Digestion of Tannin by Bacteria Enterobacter cloacae from the Gut of Indian Mole Cricket (Gryllotalpa krishnani)

RK, Govindarajan; Revathi, S.; Rameshkumar, Neelamegam; Kayalvizhi, Nagarajan

doi:10.4172/2155-9821.1000302

Cited by 4 publications

(5 citation statements)

References 28 publications

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“…It has been observed that MSM media with 0.6% tannic acid at 37 °C promote the most optimal growth. Tannin-digesting bacteria Enterobacter cloacae (Rasiravuthanahalli et al, 2017), Paraburkholderia tropica PKI7, and Kosakonia arachidis PKI8 (Biswas & Mohapatra, 2023) showed maximum growth in MSM media with 0.6% tannic acid.…”

Section: Discussionmentioning

confidence: 99%

“…Tannase, a ubiquitous and important industrial enzyme in the microbial world, ability to degrade tannins to gallic acid and glucose (Anburaj, 2015). Different Bacterial strains degrading tannic acid were isolated from goat feces (Tahmourespour et al, 2016), ruminal bacteria (Cipriano-salazar et al, 2018), soil (Mohammed, 2016), and gut of Indian Mole Cricket (Rasiravuthanahalli et al, 2017). In this study, Enterobacter hormaechei strain Z8B-60 with the ability of tannic acid degradation was isolated from slaughterhouse waste enriched soil.…”

Section: Discussionmentioning

confidence: 99%

“…Degradation has no geographical or species limitations, just like resistance. Tannase producing bacterial isolates (Brahmbhatt & Modi, 2015), Klebsiella pneumoniae (Tahmourespour et al, 2016), Enterobacter cloacae (Rasiravuthanahalli et al, 2017) and Bacillus subtilis I-1 (Gloria Susan & Madhan, 2021) utilize tannins as energy source.…”

Section: Discussionmentioning

confidence: 99%

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Enterobacter hormaechei Z8b-60: A Tannin Degrading Bacteria Isolated from Slaughterhouse Waste

Narayanaswamy,

Hanumegowda,

Sadhasivam

et al. 2023

EJBIO

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Tannases or Tannin acyl hydrolases (EC 3.1.1.20) are a family of esterases that catalyze the hydrolysis of ester and depside bonds present in hydrolyzable tannins to release gallic acid. Industrial applications of tannase in food, brewing, chemical, pharmaceutical, and bioremediation, have made it one of the most important enzymes in research. The aim of the present study focused on the degradation of tannic acid by novel bacterial tannase isolated from slaughterhouse waste-enriched soil. Soil containing ruminant microflora is cultured on MSM containing tannic acid. Among four bacterial isolates, a potent strain was cultivated for identification which are then analysed for biochemical characterization and physiological characterization. Bacteria use tannic acid as the sole carbon source for growth and showed maximum growth at 0.6%. The morphology of the isolate shows that it contains gram-negative rods that are nonmotile and capsulated. Based on 16S rRNA and phylogenetic analysis, the isolate was confirmed as Enterobacter hormaechei Z8b-60. Tannase production from bacteria was confirmed by a novel, sensitive plate assay by observing the zone of clearance around colonies and also by dye binding method using methyl gallate as substrate. During the study, TLC and HPLC were performed to analyze the accumulated gallic acid and other metabolites. Intracellular tannase production amplified from 14.98 U/mL to 102.7 U/mL as tannic acid concentration increases from 0.4% to 1.0%, respectively.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Enterobacter hormaechei Z8b-60: A Tannin Degrading Bacteria Isolated from Slaughterhouse Waste

Narayanaswamy,

Hanumegowda,

Sadhasivam

et al. 2023

EJBIO

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“…Enterobacter is common in functional flora of insect gut, such as Bactrocera dorsalis ( 55 , 56 ), S. litura ( 34 ), Spodoptera littoralis ( 57 ), and Gryllotalpa krishnani ( 58 ). Enterobacteriaceae is one of the most widespread families across 30 lepidopteran insects, while the Enterobacter is one of the most common genera ( 59 ).…”

Section: Discussionmentioning

confidence: 99%

Gut bacteria mediated adaptation of diamondback moth, Plutella xylostella, to secondary metabolites of host plants

Xia,

Wang,

Gurr

et al. 2023

mSystems

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The diamondback moth (DBM), Plutella xylostella, has successfully adapted to the potent chemical defenses of Brassicaceae plants that deter most other herbivores. Gut bacteria are increasingly recognized as key to the biology of many species but their role in DBM adaptation to plant defense compounds is not well known. In this study, the secondary metabolites of radish seedlings, rich in flavonoids, were identified by liquid chromatography-mass spectrometry. These secondary metabolites reduced the larval growth of DBM lacking gut bacteria. The effect was rapidly eclipsed by the re-introduction of gut microbiota, which was dominated by Enterobacter (Proteobacteria). Similarly, while treatment with the flavonoid kaempferol adversely affected growth and extended the development time, these were alleviated by the re-introduction of Enterobacter sp. EbPXG5 (EbPXG5) to the DBM gut. EbPXG5 not only degrades kaempferol both in vitro and DBM gut, but is also shown to colonize the gut epithelium, forming a protective biofilm. Genomic sequencing of EbPXG5 showed that metabolic genes were the most abundant, especially those involved in xenobiotic degradation, and the metabolism of terpenoids and polyketides, which could participate in the degradation of plant secondary metabolites such as kaempferol. Overall, our results showed that EbPXG5 is a bacterium common in the gut of DBM larvae and has the in vitro and in vivo capacity to detoxify a major secondary metabolite that is produced in brassica plants as a defense against herbivores. This insect-bacterial association may be an important contributor to the status of DBM as a major pest of brassica crops worldwide. IMPORTANCE In this study, we identify an important role of gut bacteria in mediating the adaptation of diamondback moth (DBM) to plant secondary metabolites. We demonstrate that kaempferol’s presence in radish seedlings greatly reduces the fitness of DBM with depleted gut biota. Reinstatement of gut biota, particularly Enterobacter sp. EbPXG5, improved insect performance by degrading kaempferol. This bacterium was common in the larval gut of DBM, lining the epithelium as a protective film. Our work highlights the role of symbiotic bacteria in insect herbivore adaptation to plant defenses and provides a practical and mechanistic framework for developing a more comprehensive understanding of insect-gut microbe-host plant co-evolution.

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“…Isolated from the gut of Indian male cricket (Govindarajan et al, 2017) Exhibits tannase activity, shielding the host insect from the antinutritional action of the plant tannins. One of the three highly abundant bacteria in the gut of diamondback moth, Plutella xylotella (Xia et al, 2017) Metagenomic analysis indicates that they are involved in the production of enzymes involved in amino acid synthesis, digestion of plant products, and detoxification.…”

Section: Enterobacter Cloacaementioning

confidence: 99%

Culturable Endosymbiotic Bacteria From the Indian Lac Insect, Kerria Lacca (Kerr)

Verma,

Ramani,

Chandra

et al. 2024

Ina.J.For.Res

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The Indian lac insect, Kerria lacca (Kerr) (Coccoidea: Tachardiidae) is a commercially important phytosuccivorous and sessile scale insect. Lac insects are cultured on suitable host plants in India and some Southeast Asian countries to produce lac. The lac insect harbours a number of endosymbionts. Isolation of culturable microbial endosymbionts and their identification through 16S rRNA has revealed sex and host-related differences of microbial species. Bacillus boroniphilus, Enterobacter cloacae and Staphylococcus sp. were found only in the lac insects reared on the plant host Cajanus cajan, whereas Bacillus firmus, Lysinibacillus xylanilyticus, Bacillus horneckiae and Bacillus velezensis were recorded only from Flemingia macrophylla. B. firmus and L. xylanilyticus were female-specific and B. horneckiae and B. velezensis were male-specific with Flemingia macrophylla as host; E. cloacae was female-specific and Bacillus boroniphilus and Staphylococcus sp. were male specific with C. cajan. Biochemical characteristics of the isolates, their genetic relationship with their taxonomic kin and their probable role, based on the information available about these endosymbionts in other hosts, have been studied.

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Digestion of Tannin by Bacteria Enterobacter cloacae from the Gut of Indian Mole Cricket (Gryllotalpa krishnani)

Cited by 4 publications

References 28 publications

Enterobacter hormaechei Z8b-60: A Tannin Degrading Bacteria Isolated from Slaughterhouse Waste

Enterobacter hormaechei Z8b-60: A Tannin Degrading Bacteria Isolated from Slaughterhouse Waste

Gut bacteria mediated adaptation of diamondback moth, Plutella xylostella, to secondary metabolites of host plants

Culturable Endosymbiotic Bacteria From the Indian Lac Insect, Kerria Lacca (Kerr)

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