Diffusion of Oligonucleotides from within Iron‐Cross‐Linked, Polyelectrolyte‐Modified Alginate Beads: A Model System for Drug Release

Abstract

ABTRACT:We developed and experimentally verified an analytical model to describe diffusion of oligonucleotides from stable hydrogel beads. The synthesized alginate beads are

Glucose‐Triggered Insulin Release from Fe3+‐Cross‐linked Alginate Hydrogel: Experimental Study and Theoretical Modeling

“…The enzyme GOx was attached to the SiO 2 ‐NPs to prevent its leakage from the alginate particles. The particles were additionally stabilized with PAH homogeneously distributed in the particle volume in order to minimize the uncontrolled leakage of insulin . In addition, a thin layer of PAH was deposited on the surface of the particles for their further stabilization and to decrease the uncontrolled leakage of insulin.…”

“…The particles were additionally stabilized with PAHh omogeneously distributed in the particle volumei no rder to minimize the uncontrolled leakage of insulin. [48] In addition, athin layer of PAHw as deposited on the surface of the particlesf or their further stabilization and to decrease the uncontrolledl eakage of insulin.I ts hould be noted that some other positively charged polymers,e .g.,p olylysine [55] can be also used for stabilizing alginateh ydrogels and protecting entrapped biomolecules from leakage. However,e xperimentally PAHw as shown to be the most effective.…”

“…Our experimental study of the release system has provided data for theoretical modeling that offers additional understanding the system's performance and allows prediction of additional features of the realized system. The new results reported here allow modeling of the signal‐triggered release process beyond the earlier reported study of the diffusional leakage of biomolecules from alginate beads . Systems of higher complexity, involving multi‐signal triggering of the release process are under investigation.…”

“…The new results reportedh ere allow modeling of the signal-triggered release process beyond the earlier reported study of the diffusional leakage of biomolecules from alginate beads. [48] Systemso f higher complexity,i nvolving multi-signalt riggering of the releaseprocess are under investigation. Fluorescent measurements were performed using af luorescent spectrophotometer (Varian, Cary Eclipse).…”

DNA Release from Fe3+‐Cross‐Linked Alginate Films Triggered by Logically Processed Biomolecular Signals: Integration of Biomolecular Computing and Actuation

“…In this experiment, the alginate hydrogel with the entrapped DNA‐FAM was produced in the form of small beads (ca. 1 mm diameter), following the experimental procedure used in our previously published report . The alginate beads were used in this experiment instead of a film on the electrode surface because the Ca 2+ ‐cross‐linked alginate cannot be deposited on an electrode surface electrochemically.…”