Diffusion coefficient of cationic liposomes during lipoplex formation determines transfection efficiency in HepG2 cells

Hu, Die; Fumoto, Shintaro; Yoshikawa, Naoki; Peng, Jianqing; Miyamoto, Hirotaka; Tanaka, Masakazu; Nishida, Koyo

doi:10.1016/j.ijpharm.2023.122881

Cited by 6 publications

(8 citation statements)

References 45 publications

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“…The process achieved a particle size of around 138 nm with a narrow size distribution for particular N/P formulation. Similar results of 131 nm were obtained with cationic lipid particles through thin film hydration method . The lipid composition also plays a key role in the particle size.…”

Section: Discussionmentioning

confidence: 94%

“…Similar results of 131 nm were obtained with cationic lipid particles through thin film hydration method. 5 The lipid composition also plays a key role in the particle size. The delivery vehicle performs to its optimum level owing to its composition, charge, and size.…”

Section: Discussionmentioning

confidence: 99%

“…Cationic liposomes were employed to deliver plasmid DNA where the particle size dependent endocytosis was observed. 5 Polyethylenimine, both branched and unbranched polymers, was a popular choice of plasmid DNA nonviral gene delivery vehicles. The cationic polymer was used for delivery of fibroblast growth factor (pFGF), 6 bone morphogenic protein (pBMP2), 7 vascular endothelial growth factor (pVEGF), 8 and plasma-derived growth factor (pPDGF) 9 genes in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

“…Nanoparticle delivery vehicles such as liposomes, polyethylenimine/cationic polymers are widely employed to create nonviral gene delivery strategies. Cationic liposomes were employed to deliver plasmid DNA where the particle size dependent endocytosis was observed . Polyethylenimine, both branched and unbranched polymers, was a popular choice of plasmid DNA nonviral gene delivery vehicles.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Proof-of-Concept Preparation of Lipid–Plasmid DNA Particles Using Novel Extrusion-Based 3D-Printing Technology, SMART

Chakka,

Maniruzzaman

2023

Mol. Pharmaceutics

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Gene therapy is a promising approach with delivery of mRNA, small interference RNA, and plasmid DNA to elicit a therapeutic action in vitro using cationic or ionizable lipid nanoparticles. In the present study, a novel extrusion-based Sprayed Multi Adsorbed-droplet Reposing Technology (SMART) developed in-house was employed for the preparation, characterization, and transfection abilities of the green fluorescence protein (GFP) plasmid DNA in cancer cells in vitro. The results showed 100% encapsulation of pDNA (GFP) in LNPs of around 150 nm (N/P 5) indicating that the processes developed using SMART technology are consistent and can be utilized for commercial applications.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Proof-of-Concept Preparation of Lipid–Plasmid DNA Particles Using Novel Extrusion-Based 3D-Printing Technology, SMART

Chakka,

Maniruzzaman

2023

Mol. Pharmaceutics

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“…Plasmid DNA accessibility in the ternary complexes was decreased by incubation with PBS, whereas incubation with serum, heat-inactivated serum, or BSA in PBS did not significantly change the accessibility ( Figure 8 ). Recently, we found that plasmid DNA accessibility was positively correlated with the transfection efficiency of different cationic liposome/plasmid DNA complexes prepared under various conditions, in terms of liposomal size and temperature [ 48 ]. Therefore, there was a discrepancy between the ternary complexes and cationic liposome/plasmid DNA complexes in terms of the relationship between plasmid DNA accessibility and transfection efficiency.…”

Section: Discussionmentioning

confidence: 99%

Interaction of γ-Polyglutamic Acid/Polyethyleneimine/Plasmid DNA Ternary Complexes with Serum Components Plays a Crucial Role in Transfection in Mice

Ko,

Fumoto,

Kurosaki

et al. 2024

Pharmaceutics

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Typical examples of non-viral vectors are binary complexes of plasmid DNA with cationic polymers such as polyethyleneimine (PEI). However, problems such as cytotoxicity and hemagglutination, owing to their positively charged surfaces, hinder their in vivo use. Coating binary complexes with anionic polymers, such as γ-polyglutamic acid (γ-PGA), can prevent cytotoxicity and hemagglutination. However, the role of interactions between these complexes and serum components in in vivo gene transfer remains unclear. In this study, we analyzed the contribution of serum components to in vivo gene transfer using PEI/plasmid DNA binary complexes and γ-PGA/PEI/plasmid DNA ternary complexes. In binary complexes, heat-labile components in the serum greatly contribute to the hepatic and splenic gene expression of the luciferase gene. In contrast, serum albumin and salts affected the hepatic and splenic gene expression in the ternary complexes. Changes in physicochemical characteristics, such as increased particle size and decreased absolute values of ζ-potential, might be involved in the enhanced gene expression. These findings would contribute to a better understanding of in vivo non-viral gene transfer using polymers, such as PEI and γ-PGA.

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Molecular Dynamics Simulations Reveal Octanoylated Hyaluronic Acid Enhances Liposome Stability, Stealth and Targeting

Zhu,

Xu,

Wang

et al. 2024

ACS Omega

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Liposome-based drug delivery systems have been widely used in drug and gene delivery. However, issues such as instability, immune clearance, and poor targeting have significantly limited their clinical utility. Consequently, there is an urgent need for innovative strategies to improve liposome performance. In this study, we explore the interaction mechanisms of hyaluronic acid (HA), a linear anionic polysaccharide composed of repeating disaccharide units of D-glucuronic acid and N-acetyl-D-glucosamine connected by alternating β-1,3 and β-1,4 glycosidic linkages, and its octanoylated derivates (OHA) with liposomes using extensive coarse-grained molecular dynamics simulations. The octyl moieties of OHA spontaneously inserted into the phospholipid bilayer of liposomes, leading to their effective coating onto the surface of liposome and enhancing their structural stability. Furthermore, encapsulating liposome with OHA neutralized their surface potential, interfering with the formation of a protein corona known to contribute to liposomal immune clearance. Importantly, the encapsulated OHA maintained its selectivity and therefore targeting ability for CD44, which is often overexpressed in tumor cells. These molecular-scale findings shed light on the interaction mechanisms between HA and liposomes and will be useful for the development of next-generation liposome-based drug delivery systems.

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Diffusion coefficient of cationic liposomes during lipoplex formation determines transfection efficiency in HepG2 cells

Cited by 6 publications

References 45 publications

A Proof-of-Concept Preparation of Lipid–Plasmid DNA Particles Using Novel Extrusion-Based 3D-Printing Technology, SMART

A Proof-of-Concept Preparation of Lipid–Plasmid DNA Particles Using Novel Extrusion-Based 3D-Printing Technology, SMART

Interaction of γ-Polyglutamic Acid/Polyethyleneimine/Plasmid DNA Ternary Complexes with Serum Components Plays a Crucial Role in Transfection in Mice

Molecular Dynamics Simulations Reveal Octanoylated Hyaluronic Acid Enhances Liposome Stability, Stealth and Targeting

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