Differentiation of Long Non-Coding RNA and mRNA Expression Profiles in Male and Female Aedes albopictus

Xu, Ye; Dong, Yunqiao; Xu, Yang; Lai, Zetian; Jin, Baozhe; Hao, Yanqiang; Gao, Yonghui; Sun, Yan; Chen, Xiaoguang; Gu, Jiande

doi:10.3389/fgene.2019.00975

Cited by 18 publications

(10 citation statements)

References 54 publications

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“…A recent study in Ae. albopictus identified 2632 novel lncRNAs with a small fraction of these showing male- and female-specific expression patterns [ 85 ]. Work on lncRNAs in mosquitoes remains relatively novel and as a result, nothing is known about the functional consequence of genetic variation in lncRNAs.…”

Section: Long Non-coding Rnasmentioning

confidence: 99%

Filtering the Junk: Assigning Function to the Mosquito Non-Coding Genome

Farley

Eggleston

Riehle

2021

Insects

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The portion of the mosquito genome that does not code for proteins contains regulatory elements that likely underlie variation for important phenotypes including resistance and susceptibility to infection with arboviruses and Apicomplexan parasites. Filtering the non-coding genome to uncover these functional elements is an expanding area of research, though identification of non-coding regulatory elements is challenging due to the lack of an amino acid-like code for the non-coding genome and a lack of sequence conservation across species. This review focuses on three types of non-coding regulatory elements: (1) microRNAs (miRNAs), (2) long non-coding RNAs (lncRNAs), and (3) enhancers, and summarizes current advances in technical and analytical approaches for measurement of each of these elements on a genome-wide scale. The review also summarizes and highlights novel findings following application of these techniques in mosquito-borne disease research. Looking beyond the protein-coding genome is essential for understanding the complexities that underlie differential gene expression in response to arboviral or parasite infection in mosquito disease vectors. A comprehensive understanding of the regulation of gene and protein expression will inform transgenic and other vector control methods rooted in naturally segregating genetic variation.

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Section: Long Non-coding Rnasmentioning

confidence: 99%

Filtering the Junk: Assigning Function to the Mosquito Non-Coding Genome

Farley

Eggleston

Riehle

2021

Insects

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“…albopictus , knowledge on lncRNAs is still limited. Xu et al [ 30 ] reported specifically expressed lncRNAs in males and females. Azlan et al [ 29 ] have identified lncRNAs of Ae .…”

Section: Introductionmentioning

confidence: 99%

Systematic identification and characterization of long noncoding RNAs (lncRNAs) during Aedes albopictus development

et al. 2022

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Background Aedes albopictus originated in the tropical forests of Southeast Asia and can currently be found on all continents. As one of the main arboviral vectors, the control of Ae. albopictus requires novel strategies, informed by a deep knowledge of its biology. Little is known regarding mosquito long noncoding RNAs (lncRNAs), which are transcripts longer than 200 nucleotides that lack protein-coding potential and have roles in developmental regulation. Results Based on RNA-seq data from five developmental time points, eggs, early larvae, late larvae, pupae, and adults (female and male) of Ae. albopictus, 21,414 lncRNAs were characterized in this study. Differential expression analysis revealed that lncRNAs exhibited developmental stage specificity. The expression of most lncRNAs was upregulated at the onset of metamorphosis developmental stages. More differentially expressed lncRNAs were observed between eggs and early larvae. Weighted gene co-expression network analysis (WGCNA) further confirmed that the expression patterns of lncRNAs were obviously correlated with specific developmental time points. Functional annotation using co-expression analysis revealed that lncRNAs may be involved in the regulation of metamorphic developmental transitions of Ae. albopictus. The hub lncRNAs and hub gene clusters were identified for each module that were highly associated with specific developmental time points. Conclusions The results of this study will facilitate future researches to elucidate the regulatory mechanisms of lncRNAs in the development of Ae. albopictus and utilize lncRNAs to assist with mosquito control.

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“…Noncoding RNAs (ncRNA) are abundant cellular effectors of great prolific functionality ( Eddy 2001 ) and long ncRNA are defined as transcripts, more than 200 nucleotides long, that are produced by RNA polymerase II and are not translated into proteins ( Bonasio and Shiekhattar 2014 ). In Aedes lncRNAs, mainly involved in regulating gene expression, are multifunction with roles including sex differentiation ( Xu et al 2019 ), embryogenesis ( Azlan et al 2019 ), and suppression of viral replication in DENV infected mosquitoes ( Etebari et al 2016 ). Long ncRNAs have also been associated with insect’s response to xenobiotics, with reports of differential lncRNA expression in resistant populations of Plutella xylostella ( Etebari et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Expansive and Diverse Phenotypic Landscape of Field Aedes aegypti (Diptera: Culicidae) Larvae with Differential Susceptibility to Temephos: Beyond Metabolic Detoxification

Morgan

Salcedo-Sora

Triana-Chávez

et al. 2021

Journal of Medical Entomology

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Arboviruses including dengue, Zika, and chikungunya are amongst the most significant public health concerns worldwide. Arbovirus control relies on the use of insecticides to control the vector mosquito Aedes aegypti (Linnaeus), the success of which is threatened by widespread insecticide resistance. The work presented here profiled the gene expression of Ae. aegypti larvae from field populations of Ae. aegypti with differential susceptibility to temephos originating from two Colombian urban locations, Bello and Cúcuta, previously reported to have distinctive disease incidence, socioeconomics, and climate. We demonstrated that an exclusive field-to-lab (Ae. aegypti strain New Orleans) comparison generates an over estimation of differential gene expression (DGE) and that the inclusion of a geographically relevant field control yields a more discrete, and likely, more specific set of genes. The composition of the obtained DGE profiles is varied, with commonly reported resistance associated genes including detoxifying enzymes having only a small representation. We identify cuticle biosynthesis, ion exchange homeostasis, an extensive number of long noncoding RNAs, and chromatin modelling among the differentially expressed genes in field resistant Ae. aegypti larvae. It was also shown that temephos resistant larvae undertake further gene expression responses when temporarily exposed to temephos. The results from the sampling triangulation approach here contribute a discrete DGE profiling with reduced noise that permitted the observation of a greater gene diversity, increasing the number of potential targets for the control of insecticide resistant mosquitoes and widening our knowledge base on the complex phenotypic network of the Ae. aegypti response to insecticides.

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Differentiation of Long Non-Coding RNA and mRNA Expression Profiles in Male and Female Aedes albopictus

Cited by 18 publications

References 54 publications

Filtering the Junk: Assigning Function to the Mosquito Non-Coding Genome

Filtering the Junk: Assigning Function to the Mosquito Non-Coding Genome

Systematic identification and characterization of long noncoding RNAs (lncRNAs) during Aedes albopictus development

Expansive and Diverse Phenotypic Landscape of Field Aedes aegypti (Diptera: Culicidae) Larvae with Differential Susceptibility to Temephos: Beyond Metabolic Detoxification

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