Diffusion and imaging properties of three new lipophilic tracers, NeuroVue™ Maroon, NeuroVue™ Red and NeuroVue™ Green and their use for double and triple labeling of neuronal profile

Fritzsch, Bernd; Muirhead, Katharine A.; Feng, Feng; Gray, Brian; Ohlsson‐Wilhelm, Betsy M.

doi:10.1016/j.brainresbull.2005.05.016

Cited by 64 publications

(80 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6G,7E,F), many of the proximal segments of the axons of cells that exit the olfactory bulb are heavily myelinated. The lipophilic dye DiI is differentially taken up by lipid-rich myelin sheaths, so that little or no dye remains to diffuse within the axon proper (Fritzsch et al, 2005). This situation is further confounded by the secondary diffusion of the DiI from the myelin sheaths into what appear to be the apical processes of ependymal cells, which elevates the general background of the tissue, confounding the detection of fine fibers and their terminals.…”

Section: Secondary Olfactory Projectionsmentioning

confidence: 93%

Olfactory projections in the white sturgeon, Acipenser transmontanus: An experimental study

Northcutt

2011

J of Comparative Neurology

View full text Add to dashboard Cite

Telencephalic evolution in ray-finned fishes shows increasing complexity from polypteriform fishes through sturgeons to teleosts. Telencephalic organization in sturgeons is thus critical to our understanding of ray-finned fish evolution, but it is poorly understood, particularly as regards the roof or pallium. Two major hypotheses exist regarding the medial part of area dorsalis (Dm): that Dm is extended; and that Dm is restricted. The extent and topography of secondary olfactory projections to the pallium are critical in evaluating these hypotheses, but there is little agreement regarding these projections. Olfactory projections in the white sturgeon were therefore examined by using the carbocyanine probe DiI, biocytin, and biotinylated dextrin amine (BDA). Both DiI and BDA revealed primary olfactory projections to the olfactory bulb and primary extrabulbar projections widely in the telencephalon and to more restricted regions of the diencephalon. Myelinated secondary olfactory fibers caused DiI to be less effective in labeling secondary olfactory projections, which terminate in all subpallial nuclei and in the pallium: sparsely in the medial pallial division (Dm); heavily in the posterior pallial division (Dp); and more lightly in the lateral pallial division (Dl). In the diencephalon, substantial secondary olfactory projections were seen to the habenular nuclei, the rostral pole of the inferior lobe, and several nuclei of the posterior tubercle. All secondary olfactory projections were bilateral but heavier ipsilaterally. Bulbopetal neurons were located in both pallial and subpallial centers and were more numerous ipsilaterally. These results corroborate an earlier experimental study on the shovelnose sturgeon and indicate a restricted Dm in sturgeons.

show abstract

Section: Secondary Olfactory Projectionsmentioning

confidence: 93%

Olfactory projections in the white sturgeon, Acipenser transmontanus: An experimental study

Northcutt

2011

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…FBMs were labeled with lipophilic dyes (NeuroVue; Molecular Targeting Technologies; MTTI) (Fritzsch, et al, 2005). The E12.5 and the E13.5 brains were labeled from both sides.…”

Section: Methodsmentioning

confidence: 99%

“…This dye was false colored as green during imaging. Another dye labelling with a different color was placed into the right ear to label the vestibulo-cochlear efferent and facial motor neurons on the right side, which was false colored as red (Fritzsch, et al, 2005, Maklad and Fritzsch, 2003). The E12.5 and the E13.5 hindbrain was incubated in 4% PFA at 60°C for two days, dissected out, and mounted in open-book configuration in glycerol.…”

Section: Methodsmentioning

confidence: 99%

Prickle1 is necessary for the caudal migration of murine facial branchiomotor neurons

et al. 2014

View full text Add to dashboard Cite

Facial branchiomotor neurons (FBMs) of vertebrates typically develop in rhombomere 4 (r4) and in mammals and several other vertebrate taxa, migrate caudally into r6 and subsequently laterally and ventrally to the pial surface. How similar or dissimilar these migratory processes between species are at a molecular level remains unclear. In zebrafish and mouse, mutations in certain PCP genes disrupt normal caudal migration of FBMs. Zebrafish prickle1a (prickle-like 1a) and prickle1b, two orthologs of Prickle1, act non-cell autonomously and cell-autonomously, respectively, to regulate FBM migration. Here we show that in Prickle1C251X/C251X mice, which have reduced Prickle1 expression, the caudal migration of FBMs is affected. Most FBM neurons do not migrate caudally along the floor plate. However, some neurons perform limited caudal migration such that the neurons eventually lie near the pial surface from r4 to anterior r6. FBMs in Prickle1C251X/C251X mice survive until P0 and form an ectopic nucleus dorsal to the olivo-cochlear efferents of r4. Ror2, which modifies the PCP pathway in other systems, is expressed by the migrating mouse FBMs, but is not required for FBM caudal migration. Our results suggest that in mice, Prickle1 is part of a molecular mechanism that regulates FBM caudal migration and separates the FBM and the olivo-cochlear efferents. This defective caudal migration of FBMs in Prickle1C251X mutants resembles Vangl2 mutant defects. In contrast to other developing systems that show similar defects in Prickle1, Wnt5a and Ror2, Wnt5a and Ror2 only have limited or no effect on FBM caudal migration.

show abstract

“…Lipophilic dye-soaked filter paper (Fritzsch et al, 2005) was used to backfill from the spinal cord to the transplanted ear. Embryos were first fixed in 10% PFA following anesthesia.…”

Section: Lipophilic Dye Labelmentioning

confidence: 99%

Transplantation of Xenopus laevis ears reveals the ability to form afferent and efferent connections with the spinal cord

Elliott

Fritzsch²

2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Diffusion and imaging properties of three new lipophilic tracers, NeuroVue™ Maroon, NeuroVue™ Red and NeuroVue™ Green and their use for double and triple labeling of neuronal profile

Cited by 64 publications

References 29 publications

Olfactory projections in the white sturgeon, Acipenser transmontanus: An experimental study

Olfactory projections in the white sturgeon, Acipenser transmontanus: An experimental study

Prickle1 is necessary for the caudal migration of murine facial branchiomotor neurons

Transplantation of Xenopus laevis ears reveals the ability to form afferent and efferent connections with the spinal cord

Contact Info

Product

Resources

About