Differentiation of Trichuris species eggs from non-human primates by geometric morphometric analysis

García‐Sánchez, Ángela María; Reguera-Gómez, Marta; Valero, M. Adela; Cutillas, Cristina

doi:10.1016/j.ijppaw.2020.07.001

Cited by 9 publications

(12 citation statements)

References 31 publications

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“…Recently, the morphometric approach analyzing the adult worms and eggs of Trichuris spp. of non-human primates were analyzed [33,34]. Morphometric data on the adult worms showed that features present in the females made them indistinguishable for species characteristics, but adult male worms may be used to differentiate Trichuris populations [33].…”

Section: Trichuriasis Of Manmentioning

confidence: 99%

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Trichuris spp. in Animals, with Specific Reference to Neo-Tropical Rodents

Jones

2021

Veterinary Sciences

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Trichuriasis is the clinical disease of animals infected with the parasite of the genus Trichuris. This review attempts to present information on Trichuris spp. infestation in neo-tropical rodents that are utilized for meat consumption by humans. Neo-tropical rodents utilized for meat production can be divided into two categories: those that have been domesticated, which include the guinea pig (Cavia porcellus), and those that are on the verge of domestication, such as the capybara (Hydrochoerus hydrochaeris), lappe (Cuniculus paca/Agouti paca), and agouti (Dasyprocta leporina). This document reviews the literature on the species of Trichuris that affects the rodents mentioned above, as well as the clinical signs observed. The literature obtained spans over sixty years, from 1951 to 2020. Trichuris spp. was found in these neo-tropical rodents mentioned. However, there is a dearth of information on the species of Trichuris that parasitize these animals. The capybara was the only rodent where some molecular techniques were used to identify a new species named T. cutillasae. In most cases, Trichuris spp. was found in combination with other endoparasites, and was found at a low prevalence in the lappe and guinea pig. The presence of Trichuris spp. ranged from 4.62–53.85% in the agouti, 4.21–10.00% in the lappe, 50% in the capybaras, and 1–31% in guinea pigs. Further work must be done towards molecular identification of various Trichuris spp. present in these rodents, as well as the clinical effect of infection on the performance of agouti, lappe, capybara, and guinea pigs.

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Section: Trichuriasis Of Manmentioning

confidence: 99%

“…present in non-human primates. However, further data must be collected to determine the sensitivity and specificity of this diagnostic tool [34]. Combination of various techniques, such as the use of molecular and morphological analysis, should be performed for confirmation of various Trichuris spp.…”

Section: Trichuriasis Of Manmentioning

confidence: 99%

Trichuris spp. in Animals, with Specific Reference to Neo-Tropical Rodents

Jones

2021

Veterinary Sciences

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“…In previous studies, the influence of the geographical location or host species on the size of helminth adults and eggs has been reported, for example, in species of Fasciola (Valero et al , 2001, 2002, 2009, 2012, 2018; Ashrafi et al , 2015) or Trichuris (Garcia-Sánchez et al , 2019, 2020). Nevertheless, these phenomena have so far not been described in the genus Calodium .…”

Section: Discussionmentioning

confidence: 94%

“…For this reason, new tools should be applied to advance in diagnostics. Furthermore, morphological analyses are necessary to complement molecular techniques (Valero et al , 2001, 2009, 2012; García-Sánchez et al ., 2019, 2020; Reguera-Gomez et al , 2021).…”

Section: Introductionmentioning

confidence: 99%

Variability ofCalodium hepaticumeggs from sigmodontine host species through geometric morphometric analysis

et al. 2022

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Calodium hepaticum is a zoonotic nematode with a worldwide distribution. Although the host range of C. hepaticum includes a wide spectrum of mammals (including humans), this parasite is predominantly associated with the families Muridae and Cricetidae. Several Sigmodontinae species from Argentina were found to be infected by C. hepaticum, with a high prevalence in Akodon azarae. The present study focuses on C. hepaticum eggs from natural infection of three species of sigmodontine rodents from Argentina. Eggs were genetically characterized (intergenic 18S rRNA region). The objectives of this work are: (i) to propose a new analytical methodology; and (ii) to morphologically characterize C. hepaticum eggs, from three Sigmodontinae species (A. azarae, Calomys callidus and Oligoryzomys flavescens). Analyses were made by the Computer Image Analysis System based on the new standardized measurements and geometric morphometric tools. The resulting factor maps clearly illustrate global size differences in the parasite eggs from the three Sigmodontinae species analysed. The degree of similarity between egg populations was assessed through pairwise Mahalanobis distances, showing that the largest distances were detected between parasite eggs from C. callidus and O. flavescens. Herein, the phenotypical plasticity of C. hepaticum eggs is shown. Significant positive correlations were obtained between each egg parasite principal component 1 and rodent corporal characteristics: weight; liver weight; rodent length; and rodent body condition. The usefulness of the geometric morphometric analysis in studies of the relationship between C. hepaticum and its host must be highlighted. The high prevalence observed in A. azarae, associated with the wide size range of the parasite eggs evidenced by principal component analysis, suggests A. azarae to be the Sigmodontinae host species that plays the most important role as reservoir host for C. hepaticum in the New World.

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“…Primate stool samples were collected, smeared onto glass slides, covered with coverslips, and recorded using photographs taken under a light microscope. The pictures were uploaded into a computer program, scaled, and digitized, and the shape patterns were statistically analyzed using principal component analysis, canonical variate analysis, and Mahalanobis distance [ 65 ]. In addition, Hugot and Baylac (2007) identified pinworms (Enterobiinae) using a landmark analysis [ 47 ].…”

Section: Advanced Approaches For Medical Parasite and Arthropod Diagnosesmentioning

confidence: 99%

State-of-the-Art Techniques for Diagnosis of Medical Parasites and Arthropods

Ruenchit

2021

Diagnostics

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Conventional methods such as microscopy have been used to diagnose parasitic diseases and medical conditions related to arthropods for many years. Some techniques are considered gold standard methods. However, their limited sensitivity, specificity, and accuracy, and the need for costly reagents and high-skilled technicians are critical problems. New tools are therefore continually being developed to reduce pitfalls. Recently, three state-of-the-art techniques have emerged: DNA barcoding, geometric morphometrics, and artificial intelligence. Here, data related to the three approaches are reviewed. DNA barcoding involves an analysis of a barcode sequence. It was used to diagnose medical parasites and arthropods with 95.0% accuracy. However, this technique still requires costly reagents and equipment. Geometric morphometric analysis is the statistical analysis of the patterns of shape change of an anatomical structure. Its accuracy is approximately 94.0–100.0%, and unlike DNA barcoding, costly reagents and equipment are not required. Artificial intelligence technology involves the analysis of pictures using well-trained algorithms. It showed 98.8–99.0% precision. All three approaches use computer programs instead of human interpretation. They also have the potential to be high-throughput technologies since many samples can be analyzed at once. However, the limitation of using these techniques in real settings is species coverage.

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Differentiation of Trichuris species eggs from non-human primates by geometric morphometric analysis

Cited by 9 publications

References 31 publications

Trichuris spp. in Animals, with Specific Reference to Neo-Tropical Rodents

Trichuris spp. in Animals, with Specific Reference to Neo-Tropical Rodents

Variability ofCalodium hepaticumeggs from sigmodontine host species through geometric morphometric analysis

State-of-the-Art Techniques for Diagnosis of Medical Parasites and Arthropods

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