Differentiation in the Elymus dahuricus complex (Poaceae): evidence from grain proteins, DNA, and crossability

Abstract

The status of the taxa Elymus dahuricus Turcz. ex Griseb., E. excelsus Turcz. ex Griseb., E. woroschilowii Probat. and E. tangutorum (Nevski) Hand.-Mazz., all of which are sometimes treated as members of E. dahuricus s.l. or as constituting the E. dahuricus complex, has been investigated using morphological, biochemical (SDS-PAGE of endosperm proteins) and molecular (DNA-AFLP) variation characteristics. Populations include accessions from Siberia, Far East (Russia), Tien-Shan (Kirghizstan), and Tibet and Sichuan Provinces (China). Variation in the morphological characteristics and endosperm proteins patterns have been found in all populations of the complex. DNA variation was very low within populations but was different among populations; this was attributed to genetic drift. Seed fertility of artificial hybrids and F2-F3 progenies in 38 combinations was analyzed. All biotypes studied form a common recombination genepool (RGP). The reproductive compatibility of hybrid combinations decreases with increased geographical separation of the parents. Differences in protein patterns and in genetic variation drawn from DNA-AFLPs and phenotypical segregation in F2 on series of diagnostic characters does not support existing taxonomic treatments of the E. dahuricus complex in Southern Russia, and Central-Asian area. The E. dahuricus complex seems to be a polymorphic species having a wide geographical range and wide genetic variation. The different species need probably be relegated to infraspecific rank. We have refrained from making the necessary combinations pending examination of additional specimens, including specimens of taxa not included in this study and the type specimens of all taxa involved.

Genome constitution of Elymus tangutorum (Poaceae: Triticeae) inferred from meiotic pairing behavior and genomic in situ hybridization

“…Taxonomic treatment of E. tangutorum Elymus tangutorum differs from E. dahuricus by its spikelets disposition slightly secund, shorter glume awn, and shorter lemma awn (Baum et al, 2011). Subtle morphological differences resulted in different taxonomic treatments of the E. dahuricus complex (Peschkova, 1985(Peschkova, , 1990Probatova, 1985;Czerepanov, 1995;Agafonov et al, 2001;Baum et al, 2011 (Brysting et al, 2000). It was reported that intergenomic rearrangements could be affected by the environment (Wang et al, 2012(Wang et al, , 2014.…”

Individual variation and reproductive properties of sexual hybrids in the complex Elymus trachycaulus (Poaceae: Triticeae) and closely related taxa. Report 2. Reproductive properties of Elymus trachycaulus s. l.

“…Всего было получено 85 гибрид-ных растений F 1 в 35 комбинациях скрещивания. Все процедуры по созданию половых гибридов, оценке их семенной фертильности (СФ) и уров-ней половой совместимости биотипов Cs были выполнены, как описано ранее (Agafonov, 1997;Agafonov et al, 2001;Lu, Bothmer, 1990). Анализ репродуктивных свойств и морфологических особенностей гибридных особей в поколениях проводили на особях, выращенных на открытых делянках Центрального сибирского ботаниче-ского сада СО РАН (г. Новосибирск).…”

A taxonomic synopsis of the genus Campeiostachys Drobov

“…Cytology: 2 n = 6 x = 42 (Probatova, 1978; Probatova & Sokolovskaya, 1982). Genome StStHHYY (Agafonov et al, 2001).…”