Differentiation-Inducing Activity of Lupeol, a Lupane-Type Triterpene from Chinese Dandelion Root (Hokouei-kon), on a Mouse Melanoma Cell Line.

Hata, Keisuke; Ishikawa, Kyoko; Hori, Kazuyuki; Konishi, Tomokazu

doi:10.1248/bpb.23.962

Cited by 46 publications

(28 citation statements)

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“…The activity of B16 2F2 cells was detected widely in Compositae plants, brown algae and some mushrooms such as Tricholoma sp. (Hata et al 2000). By bioassay-guided fractionation, we isolated 4 lupane triterpenes (compounds 1-4, Fig.…”

Section: Isolation Of Melanogens From Compositae Plantsmentioning

confidence: 99%

“…By bioassay-guided fractionation, we isolated 4 lupane triterpenes (compounds 1-4, Fig. 1) as melanogens from two Compositae plants, Chinese dandelion root and Lactuca indica (Hata et al 2000;Hata et al 2003a). The cell growth and melanin contents after treatment with compound 1 (= lupeol) at various concentrations for 72 h are shown in Fig.…”

Section: Isolation Of Melanogens From Compositae Plantsmentioning

confidence: 99%

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Differentiation-inducing activity of lupane triterpenes on a mouse melanoma cell line

et al. 2006

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Lupane triterpenes were found to promote melanogenesis, a hallmark of B16 2F2 mouse melanoma cell differentiation. Studies of the structureactivity relationships demonstrated that the keto function at C-3 of the lupane skeleton played important roles in the melanogenic activities of lupane triterpenes on melanoma cells. The carbonyl group at C-17 of lupane triterpenes was essential against their apoptosis-inducing activity against human cancer cells via the inhibition of topoisomerase I. We investigated whether signaling mechanisms were involved in the stimulative effects of lupane triterpenes on the melanogenesis of B16 2F2 cells. In experiments using selective inhibitors against various signal transduction molecules and Western blotting analysis, it was suggested that p38 MAPK was involved in melanoma cell differentiation as a downstream effector of PKA. Lupeol (compound 1), a lupane triterpene, induced dendrite formations, a morphological hallmark of B16 2F2 cell differentiation by rearrangement of the actin cytoskeleton.The activation of cofilin, an actin depolymerizing factor, by compound 1 caused actin fiber disassembly in B16 2F2 cells. Furthermore, compound 1 was shown to inhibit the cell motilities of human melanoma and neuroblastoma in vitro.

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Section: Isolation Of Melanogens From Compositae Plantsmentioning

confidence: 99%

Section: Isolation Of Melanogens From Compositae Plantsmentioning

confidence: 99%

Differentiation-inducing activity of lupane triterpenes on a mouse melanoma cell line

et al. 2006

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“…Much research has concentrated on different plant extracts' abilities to induce apoptosis of cancer cells. [3][4][5] Overwhelming evidence indicates the involvement of caspases in apoptosis. Caspases make up of a family of cysteine proteases that cleave substrates after aspartic residue.…”

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confidence: 99%

Apoptosis Induced by Dioscin in Hela Cells.

Cai

Liu

Zhao

et al. 2002

Biological & Pharmaceutical Bulletin

103

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Dioscin, a saponin extracted from the root of Polygonatum Zanlanscianense Pamp, markedly inhibited proliferation of Hela cells. The results indicated that Hela cells underwent apoptosis in dose-and time-dependent manners when treated with Dioscin. Caspase-3, -8 and -9 activities were also detected. The low enzymatic activity of caspase-8 and high activity of caspase-9 showed that the mitochondrial pathway was activated in apoptosis. The reduced expression of the survival protein Bcl-2 also confirmed this result. These studies may be significant in finding a new drug to treat human cervical cancer.

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“…Several previous studies indicated that lupeol exhibits antitumor activities against melanoma cells in vitro (9,(11)(12)(13)(14)(15)(16). A previous study by Saleem et al (11) demonstrated that intraperitoneal injection of lupeol inhibits tumor growth in a human Table I.…”

Section: Discussionmentioning

confidence: 99%

Systemic and local injections of lupeol inhibit tumor growth in a melanoma-bearing mouse model

Nitta¹,

Aso²,

Hata³

et al. 2013

Biomedical Reports

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Abstract. Melanoma is the most aggressive type of skin cancer and it is procured from activated or genetically altered epidermal melanocytes. In the present study, the tumor-suppressive effects of systemic and local injections of lupeol, a triterpene extracted from Indian lettuce (Lactuca indica), in a melanoma-bearing mouse model were evaluated. Mice were injected once with lupeol or olive oil (solvent control) subcutaneously into the skin of the back or into the tumor tissue. Seven days after the injection, the tumor growth rates were calculated and the tumor tissues were collected. Immunohistochemical staining for Ki-67 and proliferating cell nuclear antigen (PCNA) were performed. The tumor growth rates in the lupeol-injected group were significantly decreased compared to those observed in the non-treated (NT) and solvent control groups. Lupeol also significantly decreased the areas positively stained for Ki-67 and PCNA in the tumor tissues compared to those in the NT and solvent control groups. The results of the present study demonstrated that systemic and local injections of lupeol suppress tumor growth and induce cell cycle arrest in a melanoma-bearing mouse model. These data suggest that lupeol may be effective as a novel therapeutic option for melanoma patients.

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Differentiation-Inducing Activity of Lupeol, a Lupane-Type Triterpene from Chinese Dandelion Root (Hokouei-kon), on a Mouse Melanoma Cell Line.

Cited by 46 publications

References 1 publication

Differentiation-inducing activity of lupane triterpenes on a mouse melanoma cell line

Differentiation-inducing activity of lupane triterpenes on a mouse melanoma cell line

Apoptosis Induced by Dioscin in Hela Cells.

Systemic and local injections of lupeol inhibit tumor growth in a melanoma-bearing mouse model

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