Diffusion coefficient of alginate microcapsules used in pancreatic islet transplantation, a method to cure type 1 diabetes

Najdahmadi, Avid; Lakey, Jonathan R. T.; Botvinick, Elliot

doi:10.1117/12.2318565

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…For the CMC capsule conditions, the saturation in the shell and core reach a common plateau of fluorescence of approximately 60% after 1 h and stay at similar intensity values until 3 h. For the XG capsules, although the shell reaches a plateau of above 70%, the diffusion to the core flattens at 60 min around 50% and does not further increase but sinks below 40% until hour 3. Generally, diffusion below 40–70 kDa has been shown previously for alginate-based solid beads [ 34 , 35 , 36 ], but a slightly better diffusion into the core is seen in capsules with CMC used as a thickening agent in this approach.…”

Section: Resultsmentioning

confidence: 54%

Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells

et al. 2022

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Mesenchymal stem cells (MSCs) are primary candidates in tissue engineering and stem cell therapies due to their intriguing regenerative and immunomodulatory potential. Their ability to self-assemble into three-dimensional (3D) aggregates further improves some of their therapeutic properties, e.g., differentiation potential, secretion of cytokines, and homing capacity after administration. However, high hydrodynamic shear forces and the resulting mechanical stresses within commercially available dynamic cultivation systems can decrease their regenerative properties. Cells embedded within a polymer matrix, however, lack cell-to-cell interactions found in their physiological environment. Here, we present a “semi scaffold-free” approach to protect the cells from high shear forces by a physical barrier, but still allow formation of a 3D structure with in vivo-like cell-to-cell contacts. We highlight a relatively simple method to create core–shell capsules by inverse gelation. The capsules consist of an outer barrier made from sodium alginate, which allows for nutrient and waste diffusion and an inner compartment for direct cell-cell interactions. Next to capsule characterization, a harvesting procedure was established and viability and proliferation of human adipose-derived MSCs were investigated. In the future, this encapsulation and cultivation technique might be used for MSC-expansion in scalable dynamic bioreactor systems, facilitating downstream procedures, such as cell harvest and differentiation into mature tissue grafts.

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Section: Resultsmentioning

confidence: 54%

Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells

et al. 2022

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Modeling and optimization of the niosome nanovesicles using response surface methodology for delivery of insulin

Hakamivala

Moghassemi

Omidfar

2019

Biomed. Phys. Eng. Express

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Niosome is a drug carrier with high stability and biocompatibility that provides higher drug availability, which results in improved therapeutic performance. Despite some promises that show the potential of using niosome for oral delivery of insulin, the effect of multiple factors such as the concentration of the ingredients, mechanical forces, and experimental conditions on final products make the synthesis of insulin-loaded niosomal particle with the proper characteristics very challenging. In this study, the effects of different factors including the concentration of cholesterol and surfactant, as well as duration of sonication, on zeta-potential, polydispersity index (PDI), and entrapment efficiency (EE%) of the insulin-loaded niosomal vesicles were evaluated and optimum condition was assessed in terms of cytocompatibility and in vitro drug release. The results show that the cholesterol concentration and sonication time have a significant influence on the zeta-potential, whereas the surfactant concentration plays no role. In addition, PDI increased with an increase in cholesterol and surfactant concentration but decreased with increase in the duration of sonication. Furthermore, increasing cholesterol concentration increased EE and increasing the duration of sonication decreased EE. Considering the effect of different factors, the optimum condition, which is defined as minimum PDI, maximum EE, and zeta potential >±30 mV, occurred while the concentration of the cholesterol and surfactant was 1 M and sonication time was 10 min. The zeta potential, PDI, and EE of the optimum insulin-loaded niosomal vesicles were 36.23±1.04 mV, 0.53±0.07 and 79±2%, which were very close to the predicted values, with the prediction error below 5%. Finally, the optimum insulin-loaded niosomal vesicles were characterized in different aspects. They displayed a spherical morphology with a low tendency to be aggregated. In addition, they showed good biocompatibility with Caco-2 cells and slow release in simulated intestinal fluid.

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Diffusion coefficient of alginate microcapsules used in pancreatic islet transplantation, a method to cure type 1 diabetes

Cited by 2 publications

References 38 publications

Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells

Alginate Core–Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells

Modeling and optimization of the niosome nanovesicles using response surface methodology for delivery of insulin

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