Diffusion tractography of the rat knee at microscopic resolution

Wang, Nian; Mirando, Anthony J.; Cofer, Gary P.; Qi, Yi; Hilton, Matthew J.; Johnson, G. Allan

doi:10.1002/mrm.27652

Cited by 29 publications

(52 citation statements)

References 63 publications

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“…19,20 Axial diffusivity (AD) and radial diffusivity (RD) were also derived using MatLab (MathWorks, Natick, MA) based on Diffusion Toolkit software. 21…”

Section: Imaging Analysismentioning

confidence: 99%

Probing demyelination and remyelination of the cuprizone mouse model using multimodality MRI

Wang

Zhuang

Wei

et al. 2019

Magnetic Resonance Imaging

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Background Various studies by MRI exhibit that the corpus callosum (CC) is the most vulnerable to cuprizone administration, detecting the demyelination and remyelination process using different MRI parameters are, however, lacking. Purpose To investigate the sensitivity of multiparametric MRI both in vivo and ex vivo for demyelination and remyelination. Study Type Prospective. Animal Model A cuprizone mice model with an age‐matched control group (n = 5), 4‐week cuprizone exposure group followed by 9‐week on a normal diet (n = 6), and a 13‐week cuprizone exposure group (n = 6). Field Strength/Sequence 3D gradient recalled echo, T2‐weighted, and diffusion tensor imaging (DTI) at 7.0T and 9.4T. Assessment Quantification of DTI metrics, quantitative susceptibility mapping (QSM), and T2‐weighted imaging intensity in major white matter bundles. Statistical Tests Nonparametric permutation tests were used with a cluster‐forming threshold as 3.09 (equivalent to P = 0.001), and the significant level as P = 0.05 with family‐wise correction. Results In vivo susceptibility values increased from –11.7 to –0.7 ppb (P < 0.001) in CC and from –13.7 to –5.1 ppb (P < 0.001) in the anterior commissure (AC) after the 13‐week cuprizone exposure. Ex vivo susceptibility values increased from –25.4 to 7.4 ppb (P < 0.001) in CC and from –41.6 to –15.8 ppb (P < 0.001) in AC. Susceptibility values showed high variations to demyelination for in vivo studies (94.0% in CC, 62.8% in AC). Susceptibility values exhibited higher variations than radial diffusivity for ex vivo studies (129.1% vs. 28.3% in CC, 62.0% vs. 25.0% in AC). In addition to the differential susceptibility variations in different white matter tracts, intraregional demyelination variation was also present not only in CC but also in the AC area by voxel‐based analysis. Data Conclusion QSM is sensitive to the demyelination process of cuprizone exposure, which can be a complementary technique to conventional T2‐weighted images and DTI metrics. Level of Evidence: 2 Technical Efficacy Stage: 2 J. Magn. Reson. Imaging 2019;50:1852–1865.

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“…19,20 Axial diffusivity (AD) and radial diffusivity (RD) were also derived using MatLab (MathWorks, Natick, MA) based on Diffusion Toolkit software. 21…”

Section: Imaging Analysismentioning

confidence: 99%

Probing demyelination and remyelination of the cuprizone mouse model using multimodality MRI

Wang

Zhuang

Wei

et al. 2019

Magnetic Resonance Imaging

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“…Two knee joints were harvested, shortly after sacrifice from 2 mature, healthy rats. The specimens were stained by immersion in a phosphate buffered saline solution of 0.5% Prohance (Bracco Diagnostics Inc., Princeton, NJ) to shorten T1 (to approximately 110 ms) and reduce scan time …”

Section: Methodsmentioning

confidence: 99%

“…The specimens were scanned at 9.4T Oxford 8.9‐cm vertical bore magnet (Agilent VnmrJ 4.0 imaging console) with maximum gradient strength of 2000 mT/m on each axis. Each specimen was fitted in an acrylic holder and then placed in a homemade solenoid radiofrequency coil (~3.0 cm × 1.3 cm × 1.3 cm) for MRI scans . A modified 3D Stejskal‐Tanner diffusion‐weighted spin‐echo pulse sequence was used with under‐sampling the phase dimensions by 4 times .…”

Section: Methodsmentioning

confidence: 99%

“…Each specimen was fitted in an acrylic holder and then placed in a homemade solenoid radiofrequency coil (~3.0 cm × 1.3 cm × 1.3 cm) for MRI scans . A modified 3D Stejskal‐Tanner diffusion‐weighted spin‐echo pulse sequence was used with under‐sampling the phase dimensions by 4 times . The varied density k‐space sampling pattern was illustrated in Supporting Information Figure .…”

Section: Methodsmentioning

confidence: 99%

“…DTI tractography has been used to detect the integrity of individual connective tissues, such as Achilles tendon, growth plate, and anterior cruciate ligament (ACL) graft in clinical scanners . Tractography of the articular cartilage in rat knee joint has also been reported with DTI at microscopic resolution …”

Section: Introductionmentioning

confidence: 99%

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Characterization complex collagen fiber architecture in knee joint using high‐resolution diffusion imaging

Wang

Mirando

Cofer

et al. 2020

Magnetic Resonance in Med

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School of Medicine (to N.W and C.E.S.).Purpose: To evaluate the complex fiber orientations and 3D collagen fiber network of knee joint connective tissues, including ligaments, muscle, articular cartilage, and meniscus using high spatial and angular resolution diffusion imaging. Methods: Two rat knee joints were scanned using a modified 3D diffusion-weighted spin echo pulse sequence with the isotropic spatial resolution of 45 μm at 9.4T. The b values varied from 250 to 1250 s/mm 2 with 31 diffusion encoding directions for 1 rat knee. The b value was fixed to 1000 s/mm 2 with 147 diffusion encoding directions for the second knee. Both the diffusion tensor imaging (DTI) model and generalized Q-sampling imaging (GQI) method were used to investigate the fiber orientation distributions and tractography with the validation of polarized light microscopy. Results: To better resolve the crossing fibers, the b value should be great than or equal to 1000 s/mm 2 . The tractography results were comparable between the DTI model and GQI method in ligament and muscle. However, the tractography exhibited apparent difference between DTI and GQI in connective tissues with more complex collagen fibers network, such as cartilage and meniscus. In articular cartilage, there were numerous crossing fibers found in superficial zone and transitional zone. Tractography generated with GQI also resulted in more intact tracts in articular cartilage than DTI. Conclusion: High-resolution diffusion imaging with GQI method can trace the complex collagen fiber orientations and architectures of the knee joint at microscopic resolution.

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Diffusion Tensor Imaging for Quantitative Assessment of Anterior Cruciate Ligament Injury Grades and Graft

Liu

Chen

et al. 2020

Magnetic Resonance Imaging

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Background As the need for quantitative assessment of anterior cruciate ligament (ACL) injuries and ACL graft increases, diffusion tensor imaging (DTI) becomes a more valuable measuring tool. However, DTI changes in differing injury grades of ACL and longitudinal graft remain unclear. Purpose To investigate the diagnostic performance of DTI in quantitatively assessing ACL injury severity and the development of ACL grafts within 6 months of surgery. Study Type A cohort study. Subjects Thirty‐five patients diagnosed with grades I–IV ACL injuries and 20 volunteers as controls were recruited. Field Strength/Sequence T1‐weighted, T2‐weighted, proton density (PD)‐weighted, and DTI at 3.0T MRI. Assessment ACL injury grades in arthroscopic images and DTI quantitative data were evaluated from July 2016 to July 2018. Statistical Tests Chi‐square test, analysis of variance, Spearman correlation analysis, and receiver operator characteristic (ROC) curves. Results Both fractional anisotropy (FA) (r = −0.898, P < 0.05) and apparent diffusion coefficient (ADC) (r = 0.851, P < 0.05) were significantly correlated with the severity of ACL injuries. The area under the curve (AUC) values for differentiation between low‐ and high‐grade ACL injuries with FA and ADC were 0.973 and 0.963, respectively. Although there were no significant differences in FA (P > 0.05) and ADC (P > 0.05) between grades I and II ACL injuries or in ADC (P > 0.05) between grades III and IV, there were significant differences in FA and ADC between two grades (P < 0.05). There were significant differences in FA (P < 0.05) and ADC (P < 0.05) between normal ACL and 3‐month graft postoperation, as well as in ADC values between 3‐month and 6‐month graft postoperation (P < 0.05). Data Conclusion DTI could be used to quantitatively evaluate the ACL injury grades and the development of ACL grafts. The diagnostic efficiency of FA values was higher than that of ADC values. Level of Evidence 1 Technical Efficacy Stage 3

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Diffusion tractography of the rat knee at microscopic resolution

Cited by 29 publications

References 63 publications

Probing demyelination and remyelination of the cuprizone mouse model using multimodality MRI

Probing demyelination and remyelination of the cuprizone mouse model using multimodality MRI

Characterization complex collagen fiber architecture in knee joint using high‐resolution diffusion imaging

Diffusion Tensor Imaging for Quantitative Assessment of Anterior Cruciate Ligament Injury Grades and Graft

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