Diffusion Theory for the Infection Pathway of Virus in a Living Cell

Itto, Yuichi

doi:10.2174/2210289201809010001

“…Note added. In a recent paper [28], an issue regarding the continuum limit of the entropy [14][15][16] has been carefully examined. The discussion given there is seen to be applicable to the entropy in Eq.…”

Section: Acknowledgementsmentioning

confidence: 99%

Gaussian fluctuation of the diffusion exponent of virus capsid in a living cell nucleus

Itto

¹

2018

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In their work [Proc. Natl. Acad. Sci. USA 112 (2015) E5725], Bosse et al. experimentally showed that virus capsid exhibits not only normal diffusion but also anomalous diffusion in nucleus of a living cell. There, it was found that the distribution of fluctuations of the diffusion exponent characterizing them takes the Gaussian form, which is, quite remarkably, the same form for two different types of the virus. This suggests high robustness of such fluctuations. Here, the statistical property of local fluctuations of the diffusion exponent of the virus capsid in the nucleus is studied. A maximum-entropy-principle approach (originally proposed for a different virus in a different cell) is applied for obtaining the fluctuation distribution of the exponent.Largeness of the number of blocks identified with local areas of interchromatin corrals is also examined based on the experimental data. It is shown that the Gaussian distribution of the local fluctuations can be derived, in accordance with the above form.In addition, it is quantified how the fluctuation distribution on a long time scale is different from the Gaussian distribution.

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“…Interestingly, the recorded intracellular trajectories of ND-CPs are in line with previous reports on the diffusion of adeno-associated virions in the cytoplasm of a living HeLa cell, where intracellular trajectories have also been classified as anomalous diffusion and normal diffusion. 54 It has been speculated that anomalous diffusion occurred due to nonspecific interactions between the virions and obstacles inside the cytoplasm such as organelles, which could also be the origin of the anomalous diffusion of ND-CPs. Therefore, ND-CPs consisting of a fluorescent nanodiamond core and capsid protein corona could represent valuable tools to elucidate the infection pathway of the virions over the cytoplasm.…”

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confidence: 99%

Fluorescent Nanodiamonds Encapsulated by Cowpea Chlorotic Mottle Virus (CCMV) Proteins for Intracellular 3D-Trajectory Analysis

Wu¹,

Cao²,

Alam³

et al. 2021

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0

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Long-term tracking of nanoparticles to resolve intracellular structures and motions is essential to elucidate fundamental parameters as well as transport processes within living cells. Fluorescent nanodiamond (ND) emitters provide cell compatibility and very high photostability. However, high stability, biocompatibility, and cellular uptake of these fluorescent NDs under physiological conditions are required for intracellular applications. Herein, highly stable NDs encapsulated with Cowpea chlorotic mottle virus capsid proteins (ND-CP) are prepared. A thin capsid protein layer is obtained around the NDs, which imparts reactive groups and high colloidal stability, while retaining the opto-magnetic properties of the coated NDs as well as the secondary structure of CPs adsorbed on the surface of NDs. In addition, the ND-CP shows excellent biocompatibility both in vitro and in vivo. Long-term 3D trajectories of the ND-CP with fine spatiotemporal resolutions are recorded; their intracellular motions are analyzed by different models, and the diffusion coefficient are calculated. The ND-CP with its brilliant optical properties and stability under physiological conditions provides us with a new tool to advance the understanding of cell biology, e.g., endocytosis, exocytosis, and active transport processes in living cells as well as intracellular dynamic parameters. <br>

show abstract

“…Interestingly, the recorded intracellular trajectories of ND-CPs are in line with previous reports on the diffusion of adeno-associated virions in the cytoplasm of a living HeLa cell, where intracellular trajectories have also been classified as anomalous diffusion and normal diffusion. 54 It has been speculated that anomalous diffusion occurred due to nonspecific interactions between the virions and obstacles inside the cytoplasm such as organelles, which could also be the origin of the anomalous diffusion of ND-CPs. Therefore, ND-CPs consisting of a fluorescent nanodiamond core and capsid protein corona could represent valuable tools to elucidate the infection pathway the virions over the cytoplasm.…”

mentioning

confidence: 99%

Fluorescent Nanodiamonds Encapsulated by Cowpea Chlorotic Mottle Virus (CCMV) Proteins for Intracellular 3D-Trajectory Analysis

Wu¹,

Cao²,

Alam³

et al. 2021

Preprint

0

View full text Add to dashboard Cite

Long-term tracking of nanoparticles to resolve intracellular structures and motions is essential to elucidate fundamental parameters as well as transport processes within living cells. Fluorescent nanodiamond (ND) emitters provide cell compatibility and very high photostability. However, high stability, biocompatibility, and cellular uptake of these fluorescent NDs under physiological conditions are required for intracellular applications. Herein, highly stable NDs encapsulated with Cowpea chlorotic mottle virus capsid proteins (ND-CP) are prepared. A thin capsid protein layer is obtained around the NDs, which imparts reactive groups and high colloidal stability, while retaining the opto-magnetic properties of the coated NDs as well as the secondary structure of CPs adsorbed on the surface of NDs. In addition, the ND-CP shows excellent biocompatibility both in vitro and in vivo. Long-term 3D trajectories of the ND-CP with fine spatiotemporal resolutions are recorded; their intracellular motions are analyzed by different models, and the diffusion coefficient are calculated. The ND-CP with its brilliant optical properties and stability under physiological conditions provides us with a new tool to advance the understanding of cell biology, e.g., endocytosis, exocytosis, and active transport processes in living cells as well as intracellular dynamic parameters. <br>

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Diffusion Theory for the Infection Pathway of Virus in a Living Cell

Cited by 4 publications

References 18 publications

Gaussian fluctuation of the diffusion exponent of virus capsid in a living cell nucleus

Gaussian fluctuation of the diffusion exponent of virus capsid in a living cell nucleus

Fluorescent Nanodiamonds Encapsulated by Cowpea Chlorotic Mottle Virus (CCMV) Proteins for Intracellular 3D-Trajectory Analysis

Fluorescent Nanodiamonds Encapsulated by Cowpea Chlorotic Mottle Virus (CCMV) Proteins for Intracellular 3D-Trajectory Analysis

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