Diffusion quasiélastique de la lumière sous champ électrique : mobilité électrophorétique et charge apparente des macromolécules

Drifford, M.; Menez, Raphael; Tivant, P.; Nectoux, P.; Dalbiez, Jean‐Pierre

doi:10.1051/rphysap:0198100160201900

Cited by 16 publications

(8 citation statements)

References 28 publications

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“…Furthermore, except at extremely low I, literature results for the mobility of poly(vinyl sulfate) 37 and poly-(acrylic acid) 40 can be neatly overlapped onto the data of Figure 12 after suitable correction for temperature differences between experiments. To be totally objective about this overlap, we must note that other literature data sets 26,28,42 do not superimpose so well in this I regime. However, these poorly superimposing data mostly come from studies characterized by large experimental error.…”

Section: Resultsmentioning

confidence: 99%

“…The first relevant theory 16 and systematic experimental investigation 17 of polyelectrolyte electrophoresis appeared in the 1930s. Subsequent investigations of free solution electrophoresis have focused mostly on biopolymers, 3,7,8,13,[18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] but a significant number of synthetic polymers have also been examined. 1,28,[35][36][37][38][39][40][41][42][43][44][45][46][47] Figure 1, compiling data from eight independent sources, 19,20,[23][24][25]27,28,34 shows µ 0 as a function of I for high molecular weight duplex DNA in aqueous NaCl; this system has been studied most often.…”

Section: Introductionmentioning

confidence: 99%

“…Subsequent investigations of free solution electrophoresis have focused mostly on biopolymers, 3,7,8,13,[18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] but a significant number of synthetic polymers have also been examined. 1,28,[35][36][37][38][39][40][41][42][43][44][45][46][47] Figure 1, compiling data from eight independent sources, 19,20,[23][24][25]27,28,34 shows µ 0 as a function of I for high molecular weight duplex DNA in aqueous NaCl; this system has been studied most often. The large disparities in the data sets of Figure 1 plainly reveal why theoretical models for µ 0 have been so difficult to evaluate.…”

Section: Introductionmentioning

confidence: 99%

“…At high I, counterions cluster tightly to the chain and F c is large, while at low I, counterions cluster less tightly and F c is small. At the limit of zero 19,20,[23][24][25]27,28,34 The preceding arguments become invalid if the applied field, the local solvent flow, or the electromigration of counterions modifies the equilibrium structures of the chain or its ion cloud. Such modifications introduce nonlinearities into the analysis of solute motion.…”

Section: Introductionmentioning

confidence: 99%

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Capillary Electrophoresis Measurements of the Free Solution Mobility for Several Model Polyelectrolyte Systems

1999

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The free solution electrophoretic mobilities of poly(styrenesulfonate), ss-DNA, and duplex DNA are measured by capillary electrophoresis across a range of ionic strengths and, for poly(styrenesulfonate) and ss-DNA, across a range of chain lengths. The data are then compared with mobilities reported in the literature and predicted by theory. For ionic strengths below 0.1 M, the capillary method is more accurate and rapid than previous techniques; it also provides a distribution of mobility values for polyelectrolyte mixtures. A maximum of the free solution mobility with respect to chain length is discovered in the oligomer range for both poly(styrenesulfonate) and ss-DNA; lowering ionic strength accentuates this unexplained phenomenon. In the large chain limit, where the mobility is independent of chain length, the ionic strength dependences of mobility for all three polymers are remarkably similar. These dependences can only be explained by models that incorporate nonlinear electrostatic effects into the description of the counterion cloud. The Manning model (with relaxation correction) best approximates the dependence of mobility on ionic strength.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Capillary Electrophoresis Measurements of the Free Solution Mobility for Several Model Polyelectrolyte Systems

1999

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“…Cette technique, qui nécessite l'emploi de sources monochromatiques très intenses (donc de lasers), fut employée pour la première fois par Yeh et Cummins en 1964 [13]. La vélocimétrie Dopplerlaser, dont il existe différentes variantes [14], a été utilisée sous champ électrique en veine liquide initialement par Ware et Flygare [15,16], et Uzgiris [17,18] ; depuis de nombreux groupes ont développé et adapté cette méthode en fonction des différents types d'échantillons étudiés [19][20][21][22][23][24][25][26][27]. Une mise au point sur la méthode et ses applications a été publiée par Uzgiris [28,29], et Ware et Haas [30].…”

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Dispositif de microélectrophorèse utilisant un vélocimètre optique original à réseau de transmission : aspects théoriques et méthodologiques

Delatour-Hanss

1989

Rev. Phys. Appl. (Paris)

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Cyclic migration of DNA in gels: DNA stretching and electrophoretic mobility

Åkerman¹

1996

Electrophoresis

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Available data from spectroscopic and microscopy studies of electrophoretic orientation of long DNA (above 40 kbp) in agarose gels is analyzed on the basis of the fact that the migration in constant fields is cyclic in nature. Defining a cycle period as the time between two consecutive compact states, a simple model is used to obtain data on the average time period (< T >) and the step length (< L >) of the migration cycle from spectroscopic measurements of the dynamics of helix orientation and center-of-mass velocity. Furthermore, the degree of orientation is used to analyze tube-orientation and DNA stretching contributions to < L > and < T >. Finally, the average electrophoretic velocity v = < L >/< T > is analyzed in terms of < L > and < T > for different DNA sizes (Lc), field strengths (E), and gel concentrations (A). The main results of the analysis are: (i) the increase and saturation of the electrophoretic mobility with increasing E is mainly governed by < L > via the degree of DNA stretching, (ii) DNA molecules of different sizes migrate with the same velocity because < L > and < T > both increase approximately linearly with Lc, and (iii) migration in a denser gel is slower mainly because < T > increases, while the step length is approximately constant. Assuming the charge Q of DNA is the same as in free solution, these results suggest that the reason the fundamental reptation equation for the electrophoretic mobility mu = (Q/zeta) < (hx/Lt)2 > also applies in the presence of strong fluctuations in the tube length Lt, and end-to-end distance hx, is that the friction coefficient zeta for motion along the tube is lower the more stretched the DNA is.

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Diffusion quasiélastique de la lumière sous champ électrique : mobilité électrophorétique et charge apparente des macromolécules

Cited by 16 publications

References 28 publications

Capillary Electrophoresis Measurements of the Free Solution Mobility for Several Model Polyelectrolyte Systems

Capillary Electrophoresis Measurements of the Free Solution Mobility for Several Model Polyelectrolyte Systems

Dispositif de microélectrophorèse utilisant un vélocimètre optique original à réseau de transmission : aspects théoriques et méthodologiques

Cyclic migration of DNA in gels: DNA stretching and electrophoretic mobility

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