Difructose Anhydride III Does Not Contribute to Body Energy Accumulation in Rats

Tamura, Akiko; Nino, Hadzuki; Minobe, Tomoko; Raneva, Violeta; Shigematsu, Norihiro; Hara, Hiroshi; Kishida, Taro; Ebihara, Kiyoshi

doi:10.1271/bbb.50666

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…The 3% or 6% DFAIII-containing diets included DFAIII at a ratio of 30 or 60 g/kg diet, respectively, replacing sucrose. Energy values of each diet were calculated using Atwater factors (4 kcal/g protein, 4 kcal/g carbohydrate, 9 kcal/g fat) and the energy value of DFAIII (0.263 kcal/g), 9) as follows: control diet, 3.63 kcal/g; 3% DFAIII-containing diet, 3.52 kcal/g; and 6% DFAIII-containing diet, 3.40 kcal/g. Body weight and food intake per weight were recorded daily for each rat in the morning before replenishing the diet.…”

Section: Animalsmentioning

confidence: 99%

Difructose anhydride III decreases body fat as a low-energy substitute or by decreasing energy intake in non-ovariectomized and ovariectomized female rats

Fujitani

Kishida

Shimizu

et al. 2017

Bioscience, Biotechnology, and Biochemistry

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We evaluated the effects of difructose anhydride III (DFAIII) on body weights of ovariectomized rats, which are a good model for obesity by estrogen deficiency-induced overeating. Female rats (10 weeks old) were subjected to ovariectomy or sham operation and then fed with or without a diet containing 3% or 6% DFAIII for 33 days or pair-fed control diet during the same period. Rats fed DFAIII showed significantly decreased food intake, energy intake, body weight gain, body energy accumulation, and fat tissue weight than control group, regardless of ovariectomy. DFAIII may decrease body fat dependent of reduced food/energy intake. Compared with the respective pair feeding groups, rats fed DFAIII showed significantly decreased body energy and fat tissue weight, regardless of ovariectomy, suggesting its potential as a low-energy substitute for high-energy sweeteners. The low energy of DFAIII may contribute to decreased body fat, which may not be dependent on obesity.

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Section: Animalsmentioning

confidence: 99%

Difructose anhydride III decreases body fat as a low-energy substitute or by decreasing energy intake in non-ovariectomized and ovariectomized female rats

Fujitani

Kishida

Shimizu

et al. 2017

Bioscience, Biotechnology, and Biochemistry

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“…We showed that it protected the integrity of the intestinal barrier in cattle and can be used as an alternative to protect against chronic low-dose mycotoxin contamination from cattle diets [ 19 , 20 ]. A previous report with experimental animals (rats) indicated that cecal SCFA concentrations in the DFA III-supplemented group were higher than those in the non-supplemented group under the same feeding conditions [ 21 , 22 ]. Although a large volume of SCFAs is produced through microbial fermentation of plant cellulose in the rumen, the dynamics of SCFAs in intestinal fermentation following the addition of DFA III as a supplement to cattle feeds are unknown.…”

Section: Introductionmentioning

confidence: 99%

Measurement of serum short-chain fatty acid concentrations in cattle after oral administration of difructose anhydride III

et al. 2023

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Background and Aim: We previously reported the mitigation effects of difructose anhydride III (DFA III) on mycotoxins, such as zearalenon and sterigmatocystin, based on the urinary concentrations of these molecules in calves. This study was aimed at evaluating the effects of dietary supplementation of DFA III and the fermented status of DFA III in the intestine by comparing serum levels of short-chain fatty acid (SCFAs) in DFA III-supplemented cattle with those in non-supplemented control cattle. Materials and Methods: Serum SCFA concentrations were measured in 30 Japanese Black heifers, aged 9–10 months, from two herds, using gas chromatography on days 0 (before DFA III supplementation), 9, and 14 after DFA III supplementation. Results: A notably different trend was observed for isobutyric acid and enanthic acid, which may reflect the different fermentation status of supplementary DFA III in the intestine. Our results indicate the possibility that this trend observed in the intestinal tract following DFA III administration is associated with changes in the environment of intestinal bacterial flora, which may partially reflect the effects of DFA III supplementation on cattle. Conclusion: Difructose anhydride III supplementation for at least 2 weeks affects the trend of blood SCFA concentrations in cattle. Our results provide evidence supporting the effects of DFA III on the intestinal environment and intestinal barrier function. Keywords: cattle, difructose anhydride III, short-chain fatty acid.

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“…DFAIII is high fermentable and enhances short-chain fatty acid (SCFA) synthesis during microbial fermentation in the cecum [17]. Intestinal acidification after DFAIII supplementation [18,19] increases DFAIIIassimilating bacteria and decreases fecal secondary BA concentration [20]. However, no information is available on BA metabolism, including enterohepatic circulation.…”

Section: Introductionmentioning

confidence: 99%

Ingestion of difructose anhydride III partially suppresses the deconjugation and 7α-dehydroxylation of bile acids in rats fed with a cholic acid-supplemented diet

Lee

Hori

Kohmoto

et al. 2019

Bioscience, Biotechnology, and Biochemistry

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Difructose anhydride III (DFAIII) is a prebiotic involved in the reduction of secondary bile acids (BAs). We investigated whether DFAIII modulates BA metabolism, including enterohepatic circulation, in the rats fed with a diet supplemented with cholic acid (CA), one of the 12α-hydroxylated BAs. After acclimation, the rats were fed with a control diet or a diet supplemented with DFAIII. After 2 weeks, each group was further divided into two groups and was fed diet with or without CA supplementation at 0.5 g/kg diet. BA levels were analyzed in aortic and portal plasma, liver, intestinal content, and feces. As a result, DFAIII ingestion reduced the fecal deoxycholic acid level via the partial suppression of deconjugation and 7α-dehydroxylation of BAs following CA supplementation. These results suggest that DFAIII suppresses production of deoxycholic acid in conditions of high concentrations of 12α-hydroxylated BAs in enterohepatic circulation, such as obesity or excess energy intake. Abbreviation: BA: bile acid; BSH: bile salt hydrolase; CA: cholic acid; DCA: deoxycholic acid; DFAIII: difructose anhydride III; MCA: muricholic acid; MS: mass spectrometry; NCDs: non-communicable diseases; LC: liquid chromatography; SCFA: short-chain fatty acid; TCA: taurocholic acid; TCDCA: taurochenodeoxycholic acid; TDCA: taurodeoxycholic acid; TUDCA: tauroursodeoxychlic acid; TαMCA: tauro-α-muricholic acid; TβMCA: tauro-β-muricholic acid; TωMCA: tauro-ω-muricholic acid

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Difructose Anhydride III Does Not Contribute to Body Energy Accumulation in Rats

Cited by 7 publications

References 33 publications

Difructose anhydride III decreases body fat as a low-energy substitute or by decreasing energy intake in non-ovariectomized and ovariectomized female rats

Difructose anhydride III decreases body fat as a low-energy substitute or by decreasing energy intake in non-ovariectomized and ovariectomized female rats

Measurement of serum short-chain fatty acid concentrations in cattle after oral administration of difructose anhydride III

Ingestion of difructose anhydride III partially suppresses the deconjugation and 7α-dehydroxylation of bile acids in rats fed with a cholic acid-supplemented diet

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