % yield by utilizing a methodology similar to that reported by Liu et al, 2012 …”

Section: Construction Of the Julolidine Skeletonmentioning

confidence: 90%

“…8‐Hydroxyjulolidine ( 5 ) was also used as nucleophile in reactions with diazocompound ( 155 ) (Scheme ), resulting in the salts 156a and 156b in 33.2 and 41.8 % yield, respectively . It has been shown that compound 156a can work as fluorecent probe for RNA recognition with high selectivity and rapid response, allowing for diferentiate RNA from DNA by image analysis.…”

Section: Reactions From Julolidinesmentioning

confidence: 99%

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Synthesis and Derivatization of Julolidine: A Powerful Heterocyclic Structure

Varejão

Fernandes

2019

Eur J Org Chem

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Julolidines constitute a class of N‐heterocycle compounds which have in common the 2,3,6,7‐tetrahydro‐1H,5H‐benzo[1,2]quinolizine ring. Due mainly to its fluorescence properties, such structures have shown potential application in a range of scientific and technological areas and have attracted attention of a great variety of research groups. For example, julolidines have been used for detection of ions and volatile compounds in environmental and biological samples, to the construction of dye‐sensitized solar cells, photoconductive materials and as fluorescent sensors for bioimaging. This review summarizes the strategies reported to the synthesis of the julolidine ring and the principal modifications for obtaining more complex julolidine derivatives with improved fluorescence properties of these compounds. In this context, aldol condensation, olefination, imine synthesis, and cross‐coupling reactions have been extensively explored and discussed. Examples of applications, which illustrate the great potential of such structures, will also be briefly presented.

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Section: Construction Of the Julolidine Skeletonmentioning

confidence: 90%

Section: Reactions From Julolidinesmentioning

confidence: 99%

Synthesis and Derivatization of Julolidine: A Powerful Heterocyclic Structure

Varejão

Fernandes

2019

Eur J Org Chem

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“…Besides, NBE, an NIR fluorescent probe, has no response to DNA. NBE-modified RNA probes are utilized for fulfilling excellent RNA imaging in live breast cancer and HCC cells with good photostability, high selectivity, and fast response to RNA, which facilitates the diagnosis of various cancers according to RNA contents ( Yao et al, 2018 ).…”

Section: The Application Of Nucleic Acid Probe–based Fluorescent Imaging In Cancer Diagnosis and Treatmentmentioning

confidence: 99%

“…SNPs are the most common DNA variations, and the multi-color SNP probes can discriminate four SNP variants with unique fluorescence colors, but the ideal multi-color SNP probes remain to be explored ( Obliosca et al, 2013 ). RNA probes are responsible for severe DNA interference due to the similar structures between DNA and RNA, which restrains the exploration of RNA probes ( Wang et al, 2016 ; Yao et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

Huang

Wang

et al. 2021

Front. Chem.

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It is well known that cancer incidence and death rates have been growing, but the development of cancer theranostics and therapeutics has been a challenging work. Recently, nucleic acid probe–based fluorescent sensing and imaging have achieved remarkable improvements in a variety of cancer management techniques, credited to their high sensitivity, good tolerance to interference, fast detection, and high versatility. Herein, nucleic acid probe–based fluorescent sensing and imaging are labeled with advanced fluorophores, which are essential for fast and sensitive detection of aberrant nucleic acids and other cancer-relevant molecules, consequently performing cancer early diagnosis and targeted treatment. In this review, we introduce the characteristics of nucleic acid probes, summarize the development of nucleic acid probe–based fluorescent sensing and imaging, and prominently elaborate their applications in cancer diagnosis and treatment. In discussion, some challenges and perspectives are elaborated in the field of nucleic acid probe–based fluorescent sensing and imaging.

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“…However, those fluorescent materials have some disadvantages, for instance, poor selectivity and photostability. And they always do not work in live cells 27‐29 . There are a few kinds of CDs for imaging nucleoli in live cells with excellent photostability and selectivity that have been reported in recent years 23,30 .…”

Section: Introductionmentioning

confidence: 99%

Carbonized polymer dots with dual‐wavelength emission for labeling nucleoli in live cells through hydrogen bonding‐induced aggregation

Guan

Chang

et al. 2021

Polymers for Advanced Techs

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We prepared carboxyl‐containing carbonized polymer dots (CPDs) with dual‐wavelength emission through a simple hydrothermal method. The CPDs emit blue light in live cells under the excitation at 405 nm, and show green fluorescence in cytoplasm and nucleoli when excited at 488 nm. No fluorescence can be seen in nuclei except nucleoli under the excitation at 488 nm, which means that the CPDs have excellent ability of labeling nucleoli in live cells with low background interference. This ability of the CPDs is derived from the aggregation induced by the hydrogen bonding between the carboxyl groups of the CPDs and the hydroxyl groups of ribonucleic acid at nucleoli. Fluorescence and cell culture passage experiments prove that the CPDs have no strong interaction with RNA, and all of them can be excreted out of cells in a couple of days. In vivo imaging for Caenorhabditis elegans indicates that the CPDs show the possibility of labeling nucleoli in live organisms in real‐time.

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Differentiating RNA from DNA by a molecular fluorescent probe based on the “door-bolt” mechanism biomaterials

Cited by 54 publications

References 45 publications

Synthesis and Derivatization of Julolidine: A Powerful Heterocyclic Structure

Synthesis and Derivatization of Julolidine: A Powerful Heterocyclic Structure

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

Carbonized polymer dots with dual‐wavelength emission for labeling nucleoli in live cells through hydrogen bonding‐induced aggregation

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