Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model: A Proof of Concept

Restrepo, Lina; Murillo, Javier; Botina, Deivid; Zarzycki, Artur; Garzón, Johnson; Franco, Ricardo; Montaño, Jaime; Calderon, Samuel; Torres-Madronero, Maria C.; Marzani, Franck; Robledo, Sara M.; Galeano, July

doi:10.1177/24726303211030292

Cited by 1 publication

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“…Color and multispectral imaging can be used for wound healing evaluation [16,17]. For the estimation of biological variables in the tissues, diffuse reflectance models are used which allow the quantification of the biological components of the skin through a spectral signature [18,19]. Spectral analysis is widely used to analyze cutaneous pathologies by quantifying parameters such as epidermis thickness, keratinocytes, collagen, oxygen saturation (SpO 2 ), etc.…”

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confidence: 99%

Temporal Evaluation of the Surface Area of Treated Skin Ulcers Caused by Cutaneous Leishmaniasis and Relation with Optical Parameters in an Animal Model: A Proof of Concept

Londoño

Viloria

Pérez-Buitrago

et al. 2023

Sensors

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Cutaneous leishmaniasis (CL) is a neglected disease caused by an intracellular parasite of the Leishmania genus. CL lacks tools that allow its understanding and treatment follow-up. This article presents the use of metrical and optical tools for the analysis of the temporal evolution of treated skin ulcers caused by CL in an animal model. Leishmania braziliensis and L. panamensis were experimentally inoculated in golden hamsters, which were treated with experimental and commercial drugs. The temporal evolution was monitored by means of ulcers’ surface areas, as well as absorption and scattering optical parameters. Ulcers’ surface areas were obtained via photogrammetry, which is a procedure that allowed for 3D modeling of the ulcer using specialized software. Optical parameters were obtained from a spectroscopy study, representing the cutaneous tissue’s biological components. A one-way ANOVA analysis was conducted to identify relationships between both the ulcers’ areas and optical parameters. As a result, ulcers’ surface areas were found to be related to the following optical parameters: epidermis thickness, collagen, keratinocytes, volume-fraction of blood, and oxygen saturation. This study is a proof of concept that shows that optical parameters could be associated with metrical ones, giving a more reliable concept during the assessment of a skin ulcer’s healing.

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